Estimation of genotype MTBDRPLUS line probe assay in detection of rifampicin and isoniazid resistance in comparison to liquid culture (BACTEC-960) drug susceptibility testing in a tertiary care hospital from Eastern India

Authors

  • Chandan Kumar Poddar
  • Namrata Kumari
  • Rakesh Kumar
  • Shivendra Kumar Shahi
  • Naresh Kumar
  • Shailesh Kumar

DOI:

https://doi.org/10.51248/.v41i2.1060

Keywords:

GenoType MTBDRplus test, BACTEC MGIT 960, MDR-TB, Rifampicin (RIF), Isoniazid (INH)

Abstract

Introduction and Aim: India has the uppermost trouble of Multidrug resistant tuberculosis (MDR-TB) is a major challenge controlling resistance, reducing transmission and improving handling outcomes in MDR-TB patients is dependent on susceptibility testing. Isoniazid (INH) and rifampicin (Rif) are the key first-line antituberculosis drugs, and resistance to these drugs i.e., MDR-TB, is likely to result in treatment failure and poor clinical outcomes. The present study was done to compare the performance of line probe assay test (GenoType® MTBDRplus) with liquid culture (MGIT 960) system for the detection of resistance to first-line drugs.

 

Materials and Methods: We estimate the performance of LPAs to BACTEC MGIT 960 system for susceptibility testing of bacterial resistance to first-line drugs: rifampicin (RIF), isoniazid (INH).

 

Results: We performing Drug susceptibility testing (DST), 219/258 MTB cultures were viable after subculture the results of DST using the MGIT 960 system were compared to those obtained by line probe assay. LPA detected a total 46/258 (17.81%) samples as drug resistant, of which 35/258 (13.70%) were resistant to both rifampicin and isoniazid (MDR), 6/258 (2.28%) were rifampicin mono?resistant samples and 11/258 (4.11%) were isoniazid mono?resistant. Out of the culture?positive samples (219), LPA detected 39/219 (17.83%) as drug?resistant, of which 31/219 (14.2%) were resistant to both rifampicin and isoniazid, 5/193 (2.08%) were rifampicin mono?resistant and 8/219 (3.7%) were isoniazid mono?resistant.

Conclusion: Drug resistant TB poses an enormous threat to TB control programs worldwide. Effective treatment of MDR-TB is very expensive, particularly in middle income countries such as India.

Author Biographies

Chandan Kumar Poddar

Department of Microbiology, Indira Gandhi Institute of Medical Sciences, Patna, Bihar, India

Namrata Kumari

Department of Microbiology,  Indira Gandhi Institute of Medical Sciences, Patna, Bihar, India

Rakesh Kumar

Department of Microbiology, Indira Gandhi Institute of Medical Sciences, Patna, Bihar, India

Shivendra Kumar Shahi

Department of Microbiology, Indira Gandhi Institute of Medical Sciences, Patna, Bihar, India

Naresh Kumar

Department of Medicine, Indira Gandhi Institute of Medical Sciences, Patna, Bihar, India

Shailesh Kumar

Department of Medicine, Indira Gandhi Institute of Medical Sciences, Patna, Bihar, India

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Published

2021-09-07

How to Cite

1.
Poddar CK, Kumari N, Kumar R, Shahi SK, Kumar N, Kumar S. Estimation of genotype MTBDRPLUS line probe assay in detection of rifampicin and isoniazid resistance in comparison to liquid culture (BACTEC-960) drug susceptibility testing in a tertiary care hospital from Eastern India. Biomedicine [Internet]. 2021Sep.7 [cited 2021Sep.22];41(2):472-6. Available from: https://biomedicineonline.org/index.php/home/article/view/1060