Identification of novel potent pancreatic lipase inhibitors from Ficus racemosa

Authors

  • A. Vijaya Anand
  • V. Bharathi
  • G. Bupesh
  • Jaya Lakshmi
  • K. Meenakshi Sundaram
  • . M. Saradhadevi

DOI:

https://doi.org/10.51248/.v41i1.528

Keywords:

Obesity, pancreatic lipase enzymes, phytochemical, Protein Data Bank

Abstract

Introduction and Aim: Obesity is a disorder of lipid metabolism and continues to be a global problem, ranking fifth for deaths worldwide. It is considered to be main reason for a number of physiological changes that resulting in many metabolic disorders such as cardiovascular diseases, diabetes, musculoskeletal disorders and various cancer types. Obesity develops from long-termed physiological imbalances in energy expenditure.. One of the therapeutic strategies in managing obesity is inhibition of pancreatic lipase, a key enzyme responsible for the digestion of fats and triglycerides.  The aim of the present study was to synthesize silver nanoparticles (Ag-NP) from Ficus racemosa fruit extract and to analyze its activity on pancreatic lipase enzyme using spectroscopic, in vitro and in silico methods.

Materials and Methods: The phytoconstituent were separated in Ficus racemosa fruit extract by thin layer chromatography technique. The bioactive compound was also identified by gas chromatography-mass spectrometry analysis. Pancreatic lipase inhibition assay was performed in vitro and confirmed by molecular docking analysis. 

Results: The F. racemosa fruit showed the highest pancreatic lipase inhibitory activity. Molecular docking studies also exhibited good binding affinity of compounds (Diethyl Phthalate) with pancreatic lipase enzymes. 

Conclusion: Finally, it is concluded that further derivation of such compounds could serve as the new templates for obesity.

Author Biographies

A. Vijaya Anand

Department of Human Genetics and Molecular Biology, 5Department of Biochemistry, Bharathiar University, Coimbatore, Tamil Nadu, India

V. Bharathi

Biological and Bioinformatics Research Centre, Trichy, Tamil Nadu, India

G. Bupesh

Department of Forest Science, Nagaland University, Lumami-798627, India

Jaya Lakshmi

Department of Biochemistry, Shrimati Indira Gandhi College, Trichy, Tamil Nadu, India

K. Meenakshi Sundaram

Department of Forest Science, Nagaland University, Lumami-798627, India

References

Strine, T. W., Mokdad, A. H., Balluz, L. S., Gonzalez, O., Crider, R., Berry. Depression and anxiety in the United States: findings from the 2006 Behavioral Risk Factor Surveillance System. Psychiatr Serv. 2008; 59(12): 1383-1390.

Padilla-Camberos, E., Flores-Fernandez, J. M., Fernandez-Flores, O., Gutierrez-Mercado, Y., Carmona-de la Luz, J. Sandoval-Salas,F., et al., Hypocholesterolemic effect and in vitro pancreatic lipase inhibitory activity of an Opuntia ficus-indica extract. BioMed Research International. 2015; 4.

Hill, J. O., Levine, J. S., Saris, W. H. M., Energy expenditure and physical activity. Handbook of Obesity. Second Edition Marcel Dekker, Inc; New York, N. Y. 2003; 631-654.

Lim, D. W., Song, M. I., Juyeon, K. P., Park, S. W., Kim, N. H., Gaire, B. P., et al., Anti-obesity effect of HT048., a herbal combination in high fat diet-induced obese rats. Molecules. 2012; 17: 14765-14767.

Arcari, D. P., Bartchewsky, W. Antiobesity effects of Yerba mate extract (Ilex paraguariensis) in high-fat diet–induced obese mice. Obesity. 2009; 17: 2127-2133.

Prats, E., Monfar, M., Castella, J. Energy intake of rats fed a cafeteria diet. Physiol Behavior. 1989; 45: 263-272.

Dhingra, D., Jindal, V., Sharma, S. Evaluation of antiobesity activity of Tinospora cordifolia stems in rats. Int J Res Ayurveda Pharm 2011; 2: 306-311.

Li, R. W., Leach, D. N., Myers, S. P., Lin, G. D., Leach, G. J., Waterman, P. G. A new anti-inflammatory glucoside from Ficus racemosa L. Planta Med. 2004; 70(5): 421-426.

Hall, K. D., Sacks, G., Ghandramohan, D. Quantifying the effect of energy imbalance on bodyweight Change. Lancet. 2011; 378: 826-837.

Sofowora, A. Medicinal Plants and Traditional Medicine in Africa. Spectrum Book Ltd., Ibadan, Nigeria. 1993; 191-289.

Harborne, J. B. Phytochemistry. Academic Press, London. 1993; 89-131.

Pramila, D. M., Xavier, R., Marimuthu, K., Kathiresan, S., Khoo, M. L., Senthilkumar, M., et al., 2012, Phytochemical analysisand antimicrobial potential ofmethanolic leafextract ofpeppermint (Mentha piperita: Lamiaceae), Journal of Medicinal Plants Research, Vol. 6(2), pp. 331-335.

Karpagasundari, C., Kulothungan, S. Analysis of bioactive compounds in Physalis minima leaves using GCMS, HPLC, UV-VIS and FTIR techniques. Journal of Pharmacognosy and Phytochemistry. 2014; 3(4): 196-201.

Mohammad, M., Aiedeh, K. M., Alkhatib, H. S., Tawaha, K., Alkhalidi,?B., Al-Masri, et al., Comparative enzymatic inhibition assay as a surrogate indicator of pharmaceutical and potency equivalence of two orlistat formulations. Jordan J. Pharm. Sci. 2010; 3: 69-75.

Meenakumari, K., Bupesh, G., Vasanth, S., Vasu, C. A., Pandian, K., Prabhu, K., et al., Molecular docking based virtual screening of carbonic anhydrase IX with coumarin (a cinnamon compound) derived ligands. Bioinformation. 2019; 15(10): 744-749.

Sahu, N., Saxena, J. Phytochemical analysis of Bougainvillea, Glabra choisy by ftir and uv-vis spectroscopic analysis. Int. J. Pharm. Sci. Rev. Res., 21(1), 2013; 33: 196-198.

Muruganatham, S., Anbalagan, G., and Ramamurthy, N. FT-IR and SEM-EDS Comparative analysis of medicinal plants, Eclipta alba Hask and Eclipta prostrata Linn. Romanian Journal of Biophysics. 2009; 19(4): 285-294.

G Bupesh, MS Nandini, S Vasanth, TS Vijayakumar, C Amutha, K Prabhu , 2019, Molecular modelling and docking analysis of pleurocidin (an antimicrobial peptide) like peptides with enterotoxin H from Klebsilla pneumonia, Bioinformation 2019; 15 (11), 838

Jagtap, A. P., Singh, N. P. Asclepiadaceae and periplocaceae. Fascicles of flora of India, Botanical Survey of India, Kolkata. Fascicle. 1999; 24: 211-241.

Minal, W., Ali, F., Agarwal, S., Despande, J., Mathew, S., Khetmalas, M. Qualitative phytochemical analysis and antimicrobial activity studies of Gymnema sylvestre. Acta Biologica Indica. 2012; 1(1): 121-124.

Saran, N., Anandharaj, B., Bupesh, G., Vasanth, S., Jasmine Beulah, J., Balachandar, V. Molecular docking analysis of a secondary metabolite with the glycoprotein receptors of HSV 1 and HSV 2. Bioinformation. 2019; 15(12): 887-895.

Penecilla, G. L., Magno, C. P. Antibacterial activity of extracts of twelve common medicinal plants from the Philippines. J Med Plants Res. 2011; (5): 3975-3981.

Indumathi, C. P., Bupesh, G., Vasanth, S., Senthilkumar, V., Anandh, A. V., Pandian, K. Molecular docking analysis of zanamavir with haem agglutinin neuraminidase of human para influenza virus type 3. Bioinformation. 2019; 15(10), 730-734.

Chimera resource for biocomputing visualization, and informatics (RBVI), San Francisco, CA, USA: National Institutes of Health; 2014. Available from: https://www.cgl.ucsf.edu/chimera/

Downloads

Published

2021-04-03

How to Cite

1.
Vijaya Anand A, Bharathi V, Bupesh G, Lakshmi J, Meenakshi Sundaram K, Saradhadevi . M. Identification of novel potent pancreatic lipase inhibitors from Ficus racemosa. Biomedicine [Internet]. 2021 Apr. 3 [cited 2024 Mar. 28];41(1):23-30. Available from: https://biomedicineonline.org/index.php/home/article/view/528

Issue

Section

Original Research Articles

Plum Analytics