Polysaccharide hydrolyzing enzyme activity of bacteria, native to Apis florea gut


  • D. N. Ganeshprasad Department of Studies in Biochemistry, Mangalore University, Jnana Kaveri Post Graduate Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India https://orcid.org/0000-0002-1526-1110
  • Yalpi Karthik Department of Studies in Microbiology, Mangalore University, Jnana Kaveri Post Graduate Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India https://orcid.org/0000-0002-0097-613X
  • H. R. Sachin Department of Studies in Biochemistry, Mangalore University, Jnana Kaveri Post Graduate Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India https://orcid.org/0000-0001-6537-7624
  • A. H. Sneharani Department of Studies in Biochemistry, Mangalore University, Jnana Kaveri Post Graduate Centre, Chikka Aluvara, 571232, Kodagu, Karnataka, India https://orcid.org/0000-0001-7209-7540




Honey bees, extracellular enzymes, gut bacteria, carbohydrates, fermentation


Introduction and Aim: Apis florea commonly known as “dwarf honey bee” harbors enormous gut bacteria that can digest complex carbohydrates and other food components. In this regard, the present investigation was focused on analyzing the polysaccharide degrading ability of bacteria isolated from the gut of honeybee, for their possible application in nutraceutical and pharmaceutical industries.


Materials and Methods: Nine bacterial isolates were screened for carbohydrate degrading enzymes viz., amylase, pectinase, cellulase, tannase and laccase, using respective substrate by plate assay method. Further activities of amylase and pectinase were measured quantitatively by dinitrosalicylic acid (DNS) method.


Results: All the nine selected isolates exhibited amylase and pectinase activities. However, only two isolates exhibited lignolytic and cellulolytic activity. None of the isolates showed tannin degradation. Maximum amylase activity (4.95 U/mg) was observed in Bacillus halotolerans af-M9 followed by Klebsiella oxytoca af-G4 (4.62 U/mg). With respect to pectinase activity Klebsiella pneumoniae af-E17 displayed higher activity (0.24 U/mg) followed by Klebsiella oxytoca af-G4 (0.20 U/mg).


Conclusion: Habitat-specific innovations are being explored for novel compounds for therapeutic applications. This study throws a light on selection of carbohydrate degrading bacteria from a new source i.e., GUT of honeybee.



Hall, A. B., Tolonen, A. C., Xavier, R. J. Human genetic variation and the gut microbiome in disease. Nat Rev Genet. 2017 Nov; 18(11): 690-699. doi: 10.1038/nrg.2017.63. Epub 2017 Aug 21. PMID: 28824167.

Engel, P., Moran, N. The gut microbiota of insects – diversity in structure and function. FEMS Microbiology Reviews. 2013; 37(5): 699-735.

Flint, H. J., Bayer, E. A., Rincon, M. T., Lamed, R., White, B.A. Polysaccharide utilization by gut bacteria: potential for new insights from genomic analysis. Nature reviews. Microbiology 2008; 6(2): 121-131.

Zheng, H., Perreau, J., Powell, J, E., Han, B., Zhang, Z., Kwong, W. K., et al., Division of labor in honeybee gut microbiota for plant polysaccharide digestion. Proc Natl Acad Sci U S A. 2019 Dec 17; 116(51): 25909-25916. doi: 10.1073/pnas.1916224116. Epub 2019 Nov 27. PMID: 31776248; PMCID: PMC6926048.

Kwong, W., Moran, N. Gut microbial communities of social bees. Nat Rev Microbiol. 2016 Jun 1;14(6): 374-384.

Munn, C. Microbes in the Marine Environment. Marine Microbiology Ecology and Applications. London?;New York, N. Y.?;Independence, KY?: Garland Science/BIOS Scientific Publishers?;Distributed in the USA by Fulfilment Center, Taylor & Francis. 2004: 1-24 p.

Fenice, M., Gallo, A. M., Juarez-Jimenez, B., Gonzalez-Lopez, J. Screening for extracellular enzyme activities by bacteria isolated from samples collected in the Tyrrhenian Sea. Ann Microbiol. 2007 [cited 2021 Aug 19]; 57(1): 93-99.

Krishnan, M., Bharathiraja, C., Pandiarajan, J., Prasanna, V. A., Rajendhran, J., Gunasekaran, P. Insect gut microbiome - An unexploited reserve for biotechnological application. Asian Pac J Trop Biomed. 2014; 4(Suppl 1): S16-S21.

Gerhardt, P., Murray, R.G.E., Wood, W. A., Kreig, N. R. Methods for general and Molecular Bacteriology. ASM, Washington, DC; 1994.

Bandounas, L., Wierckx, N. J., de Winde, J. H., Ruijssenaars, H. J. Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential. BMC Biotechnol. 2011; [cited 2021 Aug 19]; 11(1): 1-11.

Andro, T., Chambost, J. P., Kotoujansky, A., Cattaneo, J., Bertheau, Y., Barras, F., et al., Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase. J Bacteriol. 1984 [cited 2021 Aug 19]; 160(3): 1199.

Unban, K., Kodchasee, P., Shetty, K., Khanongnuch, C. Tannin-tolerant and extracellular tannase producing bacillus isolated from traditional fermented tea leaves and their probiotic functional properties. Foods. 2020; 9(4).

Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov; 193(1): 265-275. PMID: 14907713.

Oluoch, K., Okanya, P., Hatti-Kaul, R., Mattiasson, B., Mulaa, F. Protease-, pectinase- and amylase- producing bacteria from a Kenyan soda lake. The Open Biotechnology Journal. 2018; 12(1): 33-45.

Balachandra, C., Subash Chandran, M. D., Ramachandra, T.V. Honeybee Diversity, Role in Pollination and Beekeeping Scenario in South Indian Western Ghats. Sahyadri E-News: Western Ghats Biodiversity Information System, ENVIS 2014; 46: 1-23.

Engel, P., Martinson, V. G., Moran, N. A. Functional diversity within the simple gut microbiota of the honey bee. Proc Natl Acad Sci U S A. 2012; 109(27): 11002-11007.

Kwong, W. K., Moran, N. A. Gut Microbial Communities of Social Bees. Nat Rev Microbiol. 2016 Jun 1 [cited 2021 Aug 19]; 14(6): 374. Available from: /pmc/articles/PMC5648345/

Lee, F. J., Rusch, D. B., Stewart, F. J., Mattila, H. R., Newton I. L. G. Saccharide breakdown and fermentation by the honey bee gut microbiome. Environ Microbiol. 2015; 17(3): 796-815.

Choi, S. M., Park, M. H., Jung, T. S., Moon, K. H., Kim, K. M., Kang, J. S. Characterization of Bacillus mojavensis KJS-3 for industrial applications. Arch Pharmacal Res 2011 342. 2011 Mar 6 [cited 2021 Sep 2]; 34(2): 289-298. Available from: https://link.springer.com/article/10.1007/s12272-011-0215-z

Dorra, G., Ines, K., Imen, B. S., Laurent, C., Sana, A., Olfa, T., et al., Purification and characterization of a novel high molecular weight alkaline protease produced by an endophytic Bacillus halotolerans strain CT2. Int J Biol Macromol. 2018 May 1; 111: 342-351.

Vaidya, S., Rathore, P. Isolation, screening and characterization of amylase producing bacteria from soil of potato dump sites from different regions of Madhya pradesh. Int Conf Recent Trends Agri Vet Life Sci. 2015; 1(7): 1-7.

Uchima, C. A., Tokuda, G., Watanabe, H., Kitamoto, K., Arioka, M. Heterologous expression and characterization of a glucose-stimulated ?-glucosidase from the termite Neotermes koshunensis in Aspergillus oryzae. Appl Microbiol Biotechnol. 2011 Mar; 89(6): 1761-1771. doi: 10.1007/s00253-010-2963-y. Epub 2010 Nov 6. PMID: 21057947.

Kannan, M., Suryaaathmanathan, V., Saravanakumar, M., Jaleel, A., Romanelli, D., Tettamanti, G., et al. Proteomic analysis of the silkworm midgut during larval-pupal transition. Invertebr Surviv J. 2016; 13(June): 191-204.

Kannan, M., Mubarakali, D., Thiyonila, B., Krishnan, M., Padmanaban, B., Shantkriti, S. Insect gut as a bioresource for potential enzymes - an unexploited area for industrial biotechnology. Biocatal Agric Biotechnol. 2019 Mar 1; 18: 101010.

Martinson, V. G., Magoc, T., Koch, H., Salzberg, S. L., Moran, N. A. Genomic features of a bumble bee symbiont reflect its host environment. Applied and Environmental Microbiology. 2014 Jul 1; 80(13): 3793-803.




How to Cite

Ganeshprasad DN, Karthik Y, Sachin HR, Sneharani AH. Polysaccharide hydrolyzing enzyme activity of bacteria, native to Apis florea gut. Biomedicine [Internet]. 2021Dec.31 [cited 2022Jan.20];41(4):768-75. Available from: https://biomedicineonline.org/index.php/home/article/view/1013



Original Research Articles