A biotechnological approach to optimization and production of melanin by Brevibacillus invocatus strain IBA, under submerged fermentation

Authors

  • Avinash Irappa Ammanagi
  • Shivasharana C.T.
  • Krishnaveni R.
  • Abhijeeth Shivappa Badiger
  • Vijaya Kumar Ramaraj
  • Srinath B. S.
  • Yalpi Karthik

DOI:

https://doi.org/10.51248/.v42i2.1315

Keywords:

Brevibacillus invicatus, tyrosinase activity, molecular sequencing, submerged fermentation, chromatographic technique

Abstract

Introduction and Aim: Melanin is a macromolecule with many biological activities, found across the animal, plant and microbes. This study focuses on parametric optimization of melanin production using a microbial.

 

Materials and Methods: The soil samples were diluted to tenfold and spread plate technique was employed. The isolated cultures were subjected for melanin production the potential strain was selected and employed for mass production using nitrate broth supplement with L- tyrosine. The culture identification is by biochemical, microscopic and molecular sequencing and data retrieved through NCBI. Different physical parameters are used for the optimum growth of isolated culture.

 

Results: The potential microbial culture was identified through 16s rRNA was Brevibacillus invocatus strain IBA with accession number 696201.1. This is an effective culture produced tyrosinase activity was 3282 U mg-1 and 0.554 g l-1 cell mass. The incubation duration highest melanin production was 48 hours, with 0.328 g l-1. Melanin production was better with 0.352 g l-1 at 120rpm agitation. In pH 6.5 remained establish to be optimum with 0.47 g l-1. Optimum temperature at 35°C high yield with 0.487 g l-1. Sucrose remained establish to be best carbon source with 0.512 g l-1 of melanin; while Tryptone as nitrogen sources produced the maximum melanin 0.548 g l-1. Thin layer chromatography was one of the useful methods to detect melanin from crude to pure melanin had an Rf value of 0.62.

 

Conclusion: Chemical synthesis of melanin is expensive and involves multiple steps, animal or plant sources are cumbersome process. There are limitation and challenges to use the animal origin melanin for therapeutics and for food process. This investigation provides knowledge on factors affecting the melanin production using a bacterial culture in submerged fermentation technique.

Author Biographies

Avinash Irappa Ammanagi

Department of Studies in Biotechnology and Microbiology, Karnataka University, Dharwad, 580003, Karnataka, India

Shivasharana C.T.

Department of Studies in Biotechnology and Microbiology, Karnataka University, Dharwad, 580003, Karnataka, India

Krishnaveni R.

Department of Microbiology Vijayanagara Sri Krishnadevaraya University, Ballari, 583 105, Karnataka, India

Abhijeeth Shivappa Badiger

Department of Biotechnology, Sri Dharmasthala Manjunatheshwara College, Ujire, 574240, Karnataka, India

Vijaya Kumar Ramaraj

Shilpa Biologicals Private Limited, Dharwad, 580 011, Karnataka, India

Srinath B. S.

Department of Studies and Research in Microbiology, Mangalore University, Jnana Kaveri PG Centre, Chikka Aluvara, 571 232, Kodagu, Karnataka, India

Yalpi Karthik

Department of Studies and Research in Microbiology, Mangalore University, Jnana Kaveri PG Centre, Chikka Aluvara, 571 232, Kodagu, Karnataka, India

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Ammanagi, A., Shivasharana C.T., Krishnaveni R., Badiger, A., Ramaraj, V. Functional and structural characterization of melanin from Brevibacillus invocatus strain IBA. Docklady Biological Sciences, 2021; Vol. 500, pp 159-169. © Pleiades publishing, Ltd.

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Published

2022-05-01

How to Cite

1.
Ammanagi AI, C.T. S, R. K, Badiger AS, Ramaraj VK, B. S. S, Karthik Y. A biotechnological approach to optimization and production of melanin by Brevibacillus invocatus strain IBA, under submerged fermentation. Biomedicine [Internet]. 2022 May 1 [cited 2022 Nov. 27];42(2):318-24. Available from: https://biomedicineonline.org/home/article/view/1315

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