Evaluation of efficacy of different tooth paste formulations in reducing the oral microbial load - An in vivo study


  • G Priyal
  • Maji Jose
  • Shruti Nayak
  • Vidya Pai
  • Sudeendra Prabhu




Dentifrices, tooth pastes, herbal, non herbal, plaque, microflora, antimicrobial efficacy


Introduction and Aim: Toothpastes are the most common preventive means in oral health care. Many commercially available dentifrices claim to have antimicrobial properties, but little research has been conducted to investigate these claims. Therefore, this study was conducted to evaluate the efficacy of different toothpaste formulations in reducing the oral microbial load.

Methodology: A total of six types of tooth pastes-4 herbal and 2 non-herbal- were assigned to a group of 20 subjects each, who used it for a period of six weeks. During the course of the study, at the end of 1st, 3rd and 6th week, subgingival plaque samples collected were subjected to microbial analysis.

Results: Microbial analysis identified colonies of organisms such as S. mitis, S. mutans, S. salivarius, Peptostreptococcus species, Prevotella species and Lactobacillus species. Colony forming units (cfu) calculated indicated a reduction in microbial load from 1st to 6th week of use of different tooth paste formulations. However statistical analysis of the results did not show significant differences between the groups (p>0.005).

Conclusion: The selected tooth paste formulations were effective in controlling the microbial load and therefore contributing to maintain good oral hygiene. However, practicing appropriate oral hygiene measures & brushing technique is of utmost importance in maintaining good oral health than the effectiveness of various ingredients in the toothpastes used.

Author Biographies

G Priyal

AJ Shetty Institute of Dental Sciences, Mangalore, Karnataka, India

Maji Jose

Department of Oral Pathology, Centre for Forensic Odontology, Yenepoya Dental College, Yenepoya Deemed to be University, Mangalore, Karnataka, India

Shruti Nayak

Department of Oral Pathology,  Yenepoya Medical College, Yenepoya Deemed to be University, Mangalore, Karnataka, India

Vidya Pai

Department of Microbiology, Yenepoya Medical College, Yenepoya Deemed to be University, Mangalore, Karnataka, India

Sudeendra Prabhu

Centre for Forensic Odontology, Yenepoya Medical College, Yenepoya Deemed to be University, Mangalore, Karnataka, India


Oral health- Global Health Testimony for the 2005 Global Health Summit Philadelphia, Pennsylvania June 5, 2005.

Global Review on Oral Health in Ageing Societies WHO Kobe Centre for Health Development Ageing and Health Technical Report. 2002; (3).

Hatti, S., Ravindra, S., Sathpathy, A., Kulkarni, R. D., Parande, M. V. Biofilm inhibition and antimicrobial activity of a dentifrice containing salivary substitutes. Int J Dent Hygiene 2007; 5: 218-224.

Harris, N., Franklin, G. G. Primary preventive dentistry. 6th edition Pearson prentice hall upper saddle River, New Jersey.

Todar, K. The bacterial flora of humans. Todar’s online textbook of bacteriology 2007.

Abdel Rahman, H. F., Skaug, N., Francis, G. W. In vitro antimicrobial effects of crude miswak extracts on oral pathogens. Saudi Dental Journal. 2002; 1(14).

Almas, K., AL-Zeid, Z. The immediate antimicrobial effect of a tooth brush and miswak on cariogenic bacteria: a clinical study. J Cotemp Dent Pract. 2004; 5: 105-114.

Patil, S., Venkataraghavan, K., Anantharaj, A., Patil, S. Comparison of two commercially available toothpastes on the salivary streptococcus mutans count in urban preschool children -an in vivo study. International dentistry SA; 12(4): 72-78.

Jose, S., Beegum, G. R. J. In vitro susceptibility of viridans streptococci to leaf extracts of Mangifera indica. Indian Journal of Microbiology. 2007; 47: 160-163.

Prashant, G. M., Chandu, G. N., Murulikrishna, K. S., Shafiulla, M. D. The effect of mango and neem extrct on four organisms causing dental caries: streptococcus mutans, streptococcus salivarius, streptococcus mitis and streptococcus sanguis: An in vivo study. Indian J Dent Res 2007; 18(4): 148-151.

Kanwal, Q., Hussain, I., Siddiqui, H, L., Javaid, A. Antifungal activity of flavonoids isolated from mango (Mangifera indica l) leaves. Natural Product Research. 2010; 24(20): 1907-1914.

Gupta, P., Agarwal, N., Manjunath, B. C., Bhalla, A. Evaluating the anti-plaque efficacy of meswak containing dentifrices: a triple blind controlled trial. J Pharm Bioallied Sci. 2012; 4(4): 282-285.

Kumar, D. N., Sidhu, P. M. The antimicrobial activity of azardirachta indica, glycyrrhiza glabra, cinnamum zeylanicum, Syzygium aromaticum, Accacia nilotica on streptococcus mutans and enterococcus faecalis - An in vitro study. Endontology. 2011; 1(23): 101-104.

Kumar, M. K. P., Priya, N. K., Madhushankari, G. S. Anti-cariogenic efficacy of herbal and conventional toothpastes - a comparative in-vitro study. Journal of International Oral Health. Mar-Apr 2013; 5(2):8-13.

Takarada, K., Kimizuka, R., Takahashi, N., Honma, K., Okuda, K., Kato, T. A comparison of the antibacterial efficacies of essential oils against oral pathogens. Oral Microbiol Immunol. 2004: 19; 61-64.

Ahmad, I., Mehmood, Z., Mohammad, F. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol. 1998 Sep; 62(2): 183-193.

Firas, A., Bayati, A., Khudir, D., Sulaiman. In vitro antimicrobial activity of Salvadora persica L. extracts against some isolated oral pathogenssss in Iraq, Turk J Biol. 2008; 32: 57-62.

Cowan, M. M. Plant products as antimicrobial agent. Clin. Microbiol. Rev. 1999; 12(4): 564.

Buzalaf, M. A., Pessan, J. P., Honório, H. M., Ten Cate, J. M. Mechanisms of action of fluoride for caries control. Monogr Oral Sci. 2011; 22: 97-114.

Perlich, M. A., Bacca, L. A., Bollmer, B. W., Lanzalaco, A. C., McClanahan, S. F., Sewak, L. K. The clinical effect of a stabilized stannous fluoride dentifrice on plaque formation, gingivitis and gingival bleeding: a six-month study. The Journal of Clinical Dentistry. 1995; 6: 54-58.

Severi, J. A., Lima, Z. P., Kushima, H., Brito, A. R. M. S., Santos, L.C., Vilegas, W. Polyphenols with Antiulcerogenic Action from Aqueous Decoction of Mango Leaves (Mangifera indica L.). Molecules. 2009;14: 1098-1110.

Nevitt, G. A., Witter, D. H., Bowman, W. D. Topical applications of sodium fluoride and stannous fluoride. Public Health Rep. 1958; 73(9): 847-850.

Sumanth, G., Beena, G., Bhongade, L. Oral health status of young adults using indigenous oral hygiene methods. Stomatologica India. 1992; 5: 17-23.




How to Cite

Priyal G, Jose M, Nayak S, Pai V, Prabhu S. Evaluation of efficacy of different tooth paste formulations in reducing the oral microbial load - An in vivo study. Biomedicine [Internet]. 2021Sep.7 [cited 2021Sep.22];41(2):465-71. Available from: https://biomedicineonline.org/index.php/home/article/view/1059

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