Diagnostic accuracy of maternal serum endothelial-derived nitric oxide synthase (eNOS) and its correlation with birth outcomes, in preeclampsia - a case-control study


  • Sangappa Virupaxappa Kashinakunti
  • Basavaraj Devaranavadagi
  • Manjula Rangappa
  • Ashalata Mallapur




Preeclampsia, nitric oxide synthase, diagnosis


Introduction and Aim: Pre­eclampsia (PE) results from impaired placentation, leading to placental hypoxia and dysfunction and abnormalities in the endothelial nitric oxide synthase (eNOS)-nitric oxide pathway. The present study was undertaken to estimate and compare maternal serum eNOS concentrations in women with PE and normal healthy pregnant women, to know the correlation of eNOS level with severity of PE, to find the best cut-off value for the diagnosis of PE and to study its association of eNOS levels with the birth outcome.

Material and Methods: This was a case-control study, conducted at a tertiary care hospital. The study was approved by the Institutional ethics committee. Informed consent was obtained from all the participants. Primigravida (singleton) pregnant women aged 18-35 years, 20 or more weeks of gestation, diagnosed as PE and classified as mild/severe PE based on America college of obstetricians and gynecologists guidelines were selected for the study. eNOS was estimated by the ELISA method. All the participants were visited again to note the outcomes. 

Results:The maternal serum eNOS in PE was significantly lower than the control group (p=0.019). The best cut-off value was 187.25pg/ml to diagnose PE, area under the ROC curve (AUC) 0.61, sensitivity 95.1%, specificity 37.5% and diagnostic accuracy was 66,67%. Birth outcomes did not have a significant correlation with eNOS.

Conclusion: eNOS was significantly lowerer in PE, it can be used as a diagnostic marker with the best cut of value of 187.25 pg/ml.


Author Biographies

Sangappa Virupaxappa Kashinakunti

Department of Biochemistry, Shri B M Patil Medical College, BLDE University, Vijayapur-586101, Karnataka, India


Department of Biochemistry, S. Nijalingappa Medical College, Navanagar, Bagalkot-587102 Karnataka, India

Basavaraj Devaranavadagi

Department of Biochemistry, Shri B M Patil Medical College, BLDE University, Vijayapur-586101, Karnataka, India

Manjula Rangappa

 Department of Community Medicine,  S. Nijalingappa Medical College, Navanagar, Bagalkot-587102 Karnataka, India

Ashalata Mallapur

Professor and Head Department of Obstetrics and Gynaecology, S. Nijalingappa Medical College, Navanagar, Bagalkot-587102 Karnataka, India


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How to Cite

Kashinakunti SV, Devaranavadagi B, Rangappa M, Mallapur A. Diagnostic accuracy of maternal serum endothelial-derived nitric oxide synthase (eNOS) and its correlation with birth outcomes, in preeclampsia - a case-control study. Biomedicine [Internet]. 2021Sep.7 [cited 2021Sep.22];41(2):344-8. Available from: https://biomedicineonline.org/index.php/home/article/view/1037

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