Phytochemical analysis and antibacterial activity of traditional plants for the inhibition of DNA gyrase
Keywords:Topoisomrases, Staphylococcus aureus, Solanum nigrum, Vitex negundo, Euphorbia hirta, alkaloids, terpenoids
Introduction and Aim: DNA gyrase is a class of Type II Topoisomerases that plays an important role in bacterial viability. It is found in all bacteria and is involved in replication, repair, recombination, and DNA transcription. Negative supercoiling of bacterial DNA by DNA gyr B is essential in replication which further influences all the metabolic activities. Staphylococcus aureus (ATCC 25923) is one of the pathogens that can modify its genome easily under multidrug resistance. In this study, the activity of medicinal compounds to inhibit DNA gyrB is explored. Plant species Solanum nigrum, Vitex negundo, and Euphorbia hirta were studied for the potential plant-based molecules. The compounds alkaloids, glycosides, flavonoids, and terpenoids were considered to have high-potential targets. The study focuses on DNA gyrase as a target and shows insights into future drug development. The research focuses on the discovery of novel plant-based therapeutic compounds to target DNA gyrase B activity.
Methods and Materials: Phytochemical screening was performed to study the medication options that could inhibit DNA gyrB. Phytochemicals were determined using GC-MS.
Results: Utilizing GC MS and FT-IR analysis, the phytochemical constituents of Solanum nigrum, Vitex negundo, and Euphorbia hirta were discovered. It will be simpler to do a follow-up study on discovering bioactive compounds and evaluating their effectiveness in inhibiting DNA gyrB with the help of this preliminary data from the analytical procedures.
Conclusion: There are countless applications for the phytochemicals that medicinal plants produce. Staphylococcus aureus will be stopped by DNA gyrB inhibition. The study employs DNA gyrase as its target and provides information on potential therapeutic targets. The goal of the study is to identify innovative plant-based medicinal molecules that specifically target DNA gyrase B activity.
Mahal SN, Turki AM, Abdulkareem EH. Effects of silver nanoparticles on multiple drug-resistant strains of Staphylococcus aureus from periodontal infection: An alternative approach for antimicrobial therapy. Biomedicine. 2023 Jul 1;43(3):908-914.
Tse, Y.C., Wang, J.C. E. coli and M. luteus DNA topoisomerase I can catalyze catenation or decatenation of double-stranded DNA rings. Cell. 1980;22(1):269-276.
Wang, J.C. Cellular roles of DNA topoisomerases: a molecular perspective. Nature Reviews Molecular Cell Biology. 2002;3(6):430-440.
Singh, S.B. Discovery and development of kibdelomycin, a new class of broad-spectrum antibiotics targeting the clinically proven bacterial type II topoisomerase. Bioorganic & Medicinal Chemistry. 2016; 24(24):6291-6297.
Brown, P.O., Cozzarelli, N.R. A sign inversion mechanism for enzymatic supercoiling of DNA. Science. 1979; 206(4422):1081-1083.
Klahn, P., Brönstrup, M. New structural templates for clinically validated and novel targets in antimicrobial drug research and development. How to overcome the antibiotic crisis: Facts, challenges, technologies and future perspectives. 2016: 398:365-417.
Cozzarelli, N.R. DNA gyrase and the supercoiling of DNA. Science. 1980;207(4434):953-960.
Gellert, M., Mizuuchi, K., O'Dea, M.H., Nash, H.A. DNA gyrase: an enzyme that introduces superhelical turns into DNA. Proceedings of the National Academy of Sciences.1976;73(11):3872-3876.
Foster, T.J. Staphylococcus aureus. Molecular Medical Microbiology. 2002;2: 839-888.
Hussein AR, Khalaf ZZ. Antibacterial activity of klebocin against methicillin resistance Staphylococcus aureus. Biomedicine. 2022 Nov 14;42(5):983-987.
Taylor, T.A., Unakal, C.G. Staphylococcus aureus; Stat Pearls. Publishing: Treasure Island, FL, USA. 2017.
Chambers, H.F. The changing epidemiology of Staphylococcus aureus. Emerging infectious diseases. 2001;7(2):178.
Otto, M. Staphylococcus aureus toxins. Current Opinion in Microbiology. 2014;17:32-37.
Chambers, H.F., DeLeo, F.R. Waves of resistance: Staphylococcus aureus in the antibiotic era. Nature Reviews Microbiology. 2009;7(9):629-641.
Agidew, M.G. Phytochemical analysis of some selected traditional medicinal plants in Ethiopia. Bulletin of the National Research Centre. 2022;46(1):1-22.
Lokapur, V., Jayakar, V., Shantaram, M. Preliminary phytochemical screening, physicochemical analysis and in-vitro antioxidant activity of selected Holigarna species-Endemic plant species of Western Ghats. Biomedicine. 2020;40(4):460-466.
Hemasudha, T.S., Yuvarani, S., Rajakumari, K. Combination of Azadirachta indica and Phyllanthus acidus-A complete evaluation and characterization study. Research Journal of Pharmacy and Technology. 2019;12(6):3048-3052.
Jayakar, V., Lokapur, V., Nityasree, B.R., Chalannavar, R.K., Lasrado, L.D., Shantaram, M. Optimization and green synthesis of zinc oxide nanoparticle using Garcinia cambogia leaf and evaluation of their antioxidant and anticancer property in kidney cancer (A498) cell lines. Biomedicine. 2021 Jul 7;41(2):206-222.
Lokapur, V., Jayakar, V., Divakar, M.S., Chalannavar, R.K., Lasrado, L., Shantaram, M. ZnO nanoparticles with spectroscopically controlled morphology, bioinspired from Holigarna grahamii (Wight) Kurz and delving its antioxidant and anticancer potential on A498 cell line. Materials Today Communications. 2022 Jun 1;31:103338.
Olivia, N.U., Goodness, U.C., Obinna, O.M. Phytochemical profiling and GC-MS analysis of aqueous methanol fraction of Hibiscus asper leaves. Future Journal of Pharmaceutical Sciences. 2021;7:1-5.
Lokapur, V., Jayakar, V., Shantaram, M. Phytochemical investigation, chemical composition and in vitro antioxidant activities of various crude extracts of Holigarna ferrugenia Marchand. Medicinal Plants-International Journal of Phytomedicines and Related Industries. 2022;14(1):72-83.
Jayakar, V., Lokapur, V., Shantaram, M. Identification of the volatile bioactive compounds by GC-MS analysis from the leaf extracts of Garcinia cambogia and Garcinia indica. Medicinal Plants-International Journal of Phytomedicines and Related Industries. 2020;12(4):580-590.
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