Polysaccharide hydrolyzing enzyme activity of bacteria, native to Apis florea gut

Authors

  • D. N. Ganeshprasad Department of Studies in Biochemistry, Mangalore University, Jnana Kaveri Post Graduate Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India https://orcid.org/0000-0002-1526-1110
  • Yalpi Karthik Department of Studies in Microbiology, Mangalore University, Jnana Kaveri Post Graduate Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India https://orcid.org/0000-0002-0097-613X
  • H. R. Sachin Department of Studies in Biochemistry, Mangalore University, Jnana Kaveri Post Graduate Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India https://orcid.org/0000-0001-6537-7624
  • A. H. Sneharani Department of Studies in Biochemistry, Mangalore University, Jnana Kaveri Post Graduate Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India https://orcid.org/0000-0001-7209-7540

DOI:

https://doi.org/10.51248/.v41i4.1013

Keywords:

Honey bees, extracellular enzymes, gut bacteria, carbohydrates, fermentation

Abstract

Introduction and Aim: Apis florea commonly known as “dwarf honey bee” harbors enormous gut bacteria that can digest complex carbohydrates and other food components. In this regard, the present investigation was focused on analyzing the polysaccharide degrading ability of bacteria isolated from the gut of honeybee, for their possible application in nutraceutical and pharmaceutical industries.

 

Materials and Methods: Nine bacterial isolates were screened for carbohydrate degrading enzymes viz., amylase, pectinase, cellulase, tannase and laccase, using respective substrate by plate assay method. Further activities of amylase and pectinase were measured quantitatively by dinitrosalicylic acid (DNS) method.

 

Results: All the nine selected isolates exhibited amylase and pectinase activities. However, only two isolates exhibited lignolytic and cellulolytic activity. None of the isolates showed tannin degradation. Maximum amylase activity (4.95 U/mg) was observed in Bacillus halotolerans af-M9 followed by Klebsiella oxytoca af-G4 (4.62 U/mg). With respect to pectinase activity Klebsiella pneumoniae af-E17 displayed higher activity (0.24 U/mg) followed by Klebsiella oxytoca af-G4 (0.20 U/mg).

 

Conclusion: Habitat-specific innovations are being explored for novel compounds for therapeutic applications. This study throws a light on selection of carbohydrate degrading bacteria from a new source i.e., GUT of honeybee.

 

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Published

2021-12-31

How to Cite

1.
Ganeshprasad DN, Karthik Y, Sachin HR, Sneharani AH. Polysaccharide hydrolyzing enzyme activity of bacteria, native to Apis florea gut. Biomedicine [Internet]. 2021Dec.31 [cited 2022Jan.20];41(4):768-75. Available from: https://biomedicineonline.org/index.php/home/article/view/1013

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Original Research Articles