Studies on antioxidant potential and total phenolic contents of dried powder and pulp of raw and ripe Carica papaya fruit
DOI:
https://doi.org/10.51248/.v43i02.2361Keywords:
Carica papaya, antioxidant, phenolic content, radical scavenging, bioactive compoundAbstract
Introduction and Aim: Antioxidants are frequently used as active ingredients in anti-aging supplements with the intention of preserving health and preventing diseases. However, recent research has concentrated on conventional therapies that have a long history of successfully treating several diseases. C. papaya is a good alternative and exhibits significant antioxidant, diuretic, antihyperglycemic, anticancer, analgesic, and depressive properties from a key plant constituent. In this study, total phenolic content, and antioxidant potential of raw and ripe Carica papaya were examined.
Materials and Methods: C. Samples of raw and ripe Carica papaya were collected from the local market of Dehradun. Dried powder and fresh pulp of the raw and ripe Carica papaya was individually subjected to phytochemical test principles. Total phenolic contents were estimated using the Folin-Ciocalteu and antioxidant potential using DPPH and ferric chloride reducing power assay method.
Results: The fresh pulp and powder of ripe Carica papaya showed high content of phenolics in fresh ripe pulp, fresh raw pulp, dried ripe powder and raw dried powder as 200, 195,185 7 mg/100 g respectively with an antioxidant activity DPPH radical scavenging activity, 80.2, and 75. .76.4 and 74.5 respectively which is comparable to butylated hydroxy anisole (BHA) and diphenhydramine (DPPH) radical scavenging activity.
Conclusion: The fresh pulp and powder of ripe Carica papaya showed wide antioxidant activity (DPPH) radical scavenging activity. Hence, it can be effectively used as an organic substitute for chemical compounds (BHA, BHT) which have a lot of harmful side effects. Moreover, phytochemical studies of Carica papaya have shown the presence of phenolic compounds well justified by estimation of content which are responsible for its potent antioxidant activity.
References
Shashni, B., Nagasaki, Y. Nitroxide radical-containing nanoparticles attenuate tumorigenic potential of triple negative breast cancer. Biomaterials. 2018; 178: 48-62.
Wu, C.C., Hung, C.N., Shin, Y.C., Wang, C.J, Huang, H.P. Myrciaria cauliflora extracts attenuate diabetic nephropathy involving the Ras signaling pathway in streptozotocin/nicotinamide mice on a high fat diet. Journal of food and drug analysis. 2016; 1; 24(1):136-146.
Pandey, V.P., Singh, S., Jaiswal, N., Awasthi, M., Pandey, B., Dwivedi, U.N., et al., Papaya fruit ripening: ROS metabolism, gene cloning, characterization, and molecular docking of peroxidase. Journal of Molecular Catalysis B: Enzymatic. 2013;30;98-105.
Kamemura, N. Butylated hydroxytoluene, a food additive, modulates membrane potential and increases the susceptibility of rat thymocytes to oxidative stress. Computational Toxicology. 2018; 1;6:32-38.
Kumar, N.S., Devi, P.S. The surprising health benefits of papaya seeds: A. J. Pharmacogn. Phytochem. 2017;6(1):424-429.
Anitha, M., Selvy, P.T, Palanisamy, V. Automated detection of white matter lesions in MRI brain images using spatio-fuzzy and spatio-possibilistic clustering models. Computer Science and Engineering. 2012 ; 1;2(2):1.
Sripanidkulchai, B., Wongpanich, V., Laupattarakasem, P., Suwansaksri, J., Jirakul Somchok, D. Diuretic effects of selected Thai indigenous medicinal plants in rats. Journal of Ethnopharmacology. 2001 ; 1;75(2-3):185-190.
Venkateshwarlu, E., Dileep, P., Sandhya, P. Evaluation of antidiabetic activity of Carica papaya seeds on streptozotocin-induced type-II diabetic rats. Journal of Advanced Scientific Research. 2013 ;10;4(02):38-41.
Akhtar, P., Yaakob, Z., Ahmed, Y., Shahinuzzaman, M., Hyder, M.K. Total phenolic contents and free radical scavenging activity of different parts of Jatropha species. Industrial Crops and Products. 2018 ; 1;30:365-370.
Lokapur V., Jayakar V., Shantaram M. Phytochemical investigation, chemical composition and in vitro antioxidant activities of various crude extracts of Holigarna ferrugenia Marchand. Medicinal Plants-International Journal of Phytomedicines and Related Industries. 2022; 14(1): 72-83.
Lokapur V., Jayakar V., Shantaram M. Preliminary phytochemical screening, physicochemical analysis and in-vitro antioxidant activity of selected Holigarna species-Endemic plant species of Western Ghats. Biomedicine. 2020;40(4):460-466.
Jayakar V., Lokapur V., Shantaram M. In-vitro antioxidant and selective cytotoxicity of Garcinia cambogia and Garcinia indica leaf extracts on human kidney cancer cell line. International Journal of Research in Pharmaceutical Sciences. 2021 Jul 5;12(3):1718-1728.
Lokapur V., Jayakar V., Divakar M.S., Chalannavar R.K., Lasrado L., Shantaram M. ZnO nanoparticles with spectroscopically controlled morphology, bioinspired from Holigarna grahamii (Wight) Kurz and delving its antioxidant and anticancer potential on A498 cell line. Materials Today Communications. 2022 Jun 1; 31: 103338.
Jayakar V., Lokapur V., Nityasree B.R., Chalannavar R.K., Lasrado L.D., Shantaram M. Optimization and green synthesis of zinc oxide nanoparticle using Garcinia cambogia leaf and evaluation of their antioxidant and anticancer property in kidney cancer (A498) cell lines. Biomedicine. 2021 Jul 7;41(2):206-222.
Jayakar V., Lokapur V., Shantaram M. Identification of the volatile bioactive compounds by GC-MS analysis from the leaf extracts of Garcinia cambogia and Garcinia indica. Medicinal Plants-International Journal of Phytomedicines and Related Industries. 2020;12(4):580-590.
Jose M., Varghese V. I., Jayakar V., Lokapur V., Srinivasa K., Shantaram M. Evaluation of antioxidant activities and free radical scavenging properties in mango leaves, husks of areca and coconut. Research Journal of Pharmaceutical Biological and Chemical Sciences. 2018 Jul 1;9(4):1607-1619.
Dadoosh, R.M. Evaluation of Phytochemical, Total phenolic and antioxidant activity of Carica papaya seed for its use in biosynthesis of gold nanoparticles. Egyptian Journal of Chemistry. 2021; 1;64(8):1-2.
Patil, R., Anwar, C., Govind, Vishwas, V., Bapat, R., Jadhav, J.S., et al., Optimization studies for extraction of antioxidants from Mucuna sanjappae seeds: A promising natural drug for oxidative stress management. Biomedicine. 2023; 43(1): 403-412.
Vinayaka, K.S., Raghavendra L.S., Prashith, K. Preliminary phytochemical analysis and in vitro antioxidant activity of Glochidion ellipticum Wight (Phyllanthaceae). Biomedicine. 2022; 42(1): 148-153.
Singleton, V.L., Orthofer, R., Lamuela-Raventós, R.M. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent in Methods in Enzymology. 1999; 299: 152-178.
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