The modulating effect of ferulic acid on high fat diet induced hyperlipidemia and obesity:A dose response study in male Sprague Dawleyrats


  • Mumtaz Khan Mohamed
  • V. Ramamurthy



HMG CoA reductase, adiponectin, blood lipids


Introduction and Aim: Ferulic acid (FA) is a phenolic compound predominantly found in cereals have been used in traditional Chinese medicine. Here, we studied the effect of FA on high-fat diet (HFD) induced hyperlipidemia and obesity in rats.


Materials and Methods:Hyperlipidemia was induced in male Sprague Dawley rats by feeding HFD for 14 weeks. The hypolipidemic effect was evaluated by co-administering 50,100, 200 and 250 mg/kg body weight of FA. At the end of the experimental period, rats were sacrificed and serum/plasma levels of total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), aspartate transaminase (AST), alanine transaminase (ALT), 3-hydroxy-3methyl-glutaryl-coenzyme A reductase (HMG CoA reductase) and adiponectin were determined. Moreover, Histopathological examination of liver and visceral adipose tissue (AT) was also carried out.


Results:HFD treatment significantly increased weight gain, body mass index, total fat pad mass, blood lipids, LDL cholesterol and serum transaminases.HFD +FA fed rats showed a significant decrease in blood lipids and an increase in antioxidant enzymes when compared to the HFD control rats. The activity of HMG CoA reductase and serum adiponectin levels were elevated in rats administered with FA. Among the 4 doses studied, 200 mg of FA/kg body weight exhibited optimum hypolipidemic activity. Histological observations in the liver and visceral AT added additional evidence for the lipid-lowering effect of FA.


Conclusion:These findings indicate that FA can act as a hypolipidemic agent, probably by modulating the activity of HMG CoA reductase and serum adiponectin levels.

Author Biographies

Mumtaz Khan Mohamed

Postgraduate and Research Department of Biochemistry, Marudupandiyar College(Affiliated to Bharathidasan University),Thanjavur - 613 403,Tamil Nadu, India

V. Ramamurthy

Postgraduate and Research Department of Biochemistry, Marudupandiyar College(Affiliated to Bharathidasan University),Thanjavur - 613 403,Tamil Nadu, India


Gloria, L. World Health Organization-Obesity. The editors of EncyclopediaBritannica, May 16, 2019.

Hruby, A., Hu, F.B. The epidemiology of obesity: A big picture. Pharmacoeconomics. 2015; 33: 673-689.

Peirce, V., Pellegrinelli, V., Vidal-Puig, A. Adipose Structure (White, Brown, Beige). InAhima R.S. (eds) Metabolic Syndrome. Springer, Cham; 2016.

Achari, A.E., Jain, S.K. Adiponectin, a therapeutic target for obesity, diabetes, and endothelial dysfunction. Int J Mol Sci. 2017;18(6) :1321.

Ulla, A., Alam, M. A., Sikder, B., Sumi, F. A., Rahman, M. M, Habib, Z. F., et al., Supplementation of Syzygiumcumini seed powder prevented obesity, glucose intolerance, hyperlipidemia and oxidative stress in high carbohydrate high fat diet induced obese rats. BMC complementary and alternative medicine. 2017 Dec;17(1):1-3.

Dragan, M., Stan, C. D., Iacob, A. T., Dragostin, O., Profire, L. Ferulic acid: potential therapeutic applications. Rev Med Chir Soc Med Nat Iasi. 2018; 122(2): 388-395.

Bezerra, G., Pereira, M., Ostrosky, E., Barbosa, E., Moura, M., Ferrari, M.,et al.,Compatability study between ferulic acid and excipients used in cosmetic formulations by TG/DTG, DSC and FTIR. J Therm Anal Calorim. 2017; 127: 1683-1691.

Nascimento, A.F., Sugizaki, M.M., Leopoldo, A.S., Lima-Leopoldo, A.P., Luvizotto, R.A., Nogueria, C.R. A hyper caloric pellet-diet cycle induces obesity and co-morbidities in Wistar rats. Araq. Bras. Endocrinol. Metab. 2008; 52: 968-974.

Phillip, B., Shapiro, D.J. Improved methods for the assay and activation of 3-hydroxy-3-methyl glutaryl coenzyme A reductase. J. Lipid. Res. 1970; 20:588.

Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976; 582(1): 67-78.

Zak,B., Dickenman, R.C., White, E.G., Burnett, U., Cherney, P.J. Rapid estimation of free and total cholesterol. Am. J. Clin. Pathol. 1954; 24: 1307-1315.

Van Handel, E., Zilversmit, D.B. Micro method for the direct determination of serum triglyceride. J. Lab. Clin. Med. 1957;50: 152-157.

Kuchmak, M., Hazlehurst, J.S., Olanshy, A.S., Taylor, L. Reference sera with graded levels of high-density lipoprotein cholesterol. Clin. Chim. Acta., 1984; 144: 237-243.

Bairaktari, E.T., Seferiadis, K.I., Elisaf, M.S. Evaluation of methods for the measurement of low-density lipoprotein cholesterol. J. Cardiovasc. Pharmacol. Ther., 2005; 10: 45-54.

Reitman, S., Frankel, S.A. Colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvate transaminases. Am. J. Clin. Pathol., 1957; 28: 56.

Flohe, L.,Gunzler, W.A. Assay of glutathione peroxides. Methods. Enzymol. 1984; 105:114-121.

Kakkar, P., Das, B., Viswanathan, PN. A modified spectrophotometric assay of superoxide dismutase. Indian. J. Biochem. Biophys., 1984; 21: 130-132.

Aebi, H. Catalase in vitro. Methods. Enzymol. 1984; 105:121.

Bancroft, J.D., Gamble, M. Theory and Practice of Histological Techniques 5th edition. Ch6: Tissue processing and microtome including frozen, Ch8: The Hematoxylins and Eosin Churchill Livingston Elsevier, Philadelphia, USA, pp: 85-97, 125.

Pankaj, G., Sanjay, J. Isolation, characterization, and hypolipidemic activity of ferulic acid in high fat-diet induced hyperlipidemia in laboratory rats. EXCLI. J., 2016; 599-613.

Nawaboot, J., Piyabhan, P., Munkong, N., Parklak, W., Pannangpetch, P. Ferulic acid improves lipid and glucose homeostasis in high-fat- diet-induced obese mice.Clin Exp Pharma Col. 2016; 43: 242-250.

Salazar-López, N. J., Astiazarán-García, H., González-Aguilar, G.A., Loarca-Piña, G., Ezquerra-Brauer, J.M., Domínguez Avila, J.A., et al., Ferulic Acid on Glucose Dysregulation, Dyslipidemia, and Inflammation in Diet-Induced Obese Rats: An Integrated Study. Nutrients. 2017; 9: 675.

Marseglia, L., Manti, S., D'Angelo, G.,Nicotera, A., Parisi, E., Di Rosa, G., et al., Oxidative stress in obesity: a critical component in human diseases. Int J Mol Sci. 2014; 16(1): 378-400.

Alam, M. A. Anti-hypertensive effect of cereal antioxidant ferulic acid and its mechanism of action. Front. Nutr. 2018; 6: 121.




How to Cite

Mohamed MK, Ramamurthy V. The modulating effect of ferulic acid on high fat diet induced hyperlipidemia and obesity:A dose response study in male Sprague Dawleyrats. Biomedicine [Internet]. 2021Sep.7 [cited 2021Sep.22];41(2):413-20. Available from: