Harnessing the power of plants: How nano-formulated plant-based compounds are revolutionizing pathogen treatment?


  • Suganya Kannan
  • Jeyakumar Balakrishnan
  • Mohan Sundaram




Plant-based compounds, nano vehicles, pathogens, cell membranes, enzyme activity, quorum sensing, oxidative stress


Antibiotic resistance is a global public health crisis that has led to an urgent need for new strategies to combat bacterial infections. One promising approach is the use of natural compounds derived from plants, which have shown potential in combating pathogens. However, the efficacy of plant-based compounds can be limited by their bioavailability and the difficulty of targeting specific pathogens. The development of nano vehicles that encapsulate plant-based compounds has provided a solution to these challenges. In this article, we provide an overview of the various mechanisms by which nano-formulated plant-based compounds combat pathogens, including disruption of cell membranes, inhibition of enzyme activity, interference with quorum sensing, oxidative stress, immunomodulation, and interference with biofilm formation. We also present case studies demonstrating the efficacy of nano-formulated plant-based compounds in treating various bacterial and fungal infections, including candidiasis, tuberculosis, periodontitis, acne, and urinary tract infections. Finally, we discuss promising areas for future research and development of nano-formulated plant-based compounds, including combination therapy, synergistic effects, personalized medicine, alternative to traditional antibiotics, environmental applications, novel delivery systems, and large-scale production.

Author Biographies

Suganya Kannan

Central Research Laboratory, Vinayaka Mission’s Medical College and Hospital, Vinayaka Mission’s Research Foundation (Deemed to be University), Karaikal, Puducherry, India

Jeyakumar Balakrishnan

Central Research Laboratory, Vinayaka Mission’s Medical College and Hospital, Vinayaka Mission’s Research Foundation (Deemed to be University), Karaikal, Puducherry, India

Mohan Sundaram

Department of Microbiology, Vinayaka Mission’s Medical College and Hospital, Vinayaka Mission’s Research Foundation (Deemed to be University), Karaikal, Puducherry, India


Centers for Disease Control and Prevention. Antibiotic resistance threats in the United States, 2019. Atlanta, GA: US Department of Health and Human Services, CDC; 2019.

World Health Organization. Antimicrobial resistance. Geneva, Switzerland: WHO; 2020.

World Health Organization. Ten threats to global health in 2019. Geneva, Switzerland: WHO; 2019.

Frieri, M., Kumar, K., Boutin, A. Antibiotic resistance. Journal of infection and public health, 2017;10(4), 369-378.

Cowan, M.M. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12(4):564-582.

Lokapur, V., Jayakar, V., Shantaram, M. Preliminary phytochemical screening, physicochemical analysis and in-vitro antioxidant activity of selected Holigarna species-Endemic plant species of Western Ghats. Biomedicine. 2020;40(4):460-466.

Hu, C.M.J., Aryal, S, Zhang, L. Nanoparticle-assisted combination therapies for effective cancer treatment. Ther Deliv. 2010;1(2):323-334.

Jaiswal, M., Dudhe, R., Sharma, P.K. Nanoemulsion: an advanced mode of drug delivery system. 3 Biotech. 2015;5(2):123-127.

Pratap, G. K., Shantaram, M. Green synthesis of silver nanoparticles (Ag-NPs) from Olea dioica Roxb., leaf extracts and its biological activity. Biomedicine. 2019;39(4):544-549.

Singh, R., Lillard, Jr J.W. Nanoparticle-based targeted drug delivery. Exp Mol Pathol. 2009;86(3):215-223.

Jain, K., Kesharwani, P, Gupta U, Jain, N. K. Dendrimer toxicity: let's meet the challenge. Int J Pharm. 2010;394(1-2):122-142.

Upaganlawar, A., Polshettiwar, S., Raut, S., Tagalpallewar, A., Pande, V. Effective Cancer Management: Inimitable Role of Phytochemical Based Nano- Formulations. Curr Drug Metab. 2022;23(11):869-881.

Guerra, F., Trevino, J., Zavala, G. Quercetin as a potential booster for antibiotics against drug-resistant bacteria. Antibiotics. 2020;9(7):400.

Pathak, N., Singh, P., Singh, P. K., Sharma, S., Singh, R. P., Gupta, A., et al., Biopolymeric nanoparticles based effective delivery of bioactive compounds toward the sustainable development of anticancerous therapeutics. Frontiers in Nutrition, 2022; 9, 963413.

Arokiyaraj, S., Saravanan, M., Prakash, N. U., Arasu, M. V., Vijayakumar, B.,Vincent, S. Enhanced antibacterial activity of iron oxide magnetic nanoparticles treated with Argemone mexicana L. leaf extract: an in vitro study. Materials Research Bulletin, 2013; 48(9), 3323-3327.

Laxminarayan, R., Matsoso, P., Pant, S., Brower, C., Røttingen, J. A., Klugman, K., et al., Access to effective antimicrobials: a worldwide challenge. The Lancet, 2016;387(10014), 168-175.

Graham, J.P., Eisenberg, J.N.S., Trueba, G., Zhang, L, Johnson TJ. Small-scale food animal production and antimicrobial resistance: mountain, molehill, or something in-between? Environ Health Perspect. 2019;127(10):104002.

World Health Organization. Global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. Geneva, Switzerland: WHO; 2017.

Spellberg, B., Bartlett, J.G., Gilbert, D.N. The future of antibiotics and resistance. N Engl J Med. 2013;368(4):299-302.

Centers for Disease Control and Prevention. Antibiotic resistance threats in the United States, 2019. Atlanta, GA: US Department of Health and Human Services, CDC; 2019.

World Health Organization. Global action plan on antimicrobial resistance. 2015. Available from: https://www.who.int/antimicrobial-resistance/global-action-plan/en/.

Laxminarayan, R., Chaudhury, R. R. Antibiotic resistance in India: drivers and opportunities for action. PLoS Medicine. 2016; 13(3): e1001974.

Global Antibiotic Resistance Partnership (GARP)-India Working Group. Rationalizing antibiotic use to limit antibiotic resistance in India. The Indian Journal of Medical Research, 2011;134(3): 281.

Zou, Q., Li, Y., Zhang, L., Zuo, Y., Li, J., Li, J. Antibiotic delivery system using nano hydroxyapatite/chitosan bone cement consisting of berberine. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials. 2009;89(4):1108-1117.

Campini, P. A. L., de Oliveira, E. R., Camani, P. H., da Silva, C. G., Yudice, E. D. C., de Oliveira, S. A., et al., Assessing the efficiency of essential oil and active compounds/poly (lactic acid) microcapsules against common foodborne pathogens. International Journal of Biological Macromolecules. 2021;186: 702-713.

Miranda Cadena, K., Dias, M., Costa Barbosa, A., Collins, T., Marcos Arias, C., Eraso, E et al., Development and characterization of monoolein-based liposomes of carvacrol, cinnamaldehyde, citral, or thymol with anti-candida activities. Antimicrobial agents and chemotherapy. 2021;65(4): 10-1128.

Nazli, A., He, D. L., Liao, D., Khan, M. Z. I., Huang, C., He, Y. Strategies and progresses for enhancing targeted antibiotic delivery. Advanced Drug Delivery Reviews. 2022;114502.

Otoni, C. G., de Moura, M. R., Aouada, F. A., Camilloto, G. P., Cruz, R. S., Lorevice, et al., Antimicrobial and physical-mechanical properties of pectin/papaya puree/cinnamaldehyde nanoemulsion edible composite films. Food Hydrocolloids. 2014;41:188-194.

Kumar, A., Singh, P. P., Kumar, M., Prakash, B. Nanoencapsulated plant-based antifungal formulation against the Aspergillus flavus and aflatoxin B1 contamination: Unraveling the biochemical and molecular mechanism of action. International Journal of Food Microbiology. 2022;372: 109681.

Agel, M. R., Baghdan, E., Pinnapireddy, S. R., Lehmann, J., Schaefer, J., Bakowsky, U. Curcumin loaded nanoparticles as efficient photoactive formulations against gram positive and gram negative bacteria. Colloids and Surfaces B: Biointerfaces, 2019;178: 460-468.

Nallamuthu, I., Parthasarathi, A., Khanum, F. Thymoquinone loaded PLGA nanoparticles: antioxidant and anti microbial properties. International Current Pharmaceutical Journal. 2013;2(12): 202-207.

Moraes-Lovison, M., Marostegan, L. F., Peres, M. S., Menezes, I. F., Ghiraldi, M., Rodrigues, R. A., et al., Nanoemulsions encapsulating oregano essential oil: Production, stability, antibacterial activity and incorporation in chicken pate. Lwt. 2017;77: 233-240.

Haba, E., Bouhdid, S., Torrego-Solana, N., Marques, A. M., Espuny, M. J., Garcia-Celma, M. J., et al., Rhamnolipids as emulsifying agents for essential oil formulations: antimicrobial effect against Candida albicans and methicillin resistant Staphylococcus aureus. International journal of pharmaceutics, 2014;476(1-2): 134-141.

Ngan, T. T. K., Hien, T. T., Tien, L. X., Toan, T. Q. Chemical compositions and stability of Vietnamese Homalomena occulta essential oil under the influence of storage conditions. Egyptian Journal of Chemistry. 2022; 65(7): 23-31.

Najafi Taher, R., Ghaemi, B., Kharazi, S., Rasoulikoohi, S., Amani, A. Promising antibacterial effects of silver nanoparticle loaded tea tree oil nanoemulsion: A synergistic combination against resistance threat. Aaps Pharm Sci Tech. 2018;19: 1133-1140.

Swain, S. S., Paidesetty, S. K., Padhy, R. N., Hussain, T. Nano-technology platforms to increase the antibacterial drug suitability of essential oils: A drug prospective assessment. OpenNano, 2022;100115.

Adhavan, P., Kaur, G., Princy, A., Murugan, R. Essential oil nanoemulsions of wild patchouli attenuate multi drug resistant gram positive, gram negative and Candida albicans. Industrial Crops and Products. 2017;100: 106-116.

Sotelo Boyas, M., Correa Pacheco, Z., Bautista-Banos, S., Gomez, Y. G. Release study and inhibitory activity of thyme essential oil loaded chitosan nanoparticles and nanocapsules against foodborne bacteria. International Journal of Biological Macromolecules. 2017;103: 409-414.

Tseng, T. L., Chen, M. F., Liu, C. H., Hsu, Y. H., Lee, T. J. Oroxylin A suppresses adhesion molecules expression and endothelial barrier disruption in endotoxemic arteries. The FASEB Journal. 2017; 31: 827-813.

Zhang, Z., Li, X., Sang, S., McClements, D. J., Chen, L., Long, J. Polyphenols as plant-based nutraceuticals: health effects, encapsulation, nano-delivery, and application. Foods. 2022;11(15): 2189.

Adeyemi, S.B., Akere, A.M., Orege, J. I., Ejeromeghene, O, Orege, O.B., Akolade, J.O. Polymeric nanoparticles for enhanced delivery and improved bioactivity of essential oils. Heliyon. 2023; e16543

Huang, Y., Deng, S., Luo, X., Liu, Y., Xu, W., Pan, J., et al., Evaluation of intestinal absorption mechanism and pharmacokinetics of curcumin-loaded galactosylated albumin nanoparticles. International Journal of Nanomedicine. 2019; 9721-9730.

Lee, Y., Lee, J., Chung, C. In vitro and in vivo anti-periodontitis effects of catechin-loaded polymeric nanoparticles. J Periodontal Res. 2018;53(5):697-708.

Sintov, A., Shapiro, B., Bashankaev, B. Nano-formulated tea tree oil attenuates bacterial growth and acne formation. J Invest Dermatol. 2018;138(5):S14.

Zorofchian Moghadamtousi, S., Abdul Kadir, H., Hassandarvish, P., Tajik, H., Abubakar, S., Zandi, K. A review on antibacterial, antiviral, and antifungal activity of curcumin. BioMed research international, 2014;186864.

Jardim, K. V., Siqueira, J. L. N., Bao, S. N., Parize, A. L. In vitro cytotoxic and antioxidant evaluation of quercetin loaded in ionic cross-linked chitosan nanoparticles. Journal of Drug Delivery Science and Technology, 2022;74: 103561.




How to Cite

Kannan S, Balakrishnan J, Sundaram M. Harnessing the power of plants: How nano-formulated plant-based compounds are revolutionizing pathogen treatment?. Biomedicine [Internet]. 2023 Aug. 30 [cited 2023 Oct. 4];43(4):1094-101. Available from: https://biomedicineonline.org/index.php/home/article/view/2768

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