Co-relation of salivary calcium and vitamin D with dental caries - an ex-vivo study

Authors

DOI:

https://doi.org/10.51248/.v42i5.1706

Keywords:

Dental caries, saliva, calcium, vitamin D, oral health, demineralization

Abstract

Introduction and Aim: Calcium and vitamin D3 play a pivotal role in many biological functions like hard tissue mineralization and tooth formation. Both are dietary elements with a known concentration in the saliva, therefore directly affecting the rate and progression of dental caries. The aim of the study was to compare the salivary vitamin D and calcium levels in caries active and caries free adult patients.

 

Materials and Methods: Tarson’s saliva collection tubes were used to collect 5 ml of saliva samples from 110 patients. The supernatant obtained from the centrifuged saliva was stored at -4°C. Analysis of calcium levels was done using the Arsenazo method. Commercially available vitamin D Elisa kit (Epitope diagnostics) was used for the analysis of vitamin D levels.

 

Results: The mean salivary vitamin D levels were higher in the caries free group (66.57mg/dl) when compared to the caries active group (56.43mg/dl). The mean salivary calcium level was elevated in the caries free group (27.09mg/dl) when compared to the caries active group (6.84 mg/dl).

 

Conclusion: The filtrates in saliva play a major role in the occurrence of dental caries through a demineralization and remineralization cycle. Vitamin D in saliva causes a decrease in bacterial aggregation and biofilm formation through the protective role of peptides, causing decreased demineralization of the tooth surface and increased level of free calcium ions in the saliva.

Author Biographies

Nireeksha

Department of Conservative Dentistry and Endodontics, A.B. Shetty Memorial Institute of Dental Sciences, Nitte (deemed to be) University, Mangalore 575018, Karnataka, India

Nidhi Hegde

Department of Conservative Dentistry and Endodontics, A.B. Shetty Memorial Institute of Dental Sciences, Nitte (deemed to be) University, Mangalore 575018, Karnataka, India

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Published

2022-11-14

How to Cite

1.
Nireeksha, Hegde N. Co-relation of salivary calcium and vitamin D with dental caries - an ex-vivo study. Biomedicine [Internet]. 2022 Nov. 14 [cited 2022 Nov. 27];42(5):966-9. Available from: https://biomedicineonline.org/home/article/view/1706

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