Molecular analysis of acid-stable alpha-amylase (asAA) gene in Aspergillus niger using PCR-RFLP


  • Majid Zarrin
  • Zahra Shialy



Acid-stable alpha-amylase (alphasAA) gene, Aspergillus niger, PCR-RFLP


Introduction and Aim: Amylase is an important enzyme with vast applications in various industries such as food and therapeutic industries. Aspergillus niger is commercially engaged in the making of alpha-amylase. Acid-stable alpha -amylase is mostly produced with microorganisms such as Bacillus and Aspergillus. The aim of this research was the molecular investigation of the acid-stable alpha-amylase (alpha-sAA) gene in A. niger.


Materials and Methods: Sixty-three A. niger isolates were evaluated in this study.  PCR method was performed for amplification of a 347 bp DNA band of the alpha-sAA gene.  The Hpa II Restriction endonuclease was used for the digestion of PCR fragments.


Results: A 347 bp DNA fragment was recovered from 49 out of 63 (78%) isolates. After cutting the PCR products with the HpalphaII enzyme, 81.6% of isolates showed the expected band and 18.4% presented different restriction endonuclease patterns.


Conclusion: The results demonstrated the PCR-RFLP technique performed in this research was a valuable tool for analysis of the alpha-sAA gene in A. niger isolates.

Author Biographies

Majid Zarrin

Infectious and Tropical Diseases Research Center, Health Research Institute, Department of Medical Mycology,Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Zahra Shialy

Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran


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How to Cite

Zarrin M, Shialy Z. Molecular analysis of acid-stable alpha-amylase (asAA) gene in Aspergillus niger using PCR-RFLP. Biomedicine [Internet]. 2022 Nov. 14 [cited 2022 Nov. 27];42(5):970-2. Available from:



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