Antibacterial activity of aquatic actinomycete,Actinoplanes digitatisDnj-2 (MN567713)

Authors

  • Ali Mohammed Bawazir
  • M. Jayashankar
  • Manjula Shantaram

DOI:

https://doi.org/10.51248/.v41i1.535

Keywords:

Actinomycetes, Actinoplanes digitatisDnj-2, antibacterial activities

Abstract

Introduction and Aim: There are centers for antibiotics in the nature to treat disease by looking for different sources, for example, actinomycetes, are approximately two-thirds of naturally occurring antibiotics, having much of medical importance, have been isolated from actinomycetes.The aim of the present study was to isolate and to identify the actinomycetes having antagonistic activity.

 

Materials and Methods: An actinomycetes strain isolated from dam soil sample collected from Alnajl dam of Hadhramout Yemen, showed antibacterial activity against selected microbial pathogens. The nutritional requirements and cultural conditions for maximal growth to produce secondary metabolites have been optimized under shake flask conditions. The growth and production of secondary metabolites were maximal with the use of SCA medium supplemented at pH 7.0, and incubation temperature and time of 37?and 20 days respectively.

 

Results:Based on morphological, biochemical, physiological and phylogenetic characterization, the strain was identified as Actinoplanes digitatisDnj-2 (MN567713). The ethyl acetate extract (1g/mL) obtained from the isolate showed significant antibacterial activity against Gram-negative bacteria- Escherichiacoli (21cm) and Pseudomonas aeruginosa (29cm) and Gram-positive bacteria- Bacillus subtitles(31cm) and Staphylococcus aureus (22cm) when compared with penicillin G 10 units.

 

Conclusion:In conclusion, the isolated strain has broad spectrum of antagonistic activity against Gram-positive and Gram-negativebacteria.

Author Biographies

Ali Mohammed Bawazir

Department of Studies and Research in Microbiology, Jnana Kaveri Post Graduate Centre, Mangalore University, Chikka Aluvara, Kodagu- 571232, Karnataka, India

M. Jayashankar

Department of Studies and Research in Microbiology, Jnana Kaveri Post Graduate Centre, Mangalore University, Chikka Aluvara, Kodagu- 571232, Karnataka, India

Manjula Shantaram

Department of Studies and Research in Biochemistry, Jnana Kaveri Post Graduate Centre, Mangalore University, Chikka Aluvara, Kodagu- 571232, Karnataka, India

References

Singh, S., Pelaez, F. Biodiversity, chemical and drug discovery. Progr. Drug Res. 2008; 65: 143-174.

Behal, V. Alternative sources of biologically active substances. Folia Microbiol. 2003; 48: 563-571.

Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. Hopwood, D. A.Practical Streptomyces Genetics (2nd ed.). Norwich, England: John Innes Foundation 2000.

Wynands, K., Van, P. H. A novel halogenase gene from the pentachloropseudilinproducer Actinoplanes sp. ATCC 33002 and detection of in vitrohalogenase activity. FEMS Microbiol. Lett 2004; 237: 363-367.

Holt, J. G., Krieg,N.R., Sneath, P. H. A., Staley, J.T.,Williams, S. T. Other genera. In: Bergey's manual of systematic. Bacteriology 1994; 9: 701-703.

Stackebrandt, E.,Kroppenstedt, R. Union of the genera Actinoplanes Couch, Ampulariella Couch, Amorphosporangium Couch in a redefined genus Actinoplanes. SystApplMicrobiol. 1987; 9: 110-114.

Phongsopitanun, W., Matsumoto, A., Inahashi, Y., Kudo, T., Mori, M., Shiomi, K., et al. Actinoplaneslichenis sp. nov., isolated from lichen. Int J Syst Evol Microbiol. 2016; 66:468-473.

Ngaemthao, W., Chunhametha, S.,Suriyachadkun, C.Actinoplanessubglobosus sp. nov., isolated from mixed deciduous forest soil.Int J SystEvolMicrobiol.2019; 66:4850-4855.

Marcone, G.L., Binda,E., Reguzzoni, M., Gastaldo, L., Dalmastri, C., Marinelli, F. Classification of Actinoplanes sp. ATCC 33076, an actinomycete that produces the glycolipodepsipeptide antibiotic ramoplanin, as Actinoplanesramoplaninifer sp. nov. Int J SystEvolMicrobiol. 2017; 67:4181-4188.

Qu, Z., Bao, X.D., Xie, Q.Y., Zhao, Y.X., Yan, B., Dai, H.F., et al.,Actinoplanessediminis sp. nov., isolated from marine sediment. Int J SystEvolMicrobiol. 2018; 68:71-75.

Couch, J. N. Some new genera and species of the Actinoplanaceae. J Elisha Mitchell Sci Soc. 1963; 79: 53–70.

Kanavade, V. L. Use of Bio-Industrial Waste for Production of Microbial Biomass with Potential in Environmental Management. Ph.D. Thesis, University of Pune, 2003; India.

Shirling, E. B., Gottlieb, D. Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol. 1966; 16: 312-340.

Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M., Sneath, P.H.,Sackin, M. J. Numerical classification of Streptomyces and related genera. J Gen Microbiol.1983; 129:1743-1813.

Williams, S. T., Wellington, E.M. H. Actinomycetes, Ch. In: Methods of Soil analysis. Part 2. Chemical and Microbiological Properties. 2nd edit. (Page, A. I. Miller, R. H. and Keeney, D. R. Eds.). Amer. Soc. Agronomy Soil Science Soc. America Inc. Pub. Madison, Wisconsin, USA. 1982; 45: 969-987.

Hsu, S. C., Lockwood, J. L. Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Applied Microbiology. 1975; 29:422-426.

Collins, C. H., Lyne, P. M., Granje, J. M. Microbiological Methods. Butterworth and Heinemann publishers 1995.

Deshmukh, A. L. Handbook of media, stains and reagent in microbiology. India:Pama Publication. 1997.

Healy, F. G., Lambert, D. H.Relationships among Streptomyces spp. causing potato scab.Int. J. Syst. Bacteriol. 1991; 41: 479-482.

Shejul, M. S. Studies on Heterotrophic Filamentous Procaryotes from Aquatic Habitats. Ph.D. Thesis, University of Pune, India. 1998.

Locci, R. Streptomycetes and related genera. Bergey's Manual of Systematic Bacteriology, Williams and Wilkins Company, Baltimore. 1989; 4: 2451-2508.

Williams, S. T., Goodfellow, M., Alderson, G. Genus Streptomyces Waksman and Henrici 1943, 399AL. In: Bergey's manual of systematic Bacteriology, vol. 4. (Williams, S. T. Sharpe, M. E. and Holt, J. G. Eds.),

Downloads

Published

2021-04-03

How to Cite

1.
Bawazir AM, Jayashankar M, Shantaram M. Antibacterial activity of aquatic actinomycete,Actinoplanes digitatisDnj-2 (MN567713). Biomedicine [Internet]. 2021Apr.3 [cited 2021Apr.14];41(1):65-9. Available from: https://biomedicineonline.org/index.php/home/article/view/535

Issue

Section

Original Research Articles