Effect of centrifugation force and time on the analysis of lactate dehydrogenase and potassium in the serum samples

Authors

  • Veena S. Charantimath
  • Vanishree S. Bubanale

DOI:

https://doi.org/10.51248/.v42i5.1915

Keywords:

Turnaround time, centrifugation, lactate dehydrogenase, potassium

Abstract

Introduction and Aim: Any imperfection that occurs during any stage of the testing process is described as laboratory error. Increasing requirements of biochemical tests, numerous patient samples and automation has forced laboratory work to be carried out at a faster speed. Few studies are shown to investigate the influence of settings of centrifugation of less than 10 minutes on the laboratory result in serum. Thus, our study was aimed to see the effect of centrifugation force and time on the analysis of lactate dehydrogenase(LDH) and potassium from serum samples.

 

Methodology: Samples were collected from 61 healthy volunteers. 5ml was taken in two separate BD vacutainer serum tubes. Tube 1 was centrifuged for 2000g for 10 minutes, tube 2 for 5 minutes 3000g, and analysed for LDH and potassium.

 

Results: A significant difference was observed between 5 min (U/L) (3000g) and 10 min (U/L) (2000g) with LDH and 5 min (mmol/l) 3000g and  10 min (mmol/l) 2000g with potassium.

 

Conclusion: LDH and potassium levels were found to be raised by increasing the centrifugal force to 3000g. Hence, the standard centrifugation protocol of 10 min at 2000 or 2500 rpm is to be followed to get the accurate results.

Author Biographies

Veena S. Charantimath

Department of Biochemistry, KAHERs J. N. Medical College, Belagavi, Karnataka, India

 

Vanishree S. Bubanale

Department of Biochemistry, KAHERs J. N. Medical College, Belagavi, Karnataka, India

 

References

Institute of Medicine (US) Committee on Quality of Health Care in America Kohn LT, Corrigan JM, Donaldson MS, editors. To Err is Human: Building a Safer Health System. Washington (DC): National Academies Press (US); 2000. [Google Scholar]

Kaushik, N., Green, S. Pre-analytical errors: their impact and how to minimize them. MLO Med Lab Obs. 2014; 46(5):22-24.

Mohammedsaleh, Z.M., Mohammedsaleh, F. A review article of the reduce errors in medical laboratories. Global Journal of Health Science. 2015; 7(1):46. DOI: https://doi.org/10.5539/gjhs.v7n1p46

Plebani, M. Diagnostic errors and laboratory medicine–causes and strategies. The Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (eJIFCC). 2015; 26(1):7.

Oostendorp, M., Van Solinge, W.W., Kemperman, H. Potassium but not lactate dehydrogenase elevation due to in vitro hemolysis is higher in capillary than in venous blood samples. Archives of Pathology and Laboratory Medicine. 2012; 136(10):1262-1265. DOI: https://doi.org/10.5858/arpa.2011-0319-OA

Koseoglu, M., Hur, A., Atay, A., Cuhadar, S. Effects of hemolysis interference on routine biochemistry parameters. Biochemia Medica. 2011; 21(1):79-85. DOI: https://doi.org/10.11613/BM.2011.015

Wan Norlina, W.A., Omar, J., Koon, T.S., Tuan Salwani, T.I. Hemolyzed Specimens: Major Challenge for Identifying and Rejecting Specimens in Clinical Laboratories. Oman Medical Journal. 2019; 34(2):94-98. DOI: https://doi.org/10.5001/omj.2019.19

Mensel, B., Wenzel, U., Roser, M., Ludemann, J., Nauck, M. Considerably Reduced Centrifugation Time without Increased Hemolysis: Evaluation of the New BD Vacutainer® SST™ II Advance. Clinical Chemistry. 2007; 53(4):794-795. DOI: https://doi.org/10.1373/clinchem.2006.079582

Padoan, A., Zaninotto, M., Piva, E., Sciacovelli, L., Aita, A., Tasinato, A., et al., Quality of plasma samples and BD Vacutainer Barricor tubes: effects of centrifugation. Clinica Chimica Acta. 2018; 483: 271-274. DOI: https://doi.org/10.1016/j.cca.2018.05.018

Miler, M., Gabaj, N.N., Culej, J., Unic, A., Vrtaric, A., Kopcinovic, L.M. Integrity of serum samples is changed by modified centrifugation conditions. Clinical Chemistry and Laboratory Medicine (CCLM). 2019; 57(12):1882-1887. DOI: https://doi.org/10.1515/cclm-2019-0244

Lesche, D., Geyer, R., Lienhard, D., Nakas, C.T., Diserens, G., Vermathen, P., et al., Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome. Metabolomics. 2016; 12(10):1-6. DOI: https://doi.org/10.1007/s11306-016-1109-3

Compton, C.C., Robb, J.A., Andrew, M.W., Berry, A.B., Birdsong, G.G., Bloom, K.J., et al., Preanalytical and precision pathology practices to ensure molecular integrity of cancer patient biospecimens for precision medicines. Archives of Pathology and Laboratory Medicine. 2019; 143(11):1346-1363. DOI: https://doi.org/10.5858/arpa.2019-0009-SA

Klin, Z. Standardization of methods for the determination of enzyme activities in biological fluids. Chem Klin Biochem 1972 Jun;10(6):281-291.

Weishaar H. D., Prasad, M.C., Parker R.S. Estimation of lactate dehydrogenase in serum/plasma. Med. Welt. 1975; 26:387-391.

Ashwood, B. Bruns & Saunders : Tietz Text Book of Clinical Chemistry 4th Edition -2006.

Ruzicka, J., Hansen, E.H., Zagatto, E.A. Flow injection analysis, Part VII. Use of ion selective electrodes for rapid analysis of soil extracts and blood serum. Determination of potassium, sodium and nitrate. Anal. Chim. Acta, 1977, 88, 1-16. DOI: https://doi.org/10.1016/S0003-2670(01)96043-X

Badiou, S., Vuillot, O., Bargnoux, A.S., Kuster, N., Lefebvre, S., Sebbane, M., et al., Improved quality of samples and laboratory turnaround time using 3.5 mL low vacuum BD Vacutainer Barricor tubes in the emergency department. Practical Laboratory Medicine. 2019;16:00128. DOI: https://doi.org/10.1016/j.plabm.2019.e00128

Monneret, D., Corlouer, C., Bigot, J., Atlan, G., Alkouri, R., Mestari, F., et al., Comparison of a 10-vs. 15-min centrifugation time for chemical and immunochemical assays and impact on turnaround time in a hospital laboratory. Clinical Chemistry and Laboratory Medicine (CCLM). 2016;54(4):e117-e121. DOI: https://doi.org/10.1515/cclm-2015-0664

Bausset, O., Juvet, O., Staller-Gobelli, C., Milano, E., Bausset, J.P. Impact of serumclot contact time on lactate dehydrogenase and inorganic phosphorus serum levels. Practical Laboratory Medicine. 2017;7:36-40. DOI: https://doi.org/10.1016/j.plabm.2017.01.002

Simundic, A.M., Nikolac, N., Ivankovic, V., Ferenec-Ruzic, D., Magdic, B., Kvaternik, M. Comparison of visual vs. automated detection of lipemic, icteric and hemolyzed specimens: can we rely on a human eye? Clinical Chemistry and Laboratory Medicine. 2009;47(11):1361-1365. DOI: https://doi.org/10.1515/CCLM.2009.306

Downloads

Published

2022-11-14

How to Cite

1.
S. Charantimath V, S. Bubanale V. Effect of centrifugation force and time on the analysis of lactate dehydrogenase and potassium in the serum samples. Biomedicine [Internet]. 2022 Nov. 14 [cited 2022 Nov. 27];42(5):1094-7. Available from: https://biomedicineonline.org/home/article/view/1915

Issue

Section

Short Communications

Plum Analytics