Evaluation of plasma PDGF and VEGF levels after systemic administration of activated autologous platelet-rich plasma

Authors

  • Karina Karina
  • Krista Ekaputri
  • Johannes Albert Biben
  • Imam Rosadi
  • Iis Rosliana
  • Siti Sobariah
  • Sulaeha AD
  • Hubert Andrew
  • Irsyah Afini
  • Tias Widyastuti
  • Jusryanti Jusryanti
  • Sristin Indah Prestiani
  • Indah Mustika Donna
  • MeylaShinta Mutiara
  • Habibi Habibi

DOI:

https://doi.org/10.51248/.v41i2.1047

Keywords:

Activated autologous platelet-rich plasma (aaPRP), platelet derived growth factor (PDGF), vascular endothelial growth factor (VEGF)

Abstract

Introduction and Aim: Activated autologous platelet-rich plasma (aaPRP) is becoming a popular therapy to accelerate healing in the field of plastic surgery. Platelets, which are abundant in aaPRP, can release many growth factors including platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). This study aims to examine the plasma levels of PDGF and VEGF in healthy subjects after intravenous administration of aaPRP.

 

Materials and Methods: Nine healthy patients with no prior history of metabolic disease were divided into two groups (control and experiment group). The treatment group which consists of six patients received intravenous aaPRP treatment. The preparation of aaPRP starts with the collection of 24 mL of whole blood in sodium citrate tubes followed a two-step centrifugation procedure and subsequent chemical activation. aaPRP was then administered intravenously to patients. Meanwhile, the control group received no intervention. Venous blood samples were taken before and one week after the treatment and the plasma PDGF and VEGF levels were determined by enzyme-linked immunosorbent assay (ELISA).

 

Results: The treatment group showed statistically significant increase in VEGF after 7 days compared to before aaPRP administration. However, the change in PDGF levels of the treatment group was unnotable.

 

Conclusion: The present findings indicate that intravenous administrations of activated aaPRP may increase plasma VEGF level up to 1 week after aaPRP administration.

Author Biographies

Karina Karina

Klinik Hayandra, Hayandra Lab, Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Faculty of Medicine, Pusat Kajian Stem Cell, Universitas Pembangunan Nasional Veteran Jakarta 12450, Indonesia

Krista Ekaputri

Klinik Hayandra, Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Johannes Albert Biben

Klinik Hayandra,  Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Imam Rosadi

Hayandra Lab, Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University Samarinda 752424, Indonesia

Iis Rosliana

Hayandra Lab, Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Siti Sobariah

Hayandra Lab, Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Sulaeha AD

Klinik Hayandra, Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Hubert Andrew

Hayandra Lab, Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Universitas Indonesia, Jl. Salemba Raya no. 6, Jakarta Pusat 10430, Indonesia

Irsyah Afini

Hayandra Lab, Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Tias Widyastuti

Hayandra Lab, Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Jusryanti Jusryanti

Klinik Hayandra, Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Sristin Indah Prestiani

Klinik Hayandra, Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Indah Mustika Donna

Klinik Hayandra,  Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

MeylaShinta Mutiara

Klinik Hayandra, Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

Habibi Habibi

Klinik Hayandra,  Yayasan HayandraPeduli, Jl. Kramat VI No. 11, Jakarta Pusat 10430, Indonesia

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Published

2021-09-07

How to Cite

1.
Karina K, Ekaputri K, Biben JA, Rosadi I, Rosliana I, Sobariah S, AD S, Andrew H, Afini I, Widyastuti T, Jusryanti J, Prestiani SI, Donna IM, Mutiara M, Habibi H. Evaluation of plasma PDGF and VEGF levels after systemic administration of activated autologous platelet-rich plasma. Biomedicine [Internet]. 2021Sep.7 [cited 2021Sep.22];41(2):409-12. Available from: https://biomedicineonline.org/index.php/home/article/view/1047