Evaluation of Semecarpus kurzii Engler from Bay Islands for oxidative DNA damage protective activity and in vitro antioxidant potential


  • Sonali Das
  • Raghavendra L. S. Hallur
  • Asit Baran Mandal




Bay Islands, antioxidant, DNA damage assay, ethnomedicine, Semecarpus kurzii


Introduction and Aim: Harnessing traditional knowledge of medicinal plants is important for the betterment of mankind. We must safeguard traditional knowledge from misuse by miscreants living in modern societies and knowledge must be available in the public domain for use in drug designing in the healthcare system. Since the beginning of recorded history, Indians have used plants as a source of medicine, and their cultural past is incredibly rich. This study was conducted to evaluate the oxidative stress-induced DNA damage prevention and antioxidant potential of Semecarpus kurzii found in the Bay Islands.


Materials and Methods: The scavenging of superoxide, hydroxyl radicals, nitric oxide (NO), 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) radicals, ferric reducing power (FRAP), and lipid peroxidation inhibition activity were tested to determine antioxidant activity. DNA damage inhibition test was used to evaluate the protection against oxidative DNA damage.


Results: The S. kurzii extract showed dose-dependent scavenging of DPPH, superoxide anion, nitric oxide, hydroxyl radical and reducing power evaluated by comparing with standard antioxidants (ascorbic acid, alpha-tocopherol and BHT). However, the nitric oxide scavenging (IC50 =186.47) and superoxide scavenging (IC50 = 678.32) was more than that of DPPH (IC50 =28.03) and Hydroxyl radical (IC50 =89.10) scavenging capacity. In addition, S. kurzii extract and ascorbic acid showed lipid peroxidation inhibition activity. The extract also exhibited DNA protection which was confirmed by the DNA damage inhibition assay.


Conclusion: Our results corroborated that, the methanolic extract of S. kurzii bark has substantial antioxidant activity and DNA damage inhibition. The potential antioxidant and oxidative DNA damage preventive activity could be due to the existence of polyphenolic compounds in the S. kurzii bark.

Author Biographies

Sonali Das

Centre for Biotechnology, Pravara Institute of Medical Sciences-DU, Loni (Bk), 413736, Taluk Rahata, District Ahmednagar, Maharashtra,India

Raghavendra L. S. Hallur

Centre for Biotechnology, Pravara Institute of Medical Sciences-DU, Loni (Bk), 413736, Taluk Rahata, District Ahmednagar, Maharashtra,India

Asit Baran Mandal

Division of Crop Improvement, Central Research Institute for Jute & Allied Fibres (ICAR), Barrackpore, Kolkata, 700 120, West Bengal, India


Bhargava, N. Ethnobotanical studies of the tribes of Andaman and Nicobar Islands, India. I. Onge. Econ Bot. 1983; 37(1):110-119.

Chattopadhyay, D., Maiti, K., Kundu, A.P., Chakrabarty, M.S., Bhadra, R., Mandal, S.C., et al., Antimicrobial activity of Alstonia macrophylla: A folklore of Bay Islands. J Ethnopharmacol. 2001; 71(1): 49-55.

Chattopadhyay, D., Arunachalam, G., Mandal, A.B., Bhadra, R., Mandal, S.C. CNS activity of Mallotus peltatus Muell Arg. Leaf extracts: An ethnomedicine of Bay Islands. J Ethnopharmacol. 2003; 85(1): 99-105.

Dagar, H.S., Dagar, J.C. Plant folk medicines among the Nicobarese of Katchal Islands. India. Econ Bot. 1991; 45:114-119.

Chakraborty, T., Vasudeva Rao, M.K. Ethnobotanical studies of the shompen of Great Nicobar Islands. J Eco Taxon Bot. 1988; 12: 43-54.

Polterait, O., Antioxidants and free-radical scavengers of natural origin. Curr Org Chem. 1997;1: 415-440.

Gordon, M.C., David, J.N. Natural product drug discovery in the next millennium. Pharm Biol. 2001; 39:8-17.

Wink, D.A., Feelisch, M., Vodovotz, Y., Fukuto, J., Grisham, M.B. The chemical biology of nitric oxide. In: Reactive Oxygen Species in Biological Systems. Gilbert, D. L. and Cotton, C. A. (Eds.), Kluwer Academic/Plenum Publishers, New York. 1999; 245-291.

Devasagayam, T.P.A., Tilak, J.C., Boloor, K.K. Free radicals and antioxidants in human health: Current status and future prospects. J Assoc Physicians India. 2004; 52:794-804.

Cross, C.E.B., Halliwell, E.T., Borish, W.A., Pryor, B.N., Ames, R.L., Saul, J.M. McCord, J.M., Harman, D. Oxygen radicals and human disease. Ann Intern Med. 1987; 107: 526-545.

Zheng, W., Wang, S.Y. Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food Chemistry. 2001; 49:5165-5170.

Farooqi, J.A., Jamal, S., Ahmad, I. Isoricinoleic acid in Semecarpus kurzii Seed Oil. J. American Oil Chemist Soc. 1985; 62(12):1702-1703.

Das, S., Sheeja,T.E., Mandal, A.B. Ethnomedicinal uses of certain plants from Bay Islands. Indian J Trad Know. 2006; 5(2):207-211.

Jayakar, V., Lokapur, V., Shantaram, M. Identification of the volatile bioactive compounds by GC-MS analysis from the leaf extracts of Garcinia cambogia and Garcinia indica. Medicinal Plants-International Journal of Phytomedicines and Related Industries. 2020;12(4):580-590.

Lokapur, V., Jayakar, V., Shantaram, M. Phytochemical investigation, chemical composition, and in vitro antioxidant activities of various crude extracts of Holigarna ferrugenia Marchand. Medicinal Plants-International Journal of Phytomedicines and Related Industries. 2022;14(1):72-83.

Lokapur, V., Jayakar, V., Shantaram, M. Preliminary phytochemical screening, physicochemical analysis and in-vitro antioxidant activity of selected Holigarna species-Endemic plant species of Western Ghats. Biomedicine. 2020;40(4):460-466.

Lokapur, V., Jayakar, V., Divakar, M.S., Chalannavar, R.K., Lasrado, L., Shantaram, M. ZnO nanoparticles with spectroscopically controlled morphology, bioinspired from Holigarna grahamii (Wight) Kurz and delving its antioxidant and anticancer potential on A498 cell line. Materials Today Communications. 2022 Jun 1;31:103338.

Jose, M., Varghese, V.I., Jayakar, V., Lokapur, V., Srinivasa, K., Shantaram, M. Evaluation of antioxidant activities and free radical scavenging properties in mango leaves, husks of areca and coconut. Research Journal of Pharmaceutical Biological and Chemical Sciences. 2018 Jul 1;9(4):1607-1619.

Nishimiki, M., Rao, N.A., Yagi, K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochemistry and Biophysics Research Communication. 1972; 46(2): 849-853.

Devasagayam, T.P.A., Pushpendran, C.K., Eapen, J. Differences in lipid peroxidation in rat liver rough and smooth microsomes. Biochimica et Biophysica Acta- Lipids and Lipid Metabolism. 1983; 750(1): 91-97.

Jayakar, V., Lokapur, V., Shantaram, M. In-vitro antioxidant and selective cytotoxicity of Garcinia cambogia and Garcinia indica leaf extracts on human kidney cancer cell line. International Journal of Research in Pharmaceutical Sciences. 2021 Jul 5;12(3):1718-1728.

Jayakar, V., Lokapur, V., Nityasree, B.R., Chalannavar, R.K., Lasrado, L.D., Shantaram, M. Optimization and green synthesis of zinc oxide nanoparticle using Garcinia cambogia leaf and evaluation of their antioxidant and anticancer property in kidney cancer (A498) cell lines. Biomedicine. 2021 Jul 7;41(2):206-222.

Bhattacharya, E., Pal, U., Dutta, R. Bhowmik, P., Biswas, S. Antioxidant, antimicrobial and DNA damage protecting potential of hot taste spices: a comparative approach to validate their utilization as functional foods. J Food Sci Technol. 2022; 59(3): 1173-1184.

Sathisha, A.D., Lingaraju, H.B., Sham Prasad. Evaluation of Antioxidant Activity of Medicinal Plant Extracts Produced for Commercial Purpose. E. Journal of Chemistry. 2011; 8(2):882-886.

Ali, M.A., Wahed, M.I., Khatune, N.A., Rahman, B.M., Barman, R.K., Islam, M.R. Antidiabetic and antioxidant activities of ethanolic extract of Semecarpus anacardium (Linn.) bark. BMC Complement Altern Med. 2015; 15:138.

Bibi, G., Ihsan-ul-Haq, Ullah, N., Mannan, A., Mirza, B. Antitumor, cytotoxic and antioxidant potential of Aster thomsonii extracts. Afr J Pharm Pharamacoli. 2011; 5(2): 252-258.




How to Cite

Das S, L. S. Hallur R, Baran Mandal A. Evaluation of Semecarpus kurzii Engler from Bay Islands for oxidative DNA damage protective activity and in vitro antioxidant potential. Biomedicine [Internet]. 2023 May 4 [cited 2024 Mar. 3];43(02):595-602. Available from: https://biomedicineonline.org/index.php/home/article/view/1961



Original Research Articles

Plum Analytics