Phagocytic and oxidative burst activity of neutrophils in type 2 diabetic patients with foot ulcers


  • Grisilda Vidya Bernhardt Department of Biochemistry, RAK College of Medical Sciences , RAK Medical and Health Science University, RAK , UAE
  • Pooja Shivappa Department of Basic Sciences, RAK College of Medical Sciences , RAK Medical and Health Science University, RAK , UAE
  • Manjula Shantaram Department of Biochemistry, Mangalore University , Jnana Kaveri PG Centre, Chikka Aluvara, 571232, Karnataka, India
  • Varsha Jayakar Department of Biochemistry, Mangalore University , Jnana Kaveri PG Centre, Chikka Aluvara, 571232, Karnataka, India
  • Vinayak Lokapur Department of Biochemistry, Mangalore University , Jnana Kaveri PG Centre, Chikka Aluvara, 571232, Karnataka, India
  • Janita R. T. Pinto Department of Biomedical Sciences, College of Medicine, Gulf Medical University, Ajman, UAE



Neutrophil phagocytic index, respiratory burst, type 2 DM patients, diabetes, foot ulcers, predictors for treatment efficacy of foot ulcers


Introduction and Aim: Diabetic foot ulcers are common complications seen in diabetic patients. Treatment of this  disabling foot sore remains a challenge to health care professionals. This study aimed at evaluating whether the neutrophils from type 2 diabetic patients with foot ulcers present an impairment of phagocytic index and impairment in respiratory burst. We also aimed at understanding whether the impairment in neutrophil phagocytic activity can be alleviated with short course of standard treatment regime for foot ulcers.


Methodology: For this case-controled study, 43 participants  with type 2 diabetes  (18  with foot ulcers and 25 without foot ulcers) were prospectively recruited along with 18 healthy volunteers. Phagocytic activity of neutrophils and respiratory burst of neutrophils was assessed  along with ESR, percentage neutrophil counts before and after 2 weeks of standard treatment for foot ulcers.


Results: Neutrophils of type 2 diabetic patients (with and without foot ulcers ) showed  lower levels of phagocytic index and lower percentage of respiratory burst  on  comparison with non-diabetic subjects. Furthermore, on receiving treatment for foot ulcers, a significant improvement in neutrophil phagocytic indices were observed, along with improvement in wound ulcer score.


Conclusion: Phagocytic activity of the neutrophils is impaired  in  type 2 diabetics (with and without foot ulcers). Neutrophil phagocytic indices can be improved on glycemic control. Additionally, improvement in neutrophil phagocytic indices after short course treatment for foot ulcers can be useful markers to predict treatment efficacy and in prognosis of diabetic foot ulcers.


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How to Cite

Bernhardt GV, Shivappa P, Shantaram M, Jayakar V, Lokapur V, Pinto JRT. Phagocytic and oxidative burst activity of neutrophils in type 2 diabetic patients with foot ulcers. Biomedicine [Internet]. 2021Dec.31 [cited 2022Jan.20];41(4):776-80. Available from:



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