Detection of anti-testicular antibodies among infertile males using indirect immunofluorescent technique


  • Rana Hazim Hamoode
  • Sami A. Alkubaisy
  • Dalal A. Sattar
  • Sawsan S. Hamzah
  • Tahreer Hadi Saleh
  • Bahaa Abdullah Laftaah Al-Rubaii



Antitesticular antibody, infertile males, oligospermia, duration of marriage


Introduction and Aim: The antinuclear antibodies (ANA) are unwanted molecules which bind and destroy certain structures within the nucleus. Immunofluorescence is a powerful technique that utilizes fluorescent-labeled antibodies to detect specific target antigens. The aim of this study was to detect the anti-testicular antibody among infertile males in Baghdad city and determine the most common type of infertility.


Materials and Methods: The study involved 73 male (53 infertile and 20 non-infertile) volunteers, at Kamal Al-Samarrai Teaching Hospital in Baghdad, Iraq. Serum collected from the study subjects was tested for steroid-cell antibodies (STC-Ab), anti-nuclear antibody (ANA) and anti-testicular antibodies (ATCA) by Indirect Immunofluorescence assay (IIFA). Data obtained was subjected to statistical analysis using the SPSS program.


Results: In the current study 52.9% of infertile men tested positive for testicular antibodies compared to the control group. The highest rate of testicular cell antibodies was observed in the serum of infertile patients aged between 30-39 years and the lowest in patients aged 50-59 years. The marriage duration among those showing the highest percentage of antibodies against testicular cells was 1-10 years. Study for the association of male infertility type to those positive for ATCA showed almost all (85.7%) patients with necrozoospermia to be positive for ATCA. This was followed by males with azoospermia (50%) and oligospermia (46.9%). The patients were negative for Addison’s disease while a few (28.6%) were positive for lupus erythematosus.

Author Biographies

Rana Hazim Hamoode

Basic Science Department, Dentistry of College, University of Anbar, Anbar, Iraq

Sami A. Alkubaisy

Desert Studies Center, University of Anbar, Anbar, Iraq

Dalal A. Sattar

Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq

Sawsan S. Hamzah

College of Dentistry, Al-Farahidi University, Baghdad, Iraq

Tahreer Hadi Saleh

Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq

Bahaa Abdullah Laftaah Al-Rubaii

Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq



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How to Cite

Hazim Hamoode R, A. Alkubaisy S, A. Sattar D, S. Hamzah S, Hadi Saleh T, Laftaah Al-Rubaii BA. Detection of anti-testicular antibodies among infertile males using indirect immunofluorescent technique. Biomedicine [Internet]. 2022 Nov. 14 [cited 2022 Nov. 27];42(5):978-82. Available from:



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