Evaluation of in vitro antioxidant, anti-inflammatory, anticoagulant and antiplatelet potential of Rhus mysorensis

Authors

  • Ghouseul Azam Department of Biochemistry, Kuvempu University, Jnanasahyadri, Shankaraghatta 577 451, Shivamogga, Karnataka, India
  • Sathisha G. Jayanna Department of Biochemistry, Kuvempu University, Jnanasahyadri, Shankaraghatta 577 451, Shivamogga, Karnataka, India
  • Anitha Nelliankla Department of Biochemistry, Kuvempu University, Jnanasahyadri, Shankaraghatta 577 451, Shivamogga, Karnataka, India
  • Vasanthraj Boraiah Department of Biochemistry, Kuvempu University, Jnanasahyadri, Shankaraghatta 577 451, Shivamogga, Karnataka, India
  • Sujatha M. Hanumegowda Department of Biochemistry, Kuvempu University, Jnanasahyadri, Shankaraghatta 577 451, Shivamogga, Karnataka, India
  • Devaraja Sannaningaiah Department of Studies and Research in Biochemistry and Centre for Bioscience and Innovation, Tumkur University, Tumkur-572103, Karnataka, India
  • Poornima D. Vijendra Department of Biochemistry, Davanagere University, Shivagangothri, Davanagere - 577 002, Karnataka, India
  • Vadlapudi Kumar Department of Biochemistry, Davanagere University, Shivagangothri, Davanagere - 577 002, Karnataka, India
  • Riaz Mahmood Department of Biotechnology, Kuvempu University, Jnanasahyadri, Shankaraghatta 577 451, Shivamogga, Karnataka, India

DOI:

https://doi.org/10.51248/.v41i4.1365

Keywords:

<i>Rhus mysorensis</i>, antioxidant, anti-inflammatory, anticoagulant, antiplatelet, liquid-liquid partition

Abstract

Since ancient times human beings are using plant-based medicines for the treatment of various ailments, especially in the rural areas, due to their availability and affordability. Rhus mysorensis (RM) is widely used as a traditional medicine to treat various ailments. Owing to its potential medicinal value, the present study was designed to explore the in vitro antioxidant, anti-inflammatory, anticoagulant and antiplatelet properties of purified column fraction of RM.

The methanol extract of stem bark powder was sequentially fractioned by solvent partitioning. The liquid methanol fraction was further fractionated by column chromatography using gradient elution. Eluted fractions were analyzed using HPLC for percentage purity and yield. The fraction with higher percentage of purity and yield was assessed for in vitro antioxidant activity by measuring SOD and GPx activities, anti-inflammatory activity by the inhibition of nitric oxide (NO) production in LPS induced RAW264.7 cells, anticoagulation by plasma recalcification time and antiplatelet activity by agonists induced platelet aggregation respectively.

The antioxidant potency of column fraction (B8) revealed that, highest enzyme activities were recorded at a concentration of 320µg/ml. The enzyme activity was found to be 2.45 U/ml for SOD and 135.75 U/L for GPx respectively. Purified column fraction (B8) of RM significantly reduces the production of NO in LPS stimulated RAW 264.7 cell lines at 320????g/ml concentration with 31.90% of inhibition. The anticoagulant activity of purified fraction was determined in terms of plasma recalcification time. Interestingly, the fraction showed the most potent anticoagulant activity both in PRP and PPP as it prolonged the clotting time.

The findings indicate that the stem bark of RM possesses potent antioxidant, anti-inflammatory, anticoagulant and antiplatelet activities, supporting the use of this species for treating oxidative stress-induced inflammatory diseases. Further, bioactivity guided fractionation studies to characterize and identify specific phytochemicals responsible for these biological activities are needed.

References

Liguori, I., Russo, G., Curcio, F., Bulli, G., Aran, L., Della-Morte, D., et al., Oxidative stress, aging, and diseases. Clinical interventions in aging. 2018; 13: 757.

Rivera, J. O., Loya, A. M., Ceballos, R. Use of herbal medicines and implications for conventional drug therapy medical sciences. Altern Integ Med. 2013: 2(6): 1-6.

Sagin, F. G., Sozmen, E. Y. Anti-inflammatory effects of dietary antioxidants. Current Medicinal Chemistry-Anti-Inflammatory & Anti-Allergy Agents. 2004; 3(1): 19-30.

Sostres, C., Gargallo, C. J., Arroyo, M. T., Lanas, A. Adverse effects of non-steroidal anti-inflammatory drugs (NSAIDs, aspirin and coxibs) on upper gastrointestinal tract.Best practice & research Clinical gastroenterology. 2010; 24(2): 121-132.

Ofori–Baah, S., Borquaye, L. S. Ethanolic leaf extract from Strophanthus gratus (Hook.) Franch (Apocynaceae) exhibits anti-inflammatory and antioxidant activities. Cogent Biology. 2019; 5(1): 1710431.

Ravipati, A. S., Zhang, L., Koyyalamudi, S. R., Jeong, S. C., Reddy, N., Bartlett, J., et al., Antioxidant and anti-inflammatory activities of selected Chinese medicinal plants and their relation with antioxidant content. BMC Complementary and Alternative Medicine. 2012; 12(1): 1-14.

Arulselvan, P., Fard, M. T., Tan, W. S., Gothai, S., Fakurazi, S., Norhaizan, M. E., et al., Role of antioxidants and natural products in inflammation. Oxidative medicine and cellular longevity, 2016.

Buch, M. H., Prendergast, B. D., Storey, R. F. Antiplatelet therapy and vascular disease: an update. Therapeutic advances in cardiovascular disease. 2010; 4(4): 249-275.

Huber, K., Bates, E. R., Valgimigli, M., Wallentin, L., Kristensen, S. D., Anderson, J. L., et al., Anti-platelet and anticoagulation agents in acute coronary syndromes: what is the current status and what does the future hold? American Heart Journal. 2014; 168(5): 611-621.

Fuentes, E., Palomo, I. Antiplatelet effects of natural bioactive compounds by multiple targets: Food and drug interactions. Journal of Functional Foods. 2014; 6: 73-81.

Bienvenu, E., Priti, M. D., Yadav, S. R. Medicinal plants of Southwestern Maharashtra. In: Pullaiah T (Ed), Biodiversity in India 4(1). New Delhi: Regency Publications. 2006; 180-181.

Subbaiah, K. V., Savithramma, N. Bioprospecting and documentation of traditional medicinal plants used to treat leprosy and eczema by ethnic groups of Kurnool district, Andhra Pradesh. India. J Pharm Res. 2011: 4: 4488-4490.

Aman, M., Ravishankar Rai, V.Samaga, P. V., Antimicrobial and phytochemical screening of Boswellia serrata Roxb., Rhus mysorensis Heyne, Strychnospotatorum Linn. F. and Schefflera stellata Gaertn. Medicinal and Aromatic Plant Science and Biotechnology. 2010; 4(1): 69-72.

Renuka rani, G., SingaraCharya, M. A., Viswanadham, M., Thupurani, M. K. Anti-inflammatory activity of flavonoids isolated from Rhus Mysorensis. IOSR Journal of Pharmacy and Biological Sciences. 2017; 12: 37-40.

Renuka rani, G., SingaraCharya, M. A., Viswanadham, M., Thupurani, M. K. Antidiabetic activity of the compounds isolated from Rhus mysorensis Plant Extract. IOSR Journal of Biotechnology and Biochemistry. 2017; 3: 37-42.

Venkataiah, G. P., Humeranaaz, S. Evaluation of anti-anxiety activity of ethanolic extract of Rhus mysorensis on wistar rats. Int. J. of Pharmacology and Clin, Res. 2017; 1(2): 2531.

Dudekula, N. K., Duza, M. B., Janardhan, N., Duraivel, S., 2014. Evaluation of the hepatoprotective activity of Rhus mysorensis in albino rats. Indian Journal of Research in Pharmacy and Biotechnology. 2014; 2(1): 1010.

Sudheshna, L., Sukesh Krishna, C. L., Srinivasa Rao, A. Anti-Urolithiatic Activity of Rhus Mysorensis against Experimentally Induced Urolithiasis in Male Albino Rats. Journal of Medical Science and Clinical Research. 2015; (3): 7546-7551.

Bhavya, E., Gunasekaran, V., Jayabalan, G. Phytochemical screening and evaluation of antifertility activities of Rhus mysorensis in female albino rats. Drug Discovery. 2008; (12): 38-41.

Sanwar, M. L., Sulakhiya, K., Kumar, P. Anti-diabetic, hypolipidemic and antioxidant activities of hydroethanolic root extract of Rhus mysorensis Heyne in Streptozotocin Induced Diabetes in Wistar Male Rats. Phcog J. 2014; 6(3): 62-71.

Beauchamp, C., Fridovich, I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry. 1971; 44(1):276-287.

Flohé, L., Günzler, W. A. Assays of glutathione peroxidase. Methods in Enzymology. 1984; 105: 114-120.

Quick, A.J.A study of the coagulation defect in hemophilia and in jaundice. Am. J. Med. Sci. 1935; 190: 501-511.

Ardlie, N. G., Han, P., Enzymatic basis for platelet aggregation and release: the significance of the ‘platelet atmosphere’ and the relationship between platelet function and blood coagulation. British journal of haematology. 1974; 26(3): 331-356.

Born, G.V.R., Cross, M. The aggregation of blood platelets. The Journal of physiology. 1963; 168(1): 178-195.

Sudipta, K. M., Kumara Swamy, M., Balasubramanya, S. Anuradha, M. Assessment of genetic fidelity, antioxidant enzyme activity and proline content of micropropagated and field grown plants of Leptadenia reticulata (wight &arn.)-an endangered medicinal plant. Plant Cell Biotechnol Mol Biol. 2014; 15(3&4): 127-135.

Gadamsetty, G., Maru, S., Sarada, N. C. Antioxidant and anti-inflammatory activities of the methanolic leaf extract of traditionally used medicinal plant Mimusopselengi L. Journal of Pharmaceutical Sciences and Research. 2013; 5(6): 125.

Downloads

Published

2021-12-31

How to Cite

1.
Azam G, Jayanna SG, Nelliankla A, Boraiah V, Hanumegowda SM, Sannaningaiah D, Vijendra PD, Kumar V, Mahmood R. Evaluation of in vitro antioxidant, anti-inflammatory, anticoagulant and antiplatelet potential of Rhus mysorensis. Biomedicine [Internet]. 2021Dec.31 [cited 2022Jan.20];41(4):724-31. Available from: https://biomedicineonline.org/index.php/home/article/view/1365

Issue

Section

Original Research Articles

Most read articles by the same author(s)