Evaluation of anti-oxidative properties of <i>Tinosporacordifoliain Alloxan</i> induced diabetic Wistar rats


  • Kumud Ranjan Thakur
  • S.R. Padmadeo
  • Vikash Kumar




Glucose, Tinospora cordifolia, diabetes, Alloxan, antioxidant


Introduction and Aim: Type 2 Diabetes is a complex heterogenous disorder with marked hyperglycemia, reduced insulin sensitivity, increased oxidative stress, and altered carbohydrate, fat and protein metabolism. Increased oxidative stress is a key factor for the reduced pancreatic ? cell arose due to prolonged hyperglycemia. The present investigation admits ethanolic extract of Tinosporacordifolia (250mg/kg/body weight) as a potent antihyperglycemic agent as well as antioxidant.


Materials and methods: Wistar norvegicus rats of 180-200 gm were involved in the present study divided in to 5 groups. Each group has 5 rats. Diabetes was induced by Alloxan (100mg/kg.b.wt). Administration of T.cordifolia (250mg/kg/b.wt) done in the desired group for 10,20, and 30 days respectively. Fasting blood collected for plasma glucose and the organs were collected for the antioxidant assay.


Results: Each group showed significant recovery in the glucose and antioxidant parameters like Glutathione, Catalase, Ascorbate etc., Statistical analysis was done by Tukey multiple range test compared with entire column after ANOVA. Level of significance was denoted for diabetic Vs treated group having p values *** p<0.05, **p<0.01, *p<0.001, NS-Non-significant, >0.05.


Conclusion: Relying on the Herbal extract of T.cordifolia would be one of the best options for controlling glucose concentration, enhanced immunity, and getting rid of unnecessary free radicals which increases aging. The plant under investigation is a reservoir of countless secondary metabolites.

Author Biographies

Kumud Ranjan Thakur

Department of Biochemistry Patna University,Patna, Bihar, India

S.R. Padmadeo

Post Graduate Department of Botany, Patna University,Patna, Bihar, India

Vikash Kumar

Department of Biochemistry, RMRIMS, Agamkuan, Patna, Bihar, India


Elksnis, A., Martinell, M., Eriksson, O., Espes, D. Heterogeneity of Metabolic Defects in Type 2 Diabetes and Its Relation to Reactive Oxygen Species and Alterations in Beta-Cell Mass. Front Physiol.2019;10:107.

Khalil, H. Diabetes microvascular complications-A clinical update. Diabetes MetabSyndr. 2017; 11 Suppl 1:S133-S139.

Palumbo, C., Nicolaci, N., La Manna, A.A., Branek, N., Pissano, M.N. Association between central diabetes insipidus and type 2 diabetes mellitus. Medicina (B Aires). 2018;78(2):127-130.

World health Organization (WHO). Global report on Diabetes 2016

Rehman, K., Akash, M.S.H. Mechanism of generation of oxidative stress and pathophysiology of type 2 diabetes mellitus: how are they interlinked?.J Cell Biochem. 2017;118(11):3577-3585.

Ansar, S., Iqbal, M., Athar, M. Nordihydroguairetic acid is a potent inhibitor of ferric-nitrilotriacetate-mediated hepatic and renal toxicity, and renal tumour promotion, in mice. Carcinogenesis. 1999 Apr 1;20(4):599-606.

Sinha, A.K. Colorimetric assay of catalase. Anal Biochem. 1972; 47(2):389-394.

Boyne, A.F., Ellman, G.L. A methodology for analysis of tissue sulfhydryl components. Anal Biochem. 1972; 46(2):639-653.

Ansar, S., Iqbal, M., Athar, M. Nordihydroguairetic acid is a potent inhibitor of ferric-nitrilotriacetate-mediated hepatic and renal toxicity, and renal tumour promotion, in mice. Carcinogenesis. 1999 Apr 1;20(4):599-606.

Newman, D.J., Cragg, G.M., Snader, K.M. The influence of natural products upon drug discovery. Nat. Prod. Rep.2000; 17: 215-234.

Omaye, S.T., Turnbull, J.D., Sauberlich, H.E. Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids.MethodsEnzymol. 1979; 62:3-11.

Rotruck, J.T., Pope, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G., Hoekstra, W.G. Selenium: biochemical role as a component of glutathione peroxidase.Science. 1979 Feb 9; 179 (4073):588-590.

Habig, W.H., Pabst, M.J.,Jakoby, W.B. Glutathione S-transferases. The firstJournal of Biochemistry.1974;47: 469-474.

Marklund, S.,Marklund, G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase.Eur J Biochem. 1974 Sep 16; 47(3):469-474.

Sharma, B.R., Park, C.M., Kim, H.A., Kim, H.J., Rhyu, D.Y. Tinosporacordifolia preserves pancreatic beta cells and enhances glucose uptake in adipocytes to regulate glucose metabolism in diabetic rats. Phytother Res. 2019; 33(10):2765-2774.

Aluwong, T., Ayo, J.O., Kpukple, A., Oladipo, O.O. Amelioration of hyperglycaemia, oxidative stress and dyslipidaemia in alloxan-induced diabetic wistar rats treated with probiotic and vitamin C. Nutrients. 2016;8(5):151.

?uraševi?, S., Jasni?, N., Proki?, M., Grigorov, I., Martinovi?, V., ?or?evi?, J., et al., The protective role of virgin coconut oil on the alloxan-induced oxidative stress in the liver, kidneys and heart of diabetic rats. Food & function. 2019;10(4):2114-2124.

Sangeetha, M.K., Balaji Raghavendran, H.R., Gayathri, V., Vasanthi, H.R. Tinosporacordifolia attenuates oxidative stress and distorted carbohydrate metabolism in experimentally induced type 2 diabetes in rats. J Nat Med. 2011; 65(3-4):544-550.

Reddi, K.K., Tetali, S.D. Dry leaf extracts of Tinosporacordifolia (Willd.) Miers attenuate oxidative stress and inflammatory condition in human monocytic (THP-1) cells. Phytomedicine. 2019; 61:152831.

Ghosh, D.S., Jagatheesan, G., Haberzettl, P., Shah, J., Hill, BG., Bhatnagar, A., et al.,Glutathione S transferase P deficiency induces glucose intolerance via JNK dependent enhancement of hepatic gluconeogenesis. Am J PhysiolEndocrinol Metal. 2018.

Baskaran, R., Priya, L.B., Sathish Kumar, V., Padma, V.V. Tinosporacordifolia extract prevents cadmium-induced oxidative stress and hepatotoxicity in experimental rats. J Ayurveda Integr Med. 2018;9(4):252-257.

Wang, Y., Branicky, R., Noë, A., Hekimi, S. Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling. J Cell Biol. 2018;217(6):1915-1928.

Priya, L.B., Baskaran, R., Elangovan, P., Dhivya, V., Huang, C.Y., Padma, V.V. Tinosporacordifolia extract attenuates cadmium-induced biochemical and histological alterations in the heart of male Wistar rats. Biomed Pharmacother. 2017; 87:280-287.

Ates, I., Kaplan, M., Inan, B.,Alisik, M.,Erel, O., Yilmaz, N., et al., How does thiol/disulfide homeostasis change in prediabetic patients? Diabetes Res Clin Pract. 2015;110(2):166-171.

Requejo, R., Chouchani, E.T., Hurd, T.R., Menger, K.E., Hampton, M.B., Murphy, M.P. Measuring mitochondrial protein thiol redox state. Methods Enzymol. 2010; 474:123-147.

Spoelstra-de Man, A.M.E., Elbers, P.W.G., Oudemans-Van Straaten, H.M. Vitamin C: should we supplement? CurrOpinCrit Care. 2018; 24(4):248-255.

Madani, Z., Sener, A., Malaisse, W.J., Dalila, A.Y. Sardine protein diet increases plasma glucagon-like peptide-1 levels and prevents tissue oxidative stress in rats fed a high-fructose diet. Mol Med Rep. 2015; 12(5):7017-7026.

Som, S., Basu, S., Mukherjee, D., Deb, S., Choudhury, P.R., Mukherjee, S.,et al., Ascorbic acid metabolism in diabetes mellitus. Metabolism 1981; 30: 572-577.




How to Cite

Thakur KR, Padmadeo S, Kumar V. Evaluation of anti-oxidative properties of <i>Tinosporacordifoliain Alloxan</i> induced diabetic Wistar rats. Biomedicine [Internet]. 2021Sep.7 [cited 2021Sep.22];41(2):421-6. Available from: https://biomedicineonline.org/index.php/home/article/view/1050