Phytohormone synthesis for PDH activation and phytochemical screening for the treatment of endometriosis




Endometriosis, HPMC, pyruvate dehydrogenase, gibberellin, herbal therapy


Introduction and Aim: Endometriosis is a challenging gynecological disorder that causes pelvic pain and infertility attributed to the prevalence of ectopic endometrial tissue outside the uterine tract. Endometrial tissue can travel to other areas of the body for inexplicable reasons. There are, nevertheless, certain patterns. Endometriosis could be treated in several ways, based on characteristics such as age, fertility, symptom severity, and disease degree. All endometriosis medications are available as contraceptives, restricting their use to pregnant mothers or intending to get children. By comparing human peritoneal mesothelial cells (HPMCs) derived from the pelvic peritoneum of women with endometriosis to HPMCs from women without endometriosis, the researchers found that HPMCs from women with endometriosis had reduced glycolysis, mitochondrial respiration, and pyruvate dehydrogenase (PDH) and improved lactate production. There is no treatment for endometriosis, even though various medications and management alternatives are available. The present study was carried out to find novel medications to treat endometriosis from medicinal plants which appear to be a promising endeavor.


Methods and Materials: Phytohormone and phytochemical screening were performed to study the medication options that could possibly treat endometriosis. Phytochemicals were determined using GC-MS.


Results: The phytohormone activity and phytochemical components of Mentha piperita, Curcuma longa, and Andrographis paniculata were identified using GC MS and FT-IR analysis. This initial data from the analytical methods will make it easier to perform follow-up research on finding bioactive ingredients, determining their efficacy through in vivo tests, and proving their safety and efficacy through clinical trials to treat endometriosis.


Conclusion: Gibberellin, a phytohormone obtained from natural sources, is supposed to improve PDH activity and hence inhibit PDK. The demand for more modern medicines to increase survival in such cases remains unmet. As a result, there is an increasing interest in using herbal therapy to treat endometriosis.

Author Biographies

Shanmugapriya Rishikesan

Department of Bioengineering, Vels University (VISTAS), Old Pallavaram, Chennai, Tamil Nadu, India

BrindhaDevi Parthiban

Department of Bioengineering, Vels University (VISTAS), Old Pallavaram, Chennai, Tamil Nadu, India


Parasar, P., Ozcan, P., Terry, K.L. Endometriosis: Epidemiology, diagnosis and clinical management. Curr Obstet Gynecol Rep. 2017; 6(1):34-41.

Koninckx, P.R., Ussia, A., Adamyan, L., Wattiez, A., Gomel, V., Martin, D.C. Pathogenesis of endometriosis: the genetic/epigenetic theory. Fertil Steril. 2019;111(2):327-340.

Sudha, S.G., Mittelschmerz. Treatment of endometriosis. International Journal of Advances in Nursing Management. 2020;8(1):103.

Primolut, R.N., Repurposing dichloroacetate for the treatment of women with endometriosis. International Journal of Advances in Nursing Management, 2021;116(51):336-337.

Savant, P.B., Kareppa, M.S., Shinde, A.U. Effects of curcumin in management of endometriosis, a hormonal disorder in females. Research Journal of Pharmacognosy and Phytochemistry. 2021;13(4):182-186.

Bina, F., Soleymani, S., Toliat, T., Hajimahmoodi, M., Tabarrai, M., Abdollahi, M., et al., Plant-derived medicines for treatment of endometriosis: A comprehensive review of molecular mechanisms, Pharmacological Research, 2019;139:76-90

Horne, A.W., Ahmad, S.F., Carter, R., Simitsidellis, I., Greaves, E., Hogg, C. Repurposing dichloroacetate for the treatment of women with endometriosis. Proc Natl Acad Sci U S A. 2019; 116(51): 25389-25391.

Ilhan, M., Guragac Dereli, F.T., Akkol, E.K. Novel drug targets with traditional herbal medicines for overcoming endometriosis. Curr Drug Deliv. 2018;16(5):386-399.

Bina, F., Soleymani, S., Toliat, T., Hajimahmoodi, M., Tabarrai, M. Abdollahi, M. Plant derived medicines for treatment of endometriosis: A comprehensive review of molecular mechanisms. Pharmacological Research, 2019;139:76-90.

Balan, A., Moga, M.A., Dima, L., Dinu, C.G., Martinescu, C.C., Panait, D.E. An overview on the conservative management of endometriosis from a naturopathic perspective: Phytochemicals and Medicinal Plants, 2021;10:1-31.

Asohan, K., Krishnan, K.G., Kalaiselvan, A., Gokulakrishnan, K., Anand, T. Gas chromatography-mass spectrum analysis of bioactive components of the ethanol extract of Andrographis paniculata. Journal of Pharmaceutical and Biomedical Sciences 2012;20(15)

Mahanthesh, M.C., Gautam, G., Jalalpure, S.S. Development and screening of anticonvulsant polyherbal formulation. Res J Pharm Technol. 2017;10(5):1402-1416.

Mahadeva Rao, U.S., Shanmuga Sundaram, C., Sivakumar, J. Isolation and characterization of phytochemical constituents and its antibacterial activity of Brassica oleracea var acephala. Res J Pharm Technol. 2019; 12(1):297-302.

Kumar, R., Gautam, G.K., Pundir, S., Zaidi, S., Gupta, C. Treatment of human infertility. Asian Journal of Research in Pharmaceutical Sciences, 2021;11(2):160-164.

Wieser, F., Cohen, M., Gaeddert, A., Yu, J., Burks-Wicks, C., Berga, S.L. Evolution of medical treatment for endometriosis: Back to the roots?. Human Reproduction Update, 2007;13: 487-499.

Riemma, G., Lagana, A.S., Schiattarella, A., Garzon, S., Cobellis, L., Autiero, R. Ion channels in the pathogenesis of endometriosis: A cutting-edge point of view. International Journal of Molecular Sciences. 2020;21(3):1114

Missmer, S.A., Tu, F.F., Agarwal, S.K., Chapron, C., Soliman, A.M., Chiuve, S. Impact of endometriosis on life-course potential: A narrative review. International Journal of General Medicine. Dove Medical Press Ltd; 2021; 14: 9-25.

Brichant, G., Laraki, I., Henry, L., Munaut, C., Nisolle, M. New therapeutics in endometriosis: A review of hormonal, non-hormonal, and non-coding RNA treatments. Int J Mol Sci. 2021;22:10498

Fukui, A., Mai, C., Saeki, S., Yamamoto, M., Takeyama, R., Kato, T. Pelvic endometriosis and natural killer cell immunity. American Journal of Reproductive Immunology, 2021; 85(4): 13342

Lagana, A.S., Garzon, S., Gotte, M., Vigano, P., Franchi, M., Ghezzi, F., The pathogenesis of endometriosis: Molecular and cell biology insights. International Journal of Molecular Sciences, MDPI AG; 2019; 20(22):5615

Young, V.J., Brown, J.K., Saunders, P.T.K., Horne, A.W. The role of the peritoneum in the pathogenesis of endometriosis. Hum Reprod Update. 2013;19(5):558-569.

Endometriosis. Springer Tokyo. 2014; IX, 477: Edition 1

Sourial, S., Tempest, N., Hapangama, D.K. Theories on the pathogenesis of endometriosis. Int J Reprod Med. 2014; 2014:1-9.

Ahn, S. H., Edwards, A.K., Singh, S.S., Young, S.L., Lessey, B.A., Tayade, C. IL-17A Contributes to the pathogenesis of endometriosis by triggering proinflammatory cytokines and angiogenic growth factors. The Journal of Immunology, 2015; 195(6):2591-2600.

Gazvani, R., Templeton, A. New considerations for the pathogenesis of endometriosis. International Journal of Gynecology and Obstetrics. 2002;76(2):117-126.

Jayakar, V., Lokapur, V., Shantaram, M. In-vitro antioxidant and selective cytotoxicity of Garcinia cambogia and Garcinia indica leaf extracts on human kidney cancer cell line. International Journal of Research in Pharmaceutical Sciences. 2021 Jul 5;12(3):1718-1728.

Lokapur, V., Jayakar, V., Divakar, M. S., Chalannavar, R. K., Lasrado, L., Shantaram, M. ZnO nanoparticles with spectroscopically controlled morphology, bioinspired from Holigarna grahamii (Wight) Kurz and delving its antioxidant and anticancer potential on A498 cell line. Materials Today Communications. 2022 Jun 1;31:103338.

Lokapur, V., Jayakar, V., Shantaram, M. Preliminary phytochemical screening, physicochemical analysis and in-vitro antioxidant activity of selected Holigarna species-Endemic plant species of Western Ghats. Biomedicine. 2020;40(4):460-466.

Vasudha, M., Kalasad, M. N., Sharath, S. C., Gayathri, D. Fourier transform infrared spectroscopy analysis of Lactose hydrolysis by beta-galactosidase from Lactiplantibacillus plantarum GV54 and Lactiplantibacillus sp. GV66. Biomedicine. 2023 Feb 26;43(1):146-150.

Jayakar, V., Lokapur, V., Nityasree, B. R., Chalannavar, R. K., Lasrado, L. D., Shantaram, M. Optimization and green synthesis of zinc oxide nanoparticle using Garcinia cambogia leaf and evaluation of their antioxidant and anticancer property in kidney cancer (A498) cell lines. Biomedicine. 2021 Jul 7;41(2):206-222.

Lokapur, V., Jayakar, V., Shantaram, M. Phytochemical investigation, chemical composition and in vitro antioxidant activities of various crude extracts of Holigarna ferrugenia Marchand. Medicinal Plants-International Journal of Phytomedicines and Related Industries. 2022;14(1):72-83.

Jayakar, V., Lokapur, V., Shantaram, M. Identification of the volatile bioactive compounds by GC-MS analysis from the leaf extracts of Garcinia cambogia and Garcinia indica. Medicinal Plants-International Journal of Phytomedicines and Related Industries. 2020;12(4):580-590.




How to Cite

Rishikesan S, Parthiban B. Phytohormone synthesis for PDH activation and phytochemical screening for the treatment of endometriosis. Biomedicine [Internet]. 2023 Aug. 30 [cited 2023 Oct. 4];43(4):1123-9. Available from:



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