The diagnostic value of urinary culture filtrate protein-10antigen in childhood tuberculosis

Authors

  • Agustin Iskandar
  • Maimun Z Arthamin
  • Gilang Kusdinar
  • Kusworini Handono
  • Ery Olivianto
  • Aryati Aryati
  • Andrea Aprilia

DOI:

https://doi.org/10.51248/.v41i2.1062

Keywords:

childhood TB, biomarker, urinaryCFP-10

Abstract

Introduction and Aim:Childhood tuberculosis (TB) remains a major problem worldwide.However, diagnosis of tuberculosis in children is often complicated by the difficulty in obtaining a proper sputum specimens and low sensitivity of the gold standard diagnostic test to confirm the presence of Mycobacterium tuberculosis(M.tb)in this age group. Recently, M.tbantigen detection in urinaryspecimenshas become a popular method. It is non-invasiveand handling of specimen is simple. It was reported that urinary CFP-10, a specific protein of M.tb, has emerged as a potential biomarker in the future. However, its diagnostic value as a new biomarker in childhood TB remains poorly understood.The aim of the study is to determine the diagnostic value of urinary CFP-10 in childhood TB.

 

Methods: Seventy children with suspected pulmonary or extrapulmonary TB were enrolled. Tuberculosis was diagnosed by performingTuberculin skin test, chest x-ray, microscopic examination, and microbiological cultureobtainedfrom sputum or gastric lavage specimen. The level ofurinary CFP-10 antigen was analyzedbyELISA (Elabscience, China). Statistical analyseswereperformed using SPSS 21.0 and p-values of <0.05 were consideredstatistically significant.

 

Results: The levels of urinary CFP-10 in subjects diagnosed with TB was higher than that of the non-TB subjects, 4.13(0.62) vs 0.43(0.14) pg/mL, p=0.005. The cut-off value forurinary CFP-10 level reached 0.39 pg/mL (sensitivity 65% and specificity 67%). This value became0.54 pg/mL (sensitivity 61% and specificity 62%)in microbiologically confirmed cases.

 

Conclusion: The urinary CFP-10 level has moderate diagnostic value for diagnosing childhood TB.

 

 

Author Biographies

Agustin Iskandar

Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya/ Saiful Anwar General Hospital, Malang, East Java, Indonesia

Maimun Z Arthamin

Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya/ Saiful Anwar General Hospital, Malang, East Java, Indonesia

Gilang Kusdinar

Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya/ Saiful Anwar General Hospital, Malang, East Java, Indonesia

Kusworini Handono

Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya/ Saiful Anwar General Hospital, Malang, East Java, Indonesia

Ery Olivianto

Department of Pediatric, Faculty of Medicine, Universitas Brawijaya/ Saiful Anwar General Hospital, Malang, East Java, Indonesia

Aryati Aryati

Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga/ dr.Soetomo General Hospital, Surabaya, East Java, Indonesia

Andrea Aprilia

Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya/ Saiful Anwar General Hospital, Malang, East Java, Indonesia

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Published

2021-09-07

How to Cite

1.
Iskandar A, Arthamin MZ, Kusdinar G, Handono K, Olivianto E, Aryati A, Aprilia A. The diagnostic value of urinary culture filtrate protein-10antigen in childhood tuberculosis. Biomedicine [Internet]. 2021Sep.7 [cited 2021Sep.22];41(2):477-81. Available from: https://biomedicineonline.org/index.php/home/article/view/1062