A study on placental expression of neuronal markers in intrauterine growth restriction

Upendhar Reddy Pulluru; Sudhakara Babu Chelli; Venkateshwar Reddy Muchinthala; Sthevaan V.; Sai Charitha; Sai Priya Reddy; Govindarajan Sumathy

doi:10.51248/.v43i5.2758

Authors

Upendhar Reddy Pulluru
Sudhakara Babu Chelli
Venkateshwar Reddy Muchinthala
Sthevaan V.
Sai Charitha
Sai Priya Reddy
Govindarajan Sumathy

DOI:

https://doi.org/10.51248/.v43i5.2758

Keywords:

IUGR, GFAP, NSE, immune expression, brain damage, neonates

Abstract

Introduction and Aim: The restriction of intrauterine growth (IUGR) has a 20% recurrence rate and is one of the leading causes of postnatal illness and death. The diagnosis of intrauterine retardation refers to the infant's increased risk of neurological issues over an extended period, neonatal morbidity, and mortality, and in utero death.

Materials and Methods: One hundred placenta samples were collected and divided into cases and controls. Clearance from the ethics committee was taken from the institute prior to the commencement of this study. Exclusion criteria include the patients with multiple pregnancies, unknown gestational age, gestational diabetes, and HIV. The inclusion criteria are the singleton pregnancy, normal and cesarean section, maternal age between 18-35 years and GA between 34 – 41 weeks. Standard immunohistochemistry protocols were followed for the study and glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE) markers were used as neuronal markers.

Results: Strong immunoreactivity of glial fibrillary acidic protein and neuron specific enolase was observed in fetal growth restriction placenta indicating perinatal brain damage of neonate.

Conclusion: In our study we observed strong positive immunoreactivity of GFAP and NSE in IUGR only. This study suggests that these markers are used to predict brain damage in IUGR neonates.

Author Biographies

Upendhar Reddy Pulluru

Department of Anatomy, SVS Medical College, Mahbubnagar, Telangana, India

Sudhakara Babu Chelli

Department of Anatomy, Government Medical College, Nizamabad, Telangana, India

Venkateshwar Reddy Muchinthala

Department of Anatomy, SVS Medical College, Mahbubnagar, Telangana, India

Sthevaan V.

Department of Pathology, Toxicology, Palamur Biosciences Private Limited, Mahabubnagar, Telangana, India

Sai Charitha

Department of Pathology, Toxicology, Palamur Biosciences Private Limited, Mahabubnagar, Telangana, India

Sai Priya Reddy

Department of Pathology, Toxicology, Palamur Biosciences Private Limited, Mahabubnagar, Telangana, India

Govindarajan Sumathy

Department of Anatomy, Sree Balaji Dental College & Hospital, Chennai, Tamil Nadu, India

References

Olshinka, K. R., Michaeli, J., Srebnik, N., Terletzky, S., Schreiber, L., Farkash, R., et al., Recurrent intrauterine growth restriction: Characteristic placental histopathological features and association with prenatal vascular Doppler. Archives of Gynecology and Obstetrics. 2019; 300(6):1583-1589.

Kouvalainen, K., Pynnonen, A.l., Makarainen, M., Peltonen, T. Weights of placental membranes and umbilical cord. Duodecim. 1971; 87:1210-1214.

Fox, H. Pathology of the placenta. 2nd ed. London (UK): WB Saunders, 1997.

Lewis, S.H., Perrin, E. Pathology of the placenta, 2nd ed. New York: Churchill Livingstone, 1999.

Mazarico, E., Llurba, E., Cumplido, R., Valls, A., Melchor, J.C., Iglesias, M., et.al., Neural injury markers in intrauterine growth restriction and their relation to perinatal outcomes. Pediatric Research. 2017; 82(3):452-457.

Giuseppe, D., Sergio, C., Pasqua, B., Giovanni, L.V., Salvatore, C., Frigiola, A., et al., Perinatal asphyxia in preterm neonates leads to serum changes in protein S-100 and neuron specific enolase. Curr Neurovasc Res. 2009; 6 (2):110-116.

Elimian, A., Figueroa, R., Verma, U., Visintainer, P., Sehgal, P.B., Tejani, N., et.al., Amniotic fluid neuron-specific enolase: a role in predicting neonatal neurologic injury. Obstet Gynecol. 1998; 92:546-550.

Wijnberger, L.D.E. Nikkels, P.G.J., van Dongen, A.J.C.M., Noorlander, C.W., Mulder, E.J.H., Schrama, L.H., et al., Expression in the placenta of neural markers for perinatal brain damage. Pediatr Res. 2002;51(4):492-496.

Velipasaoglu, M., Yurdakok, M., Ozyuncu, O., Portakal, O., Deren, O. Neural injury markers to predict neonatal complications in intrauterine growth restriction. J Obstet Gynecol. 2015;35:555-560.

Morozova, A.Y., Milyutina, Y.P., Kovalevskaya O.V. K., Arutjunyan, A.V., Evsyukova, I.I. Neuron-specific enolase and brain-derived neurotrophic factor levels in umbilical cord blood in full-term newborns with intrauterine growth retardation. Journal of obstetrics and women's diseases. 2019; 68(1):29-36.

Eng, L.F. Ghirnikar, R.S., Lee, Y.L. Glial fibrillary acidic protein: GFAP-thirty-one years (1969–2000). Neurochemical Research. 2000; 25 (9-10); 1439-1451.

Kelleher, M.A., Palliser, H.K., Walker, D.W., Hirst, J.J. Sex-dependent effect of a low neurosteroid environment and intrauterine growth restriction on foetal guinea pig brain development. Journal of Endocrinology. 2011; 208(3):301- 309.

Liu, J., Liu, L., Chen, H. Antenatal taurine supplementation for improving brain ultrastructure in fetal rats with intrauterine growth restriction. Neuroscience. 2011; 181:265-270.

Djebaili, M., Guo, Q., Pettus, E.H., Hoffman, S.H., Stein, D.G. The neurosteroids progesterone and allopregnanolone reduce cell death, gliosis, and functional deficits after traumatic brain injury in rats. Journal of Neurotrauma 2005; 22 (1); 106-118.