Identification of novel potent pancreatic lipase inhibitors from Ficus racemosa

Authors

  • A. Vijaya Anand
  • V. Bharathi
  • G. Bupesh
  • Jaya Lakshmi
  • K. Meenakshi Sundaram
  • . M. Saradhadevi

DOI:

https://doi.org/10.51248/.v41i1.528

Keywords:

Obesity, pancreatic lipase enzymes, phytochemical, Protein Data Bank

Abstract

Introduction and Aim: Obesity is a disorder of lipid metabolism and continues to be a global problem, ranking fifth for deaths worldwide. It is considered to be main reason for a number of physiological changes that resulting in many metabolic disorders such as cardiovascular diseases, diabetes, musculoskeletal disorders and various cancer types. Obesity develops from long-termed physiological imbalances in energy expenditure.. One of the therapeutic strategies in managing obesity is inhibition of pancreatic lipase, a key enzyme responsible for the digestion of fats and triglycerides.  The aim of the present study was to synthesize silver nanoparticles (Ag-NP) from Ficus racemosa fruit extract and to analyze its activity on pancreatic lipase enzyme using spectroscopic, in vitro and in silico methods.

Materials and Methods: The phytoconstituent were separated in Ficus racemosa fruit extract by thin layer chromatography technique. The bioactive compound was also identified by gas chromatography-mass spectrometry analysis. Pancreatic lipase inhibition assay was performed in vitro and confirmed by molecular docking analysis. 

Results: The F. racemosa fruit showed the highest pancreatic lipase inhibitory activity. Molecular docking studies also exhibited good binding affinity of compounds (Diethyl Phthalate) with pancreatic lipase enzymes. 

Conclusion: Finally, it is concluded that further derivation of such compounds could serve as the new templates for obesity.

Author Biographies

A. Vijaya Anand

Department of Human Genetics and Molecular Biology, 5Department of Biochemistry, Bharathiar University, Coimbatore, Tamil Nadu, India

V. Bharathi

Biological and Bioinformatics Research Centre, Trichy, Tamil Nadu, India

G. Bupesh

Department of Forest Science, Nagaland University, Lumami-798627, India

Jaya Lakshmi

Department of Biochemistry, Shrimati Indira Gandhi College, Trichy, Tamil Nadu, India

K. Meenakshi Sundaram

Department of Forest Science, Nagaland University, Lumami-798627, India

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Published

2021-04-03

How to Cite

1.
Vijaya Anand A, Bharathi V, Bupesh G, Lakshmi J, Meenakshi Sundaram K, Saradhadevi . M. Identification of novel potent pancreatic lipase inhibitors from Ficus racemosa. Biomedicine [Internet]. 2021Apr.3 [cited 2021Apr.14];41(1):23-30. Available from: https://biomedicineonline.org/index.php/home/article/view/528

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Original Research Articles