Evaluation of anti-inflammatory activity of Garcinia indica, a wild edible fruit by inhibiting secretory phospholipase A2 group IIA enzyme from human pleural fluid

Authors

  • Narayanappa M.
  • Deepdarshan Urs
  • Sathisha A. D.
  • Krishna Ram H.
  • Rajkumar S. Meti
  • Dharmappa K. K.

DOI:

https://doi.org/10.51248/.v42i5.2173

Keywords:

Garcinia indica, Secretory phospholipase A2, wild edible fruit, anti-inflammatory, antioxidant

Abstract

Introduction and Aim: Garcinia indica is a wild edible fruit plant distributed in tropical regions of India. Fruits of G. indica were traditionally used to treat chronic inflammatory diseases like rheumatoid arthritis, gastrointestinal disorders, etc., But the basis of anti-inflammatory function of the fruit is not understood. Therefore, this study aims to evaluate the anti-inflammatory function of G. indica. Initially phytochemical screening of G. indica was carried out, tested antioxidant potency of G. indica fruit and evaluated its anti-inflammatory function by inhibition of secretory phospholipase A2 IIA (sPLA2IIA) enzyme.

Methodology: The Soxhlet extraction method was used for the preparation of extracts of G. indica fruit. DPPH and phosphomolybdenum assays were carried out to estimate the antioxidant activities of G. indica fruit. The inhibition of sPLA2IIA, modulating indirect hemolytic and edema inducing activity and protein denaturation assays were done to evaluate the anti-inflammatory function.

Results: Aqueous and solvent extracts of G. indica fruit showed a wide variety of phytochemicals. The ethanol extract of G. indica fruit showed greater antioxidant activity (79.12 % ±1.2) and reduction power (68.14% ± 4.4). The extract showed sPLA2IIA inhibition to a greater extent (50%), neutralized sPLA2IIA induced indirect hemolysis (IC50 45.518 µg/mL) and mouse paw edema (119.35% ± 2.74) with the IC50 value of 45.12 ±1.36µg.

Conclusion: The extracts of G. indica contain a wide variety of phytochemicals. The ethanol extract of G. indica fruit exhibited a greater antioxidant activity and anti-inflammatory activity. Thus, the results defended the traditional usage of the G. indica fruit in the indigenous system of medicine.

 

Author Biographies

Narayanappa M.

Inflammation Research Laboratory, Department of Studies and Research in Biochemistry, Mangalore University Jnana Kaveri Post Graduate Center, Chikka Aluvara, 571232, Kodagu, Karnataka, India

Deepdarshan Urs

Inflammation Research Laboratory, Department of Studies and Research in Biochemistry, Mangalore University Jnana Kaveri Post Graduate Center, Chikka Aluvara, 571232, Kodagu, Karnataka, India

Sathisha A. D.

Department of Biochemistry, Sampurna International Institute of Agricultural Sciences and Horticulture Technology, K. B. Doddi-Belekere, Maddur, Mandya, 562160, Karnataka, India

Krishna Ram H.

Nisarga Research and Development trust(T), Yadagondanhalli, Hosakote Taluk, Bengaluru,560011 Karnataka, India

 

Rajkumar S. Meti

Inflammation Research Laboratory, Department of Studies and Research in Biochemistry, Mangalore University Jnana Kaveri Post Graduate Center, Chikka Aluvara, 571232, Kodagu, Karnataka, India

Dharmappa K. K.

Inflammation Research Laboratory, Department of Studies and Research in Biochemistry, Mangalore University Jnana Kaveri Post Graduate Center, Chikka Aluvara, 571232, Kodagu, Karnataka, India

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Published

2022-11-14

How to Cite

1.
M. N, Urs D, A. D. S, Ram H. K, S. Meti R, K. K. D. Evaluation of anti-inflammatory activity of Garcinia indica, a wild edible fruit by inhibiting secretory phospholipase A2 group IIA enzyme from human pleural fluid. Biomedicine [Internet]. 2022 Nov. 14 [cited 2022 Nov. 27];42(5):1058-64. Available from: https://biomedicineonline.org/home/article/view/2173

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