Human platelet-rich plasma as a biological stimulant for proliferation and differentiation of mesenchymal stem cells


  • Dewi Sukmawati
  • Helsy Junaidi
  • Rahimi Syaidah



Platelet-rich plasma, mesenchymal stem cells, cell culture, proliferation, differentiation, xeno-free


Platelet is one of the cells within the blood that have potential in regenerative therapy. Recently platelet-related products got special attention due to the abundance of growth factors and easy availability and processing. With the robust in cell-based therapy, platelet-rich plasma (PRP) has been intensively studied for its potential to substitute the fetal bovine serum (FBS) as the supplement in cell culture. Our study aimed to investigate the use of human PRP in mesenchymal stem cells (MSC) culture and its related effect on stem cell biology. We searched in vitro studies that used human PRP as a supplementing factor on human MSCs culture. From the initial 172 studies, 14 studies fulfilled the selection criteria and were analysed. The results showed that the sources of MSCs were varied, including adipose tissue, bone marrow, and dental tissue. The PRP concentration showing the best effects was ranged from 10 to 20%. In addition, most of the studies demonstrated the superiority of PRP to FBS in promoting the proliferation and differentiation of MSCs in vitro. Therefore, PRP could be an alternative to FBS in supporting a xeno-free culture system. Studies are needed to reveal the mechanism of PRP in maintaining the physiology of MSCs.

Author Biographies

Dewi Sukmawati

1) Department of Histology, 2) Doctoral Program in Biomedical Sciences, Faculty of Medicine, Unversitas Indonesia, Jln Salemba Raya No.4, Jakarta 10430

Helsy Junaidi

3) Postgraduate student of Doctoral Program in Biomedical Sciences, Faculty of Medicine, Unversitas Indonesia, Jln Salemba Raya No.4, Jakarta 10430.

Rahimi Syaidah

1) Department of Histology, 2) Doctoral Program in Biomedical Sciences, Faculty of Medicine, Unversitas Indonesia, Jln Salemba Raya No.4, Jakarta 10430.


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How to Cite

Sukmawati D, Junaidi H, Syaidah R. Human platelet-rich plasma as a biological stimulant for proliferation and differentiation of mesenchymal stem cells. Biomedicine [Internet]. 2021Jul.7 [cited 2021Jul.24];41(2):168-73. Available from: