Evaluation of anti-inflammatory activity of Garcinia indica, a wild edible fruit by inhibiting secretory phospholipase A2 group IIA enzyme from human pleural fluid
Keywords:Garcinia indica, Secretory phospholipase A2, wild edible fruit, anti-inflammatory, antioxidant
Introduction and Aim: Garcinia indica is a wild edible fruit plant distributed in tropical regions of India. Fruits of G. indica were traditionally used to treat chronic inflammatory diseases like rheumatoid arthritis, gastrointestinal disorders, etc., But the basis of anti-inflammatory function of the fruit is not understood. Therefore, this study aims to evaluate the anti-inflammatory function of G. indica. Initially phytochemical screening of G. indica was carried out, tested antioxidant potency of G. indica fruit and evaluated its anti-inflammatory function by inhibition of secretory phospholipase A2 IIA (sPLA2IIA) enzyme.
Methodology: The Soxhlet extraction method was used for the preparation of extracts of G. indica fruit. DPPH and phosphomolybdenum assays were carried out to estimate the antioxidant activities of G. indica fruit. The inhibition of sPLA2IIA, modulating indirect hemolytic and edema inducing activity and protein denaturation assays were done to evaluate the anti-inflammatory function.
Results: Aqueous and solvent extracts of G. indica fruit showed a wide variety of phytochemicals. The ethanol extract of G. indica fruit showed greater antioxidant activity (79.12 % ±1.2) and reduction power (68.14% ± 4.4). The extract showed sPLA2IIA inhibition to a greater extent (50%), neutralized sPLA2IIA induced indirect hemolysis (IC50 45.518 µg/mL) and mouse paw edema (119.35% ± 2.74) with the IC50 value of 45.12 ±1.36µg.
Conclusion: The extracts of G. indica contain a wide variety of phytochemicals. The ethanol extract of G. indica fruit exhibited a greater antioxidant activity and anti-inflammatory activity. Thus, the results defended the traditional usage of the G. indica fruit in the indigenous system of medicine.
Burke, J. E., Dennis, E. A. Phospholipase A2 structure/function, mechanism, and signaling. Journal of lipid research. 2009 Apr 1;50(Supplement): S237-S242. DOI: https://doi.org/10.1194/jlr.R800033-JLR200
Dennis, E. A., Cao, J., Hsu, Y.H., Magrioti, V., Kokotos, G. Phospholipase A2 enzymes: physical structure, biological function, disease implication, chemical inhibition, and therapeutic intervention. Chemical reviews. 2011 Sep 12; 111(10): 6130-6185. DOI: https://doi.org/10.1021/cr200085w
Yarla, N. S., Satyakumar, K., Srinivasu, D., Kaladhar, D.S.V.G.K., Aliev, G., Dharmapuri, G., et al., Phospholipase A2: a potential therapeutic target in inflammation and cancer (In silico, In vitro, In vivo and clinical approach). J Cancer Sci Ther. 2015; 7:8.
Butterfield, D. A., Hensley, K., Cole, P., Subramaniam, R., Aksenov, M., Aksenova, M., et al., Oxidatively induced structural alteration of glutamine synthetase assessed by analysis of spin label incorporation kinetics: relevance to Alzheimer's disease. Journal of neurochemistry. 1997 Jun; 68(6): 2451-2457. DOI: https://doi.org/10.1046/j.1471-4159.1997.68062451.x
Lewen, A., Matz, P., Chan, P. H. Free radical pathways in CNS injury. Journal of Neurotrauma. 2000 Oct; 17(10): 871-890. DOI: https://doi.org/10.1089/neu.2000.17.871
Götz, M. E., Künig, G., Riederer, P., Youdim, M. B. Oxidative stress: free radical production in neural degeneration. Pharmacology & Therapeutics. 1994 Jan 1; 63(1): 37-122. DOI: https://doi.org/10.1016/0163-7258(94)90055-8
Goldman, R., Moshonov, S., Chen, X., Berchansky, A., Fürstenberger, G., Zor, U. Crosstalk between elevation of [Ca 2+] i, reactive oxygen species generation and phospholipase A 2 stimulation in a human keratinocyte cell line. In Recent Advances in Prostaglandin, Thromboxane, and Leukotriene Research. 1997; (pp. 41-45). Springer, Boston, MA. DOI: https://doi.org/10.1007/978-1-4899-1810-9_7
FitzGerald, G. A. Coxibs and cardiovascular disease. N. Engl. J. Med. 2004; 351: 1709-1711. DOI: https://doi.org/10.1056/NEJMp048288
Giresha, A. S., Urs, D., Pundalik, S., Meti, R. S., Pramod, S. N., Supreetha, B. H., et al., Sinapic acid Inhibits Group IIA Secretory Phospholipase A2 and Its Inflammatory Response in Mice. Antioxidants. 2022 Jul; 11(7): 1251. DOI: https://doi.org/10.3390/antiox11071251
Barnes, J., Anderson, L. A., Phillipson, J. D. Herbal Medicines, 3rd ed.; Pharmaceutical Press: London, UK, 2007; pp. 1-23.
Nordstrom, C. R. Exploring pluralism—the many faces of Ayurveda. Social science & medicine. 1988 Jan 1; 27(5): 479-489. DOI: https://doi.org/10.1016/0277-9536(88)90371-1
Baliga, M. S., Bhat, H. P., Pai, R. J., Boloor, R., Palatty, P. L. The chemistry and medicinal uses of the underutilized Indian fruit tree Garcinia indica Choisy (kokum): a review. Food Research International. 2011 Aug 1; 44(7): 1790-1799. DOI: https://doi.org/10.1016/j.foodres.2011.01.064
Blois, M. S. Antioxidant determinations by the use of a stable free radical. Nature. 1958 Apr; 181(4617): 1199-1200. DOI: https://doi.org/10.1038/1811199a0
Vishwanath, B. S., Fawzy, A.A., Franson, R.C. Edema-inducing activity of phospholipase A2 purified from human synovial fluid and inhibition by aristolochic acid. Inflammation. 1988 Dec;12(6): 549-561. DOI: https://doi.org/10.1007/BF00914317
Gutiérrez, J., Avila, C., Rojas, E., Cerdas, L. An alternative in vitro method for testing the potency of the polyvalent antivenom produced in Costa Rica. Toxicon. 1988 Jan 1; 26(4): 411-413. DOI: https://doi.org/10.1016/0041-0101(88)90010-4
Boman, H. G., Kaletta, U. Chromatography of rattlesnake venom A separation of three phosphodiesterases. Biochimica Biophysica Acta. 1957 Jan 1; 24: 619-631. DOI: https://doi.org/10.1016/0006-3002(57)90256-1
Vishwanath, B.S., Gowda, T.V. Interaction of aristolochic acid with Vipera russelli phospholipase A2: its effect on enzymatic and pathological activities. Toxicon. 1987 Jan 1;25(9):929-937. DOI: https://doi.org/10.1016/0041-0101(87)90155-3
Panda, V., Ashar, H., Srinath, S. Antioxidant and hepatoprotective effect of Garcinia indica fruit rind in ethanol-induced hepatic damage in rodents. Interdisciplinary toxicology. 2012 Dec; 5(4):20. DOI: https://doi.org/10.2478/v10102-012-0034-1
Ahmad, A., Wang, Z., Ali, R., Maitah, M.Y., Kong, D., Banerjee, S. Apoptosis- inducing effect of garcinol is mediated by NF-kappaB signaling in breast cancer cells, J Cell Biochem. 2010; 109(6):1134-1141. DOI: https://doi.org/10.1002/jcb.22492
Hope, W. C., Patel, B. J., Fiedler-Nagy, C., Wittreich, B. H. Retinoids inhibit phospholipase A2 in human synovial fluid and arachidonic acid release from rat peritoneal macrophages. Inflammation. 1990 Oct;14(5):543-559. DOI: https://doi.org/10.1007/BF00914275
Giresha, A.S., Urs, D., Manjunatha, J.G., Sophiya, P., Supreetha, B.H., Jayarama, S., et al., Group IIA secreted phospholipase A2 inhibition by elemolic acid as a function of anti-inflammatory activity. Scientific Reports. 2022 May 10;12(1):1-6. DOI: https://doi.org/10.1038/s41598-022-10950-1
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