Dose dependent effect of silver nitrate on enhanced production of gymnemic acid in cell suspension cultures of Gymnemasylvestre R. Br

Authors

  • Jarubandi Rajashekar
  • Vadlapudi Kumar
  • DittekoppaVijendra Poornima
  • Gajula Hari
  • Torankumar Sannabommaji
  • D Raghuramulu

DOI:

https://doi.org/10.51248/.v41i1.527

Keywords:

Gymnemic acid, HPLC, elicitation

Abstract

 

Introduction and Aim: Gymnemasylvestre is rich source of triterpenoid saponins and is popularly known for its anti-diabetic potential. Gymnemic acid is chiefly responsible for its therapeutic potential. In fact, gymnemic acid is a group of compounds known as gymnemagenin (oleanane group) and other related compounds are gymnemasides (dammaranes). Despite of its immense medicinal importance, very few studies were conducted for enhanced gymnemic acid production. The present study undertaken for the enhanced gymnemic acid production using silver nitrate (AgNO3) as effective elicitor and its influence on other culture parameters.  

Materials and Methods:Callus culture was initiated for cell suspension culture establishment and is used to study the elicitor effect. Different doses of AgNO3 used at varied culture periods and its mediated effect on growth index, pH, EC and MDA content was conducted. Gymnemic acid production was estimated by HPLC.

 

Results:Cell growth was positively influenced by AgNO3 with concomitant change in pH and EC of the medium. Apparent rise in MDA content in response to increased AgNO3 dosage was observed and is positively correlated with enhanced gymnemic acid production. Dose dependent effect of AgNO3 was observed at different culture periods used under study. 

Conclusion: Substantial improvements of gymnemic acid produced in response to AgNO3 at 100µM dosage can be used for its enhanced production at industrial scale. It also offers opportunities for studying elicitor influence on other parameters for gymnemic acid production

Author Biographies

Jarubandi Rajashekar

Department of Biochemistry, Davangere University, Shivagangothri, Davangere-577 007 Karnataka India

Vadlapudi Kumar

Department of Biochemistry, Davangere University, Shivagangothri, Davangere-577 007 Karnataka India

DittekoppaVijendra Poornima

Department of Biochemistry, Davangere University, Shivagangothri, Davangere-577 007 Karnataka India

Gajula Hari

Department of Biochemistry, Davangere University, Shivagangothri, Davangere-577 007 Karnataka India

Torankumar Sannabommaji

Department of Biochemistry, Davangere University, Shivagangothri, Davangere-577 007 Karnataka India

D Raghuramulu

Department of Botany, Government Degree College for Men, Kurnool-518 001, Andhra Pradesh India

References

Sarker, P., Rahman, M.M., Khan, F., Ming, L.C., Mohamed, I.N., Zhao, C., Rashid, M.A. Comprehensive review on phytochemicals, pharmacological and clinical potentials of Gymnemasylvestre. Front.Pharmacol. 2019;10:1223.

Tiwari, P., Ahmad, K., Hassan Baig, M. Gymnemasylvestre for diabetes: from traditional herb to future’s therapeutic. Cur.Pharm.Design. 2017;23(11):1667-1676.

Ye, W.C., Zhang, Q.W., Liu, X., Che, C.T., Zhao, S.X. Oleananesaponins from Gymnemasylvestre. Phytochem. 2000;53(8):893-899.

Yang, L., Wen, K.S., Ruan, X., Zhao, Y.X., Wei, F., Wang, Q. Response of plant secondary metabolites to environmental factors. Molecules. 2018;23(4):762.

Namdeo, A.G. Plant cell elicitation for production of secondary metabolites: a review. Pharmacog. Rev. 2007; 1(1):69-79.

Isah, T. Stress and defence responses in plant secondary metabolites production. Biol. Res. 2019;52(1):39.

Ramirez-Estrada, K., Vidal-Limon, H., Hidalgo, D., Moyano, E., Golenioswki, M., Cusidó, R.M. et al.,Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules. 2016; 21(2):182.

Vijendra, P.D., Jayanna, S.G., Kumar, V., Sannabommaji, T., Rajashekar, J., Gajula, H. Product enhancement of triterpenoid saponins in cell suspension cultures of LeucasasperaSpreng. Ind. Crop. Prod. 2020;156:112857.

Veerashree, V., Anuradha, C.M., Kumar, V. Elicitor-enhanced production of gymnemic acid in cell suspension cultures of Gymnemasylvestre R. Br. Plant Cell. Tiss. Organ. Cult. 2012;108(1):27-35.

Chodisetti, B., Rao, K., Gandi, S., Giri, A. Gymnemic acid enhancement in the suspension cultures of Gymnemasylvestre by using the signaling molecules—methyl jasmonate and salicylic acid. In Vitro Cell Dev.Biol-Plant. 2015;51(1):88-92.

Chung, I.M., Rajakumar, G., Subramanian, U., Venkidasamy, B., Thiruvengadam, M. Impact of copper oxide nanoparticles on enhancement of bioactive compounds using cell suspension cultures of Gymnemasylvestre (Retz.) R. Br. Appl. Sci. Basel. 2019;9(10):2165.

Murashige, T., Skoog, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol.Plant. 1962;15(3):473-497.

Heath, R.L., Packer, L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 1968;125(1):189-198.

Xing, B., Yang, D., Guo, W., Liang, Z., Yan, X., Zhu, Y. et al., Ag+ as a more effective elicitor for production of tanshinones than phenolic acids in Salvia miltiorrhiza hairy roots. Molecules. 2015; 20(1):309-324.

Hari, G., Vadlapudi, K., Vijendra, P.D., Rajashekar, J., Sannabommaji, T., Basappa, G. A combination of elicitor and precursor enhances psoralen production in Psoraleacorylifolia Linn. Suspension Cultures. Ind. Crops Prod. 2018; 15(124):685-691.

Cai, Z., Kastell, A., Speiser, C., Smetanska, I. Enhanced resveratrol production in Vitisvinifera cell suspension cultures by heavy metals without loss of cell viability. ApplBiochemBiotechnol. 2013; 171:330-340.

Pan, Z.W., Wang, H.Q., Zhong, J.J. Scale-up study on suspension cultures of Taxuschinensis cells for production of taxanediterpene. Enzyme.Microb. Technol. 2000; 15,27(9):714-723.

Mahendran, D., Kavikishore, P.B., Sreeramanan, S., Venkatachalam P. Enhanced biosynthesis of colchicine and thiocolchicoside contents in cell suspension cultures of Gloriosasuperba L. exposed to ethylene inhibitor and elicitors. Ind. Crop Prod. 2018; 15,120:123-130.

Sathish, S., Vasudevan, V., Karthik, S., Pavan, G., Manickavasagam, M. Enhanced l-Dopa production from elicited cell suspension culture of Hybanthusenneaspermus (L.) F. Muell. Plant Biotechnol. Rep. 2019;3(6):613-621.

Rahimi A., Hasanloo, T.,Bihamta, M. R. Enhanced production of silymarin by Ag+ elicitor in cell suspension cultures of Silybummarianum. Pharm. Biol. 2010; 48: 708-715.

Zhao, J., Davis, L.C., Verpoorte, R. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol.Adv. 2005; 23(4):283-333.

You, J., Chan Z. ROS regulation during abiotic stress responses in crop plants. Front.PlantSci. 2015; 8,6:1092.

Ali, M., Baek, K.H. Jasmonic acid signalling pathway in response to abiotic stresses in plants. Int. J. Mol. Sci. 2020. 21(2):621.

Narayani, M., Srivastava, S. Elicitation: a stimulation of stress in in vitro plant cell/tissue cultures for enhancement of secondary metabolite production. Phytochem. Rev. 2017; 16(6):1227-1252.

Downloads

Published

2021-04-03

How to Cite

1.
Rajashekar J, Kumar V, Poornima D, Hari G, Sannabommaji T, Raghuramulu D. Dose dependent effect of silver nitrate on enhanced production of gymnemic acid in cell suspension cultures of Gymnemasylvestre R. Br. Biomedicine [Internet]. 2021Apr.3 [cited 2021Apr.14];41(1):16-22. Available from: https://biomedicineonline.org/index.php/home/article/view/527

Issue

Section

Original Research Articles