Synthesis, characterization and biomedical applications of silver nanoparticles
Keywords:Nanobiotechnology, antimicrobial, toxicity activity, tumor therapy
Introduction and Aim:Silver nanoparticles (AgNPs) have been extensively useful in biomedical applications.This study aims to synthesize silver nanoparticles by lasers ablation and to use them as an anti-bacterial and anti-cancer agent.
Materials and Methods:According to the current study, Ag-nanoparticles can be synthesized easily using pulsed laser ablation on a 99.81% pure silver target immersed in deionized water. The prepared nanoparticles werecharacteredbyUV-vis spectroscopy and X-ray diffraction (XRD), transmission electron microscopy (TEM), and atomic force microscopy (AFM) technique.Using a well-diffusion process, the anti-bacterial action of the synthesized Ag nanoparticles was tested versus two Gram-positive bacteria species (S. aureus and S. pyogenes) and two species of Gram-negative bacteria (E. coli and P. aeruginosa).The anticancer activity of the silver nanoparticles was evaluated by the MTT assay.
Results:The generated AgNps had a maximum absorbance peak of 400 nm. The XRD analysis verified that the synthesized silver nanoparticles had been nanocrystalline. The AgNPsdid not affect any of the blood parameters. Gram-negative bacteria are more affected by silver than Gram-positive bacteria. The Ag nanoparticles had been shown a maximum anti-bacterial action at a concentration of 80 µg/mland had a lower effect with 20 µg/ml concentration while their efficacy at 40 and 60 µg/ml concentrationsappeared to be variable against all bacterial species.The findings show that AgNPshavea cytotoxic influence on cancer cellsin 80 ?g/ml concentration.
Conclusion:In comparison to Gram-positive bacteria, silver nanoparticles show high antibacterial activity against Gram-negative bacteria. The prepared nanoparticles have a potent effect on cancer cells and restricted harmful effects on RBCs.
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