Synthesis, characterization and biomedical applications of silver nanoparticles

Authors

  • Sabah Saad Abdulsahib

DOI:

https://doi.org/10.51248/.v41i2.1058

Keywords:

Nanobiotechnology, antimicrobial, toxicity activity, tumor therapy

Abstract

Introduction and Aim:Silver nanoparticles (AgNPs) have been extensively useful in biomedical applications.This study aims to synthesize silver nanoparticles by lasers ablation and to use them as an anti-bacterial and anti-cancer agent.

 

Materials and Methods:According to the current study, Ag-nanoparticles can be synthesized easily using pulsed laser ablation on a 99.81% pure silver target immersed in deionized water. The prepared nanoparticles werecharacteredbyUV-vis spectroscopy and X-ray diffraction (XRD), transmission electron microscopy (TEM), and atomic force microscopy (AFM) technique.Using a well-diffusion process, the anti-bacterial action of the synthesized Ag nanoparticles was tested versus two Gram-positive bacteria species (S. aureus and S. pyogenes) and two species of Gram-negative bacteria (E. coli and P. aeruginosa).The anticancer activity of the silver nanoparticles was evaluated by the MTT assay.

 

Results:The generated AgNps had a maximum absorbance peak of 400 nm. The XRD analysis verified that the synthesized silver nanoparticles had been nanocrystalline. The AgNPsdid not affect any of the blood parameters. Gram-negative bacteria are more affected by silver than Gram-positive bacteria. The Ag nanoparticles had been shown a maximum anti-bacterial action at a concentration of 80 µg/mland had a lower effect with 20 µg/ml concentration while their efficacy at 40 and 60 µg/ml concentrationsappeared to be variable against all bacterial species.The findings show that AgNPshavea cytotoxic influence on cancer cellsin 80 ?g/ml concentration.

 

Conclusion:In comparison to Gram-positive bacteria, silver nanoparticles show high antibacterial activity against Gram-negative bacteria. The prepared nanoparticles have a potent effect on cancer cells and restricted harmful effects on RBCs.

Author Biography

Sabah Saad Abdulsahib

Lecturer, Biomedical Engineering Department, University of Technology - Iraq, Baghdad, Iraq

References

Hamouda, R.A., Hussein, M.H., Abo-elmagd, R.A., Bawazir, S.S. Synthesis and biological characterization of silvernanoparticles derived from the cyanobacterium Oscillatoria limnetica. Scientific Reports. 2019; 9:13071.

Ndikau, M.,Noah, N.M., Andala, D.M., Masika, E. Green synthesis and characterization of silver nanoparticles using Citrullus lanatus fruit rind extract. International Journal of Analytical Chemistry. 2017;2017: 8108504.

Espinosa, J.C.M., Cerritos, R.C., Morales, M.A.R., Guerrero, K.P.S., Contreras, R.A.S., Macías, J.H. Characterization of silver nanoparticles obtained by a green route and their evaluation in the bacterium of Pseudomonas aeruginosa. Crystals. 2020; 10: 395.

Anandalakshmi, K.,Venugobal, J., Ramasamy, V.Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Applied Nanoscience. 2016; 6:399-408.

Gamboa, S.M., Rojas, E.R., Martínez, V.V., Vega-Baudrit, J. Synthesis and characterization of silver nanoparticlesand their application as an antibacterial agent. International Journal of Biosensors & Bioelectronics, 2019; 5(5):166-173.

Liao, C., Li, Y., Tjong, S.C. Bactericidal and cytotoxic properties of silver nanoparticles. International Journal of Molecular Sciences. 2019; 20: 449.

Dong, Y., Zhu, H., Shen, Y., Zhang, W., Zhang, L. Antibacterial activity of silver nanoparticles of different particle size against Vibrio natriegens. PLoS ONE. 2019;14(9): e0222322.

Salomoni, R., léo, P., Montemor, A.F., Rinaldi, B.G.,Rodrigues, M.F.A. Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa. Nanotechnology, Science and Applications. 2017;10: 115-121.

Raja, G., Jang, Y., Suh, J., Kim, H., Ahn, S.H., Kim, T. Microcellular environmental regulation of silver nanoparticles in cancer therapy: a critical review. Cancers. 2020; 12: 664.

Jabir, M.S., Nayef, U.M., Jawad, K.H., Taqi, Z.J., Hasoon, B.A., Ahmed, N.R. Porous silicon nanoparticles prepared via an improved method: A developing strategy for a successful antimicrobial agent against Escherichia coli and Staphylococcus aureus, Materials Science and Engineering. 2018; 454: 012077.

Pustovalova, M., Alhaddad, L., Smetanina, N., Chigasova, A.,Blokhina, T., Chuprov-Netochin, R., et al., The p53–53BP1-related survival of A549 and H1299 human lung cancer cells after multifractionated radiotherapy demonstrated different response to additional acute X-ray exposure. International Journal of Molecular Sciences. 2020; 21: 3342.

Kanwal, Z., Raza, M.A., Riaz, S., Manzoor, S., Tayyeb, A., Sajid, I.,et al.,Synthesis and characterization of silver nanoparticle-decorated cobalt nanocomposites ([email protected]) and their density-dependent antibacterial activity. Royal Society Open Science.2019;6:182135.

Xu, L., Wang, Y., Huang, J., Chen, C., Wang, Z., Xie, H. Silver nanoparticles: synthesis, medical applications and biosafety.Theranostics. 2020;10(20): 8996-9031.

Leersnyder, I.D., Rijckaert, H., Gelder, L.D., Driessche, I.V., Vermeir, P. High variability in silver particle characteristics, silver concentrations, and production batches of commercially available products indicates the need for a more rigorous approach. Nanomaterials. 2020; 10: 1394.

Zhang, X., Liu, Z., Shen, W., Gurunathan, S. Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. International Journal of Molecular Sciences.2016; 17: 1534.

Jayakar, V., Lokapur, V., Nityasree, B. R., Chalannavar, R. K., Lasrado, L. D., Shantaram, M. Optimization and green synthesis of zinc oxide nanoparticle using Garcinia cambogia leaf and evaluation of their antioxidant and anticancer property in kidney cancer (A498) cell lines. Biomedicine. 2021 Jul 7;41(2):206-222.

Kazemi, M., Akbari, A., Sabouri, Z., Soleimanpour, S., Zarrinfar, H., Khatami, M., et al.,Green synthesis of colloidal selenium nanoparticles in starch solutions and investigation of their photocatalytic, antimicrobial, and cytotoxicity effects. Bioprocess and Biosystems Engineering. 2021 Jun;44(6):1215-1225.

Barras, F., Aussel, L., Ezraty, B. Silver and antibiotic, new facts to an old story. Antibiotics.2018; 7: 79.

Dakal, T.C., Kumar, A., Majumdar, R.S., Yadav, V. Mechanistic basis of antimicrobial actions of silver nanoparticles. Frontiers in Microbiology. 2016; 7: 1831.

Morones, J.R., Elechiguerra, J.L., Camacho, A., Holt, K., Kouri, J.B., Ramírez, J.T., et al., The bactericidal effect of silver nanoparticles. Nanotechnology. 2005;16(10):2346-2353.

Ansari, M.A., Khan, H.M., Khan, A.A.,Malik, A.,Sultan, A.,Shahid, M., et al., Evaluation of antibacterial activity of silver nanoparticles against MSSAand MSRA on isolates from skin infections. Biology and Medicine. 2011; 3(2): 141-146.

Domínguez, A.V., Algaba, R.A., Canturri, A.M., Villodres, Á.R., Smani, Y. Antibacterial activity of colloidal silver againstGram-negative and Gram-positive bacteria. Antibiotics. 2020; 9: 36.

Kim, J.S., Kuk, E., Yu, K.N., Kim, J.H., Park, S.J., Lee, H.J.,et al., Antimicrobial effects of silver nanoparticles. Nanomedicine.2007; 3(1):95-101.

Feng, Q.L., Wu, J., Chen, G.Q., Cui, F.Z., Kim, T.N., Kim, J.O. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. Journal of Biomedical Materials Research. 2000; 52(4):662-668.

Ratan, Z.A., Haidere, M.F., Nurunnabi, Md., Shahriar, S.Md., Ahammad, A.J.S., Shim, Y.Y., et al., Green chemistry synthesis of silver nanoparticles and their potential anticancer effects. Cancers. 2020; 12: 855.

Yuan, Y., Zhang, S., Hwang, J., Kong, I. Silver nanoparticles potentiates cytotoxicity and apoptotic potential of camptothecin in human cervical cancer cells. Oxidative Medicine and Cellular Longevity. 2018; 2018: 6121328.

Aziz, N., Faraz, M., Sherwani, M.A., Fatma, T., Prasad, R. Illuminating the anti-cancerous efficacy of a new fungal chassis for silver nanoparticle synthesis. Frontiers in Chemistry.2019; 7: 65.

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Published

2021-09-07

How to Cite

1.
Abdulsahib SS. Synthesis, characterization and biomedical applications of silver nanoparticles. Biomedicine [Internet]. 2021Sep.7 [cited 2021Sep.22];41(2):458-64. Available from: https://biomedicineonline.org/index.php/home/article/view/1058