Human platelet-rich plasma as a biological stimulant for proliferation and differentiation of mesenchymal stem cells

Authors

  • Dewi Sukmawati
  • Helsy Junaidi
  • Rahimi Syaidah

DOI:

https://doi.org/10.51248/.v41i2.777

Keywords:

Platelet-rich plasma, mesenchymal stem cells, cell culture, proliferation, differentiation, xeno-free

Abstract

Platelet is one of the cells within the blood that have potential in regenerative therapy. Recently platelet-related products got special attention due to the abundance of growth factors and easy availability and processing. With the robust in cell-based therapy, platelet-rich plasma (PRP) has been intensively studied for its potential to substitute the fetal bovine serum (FBS) as the supplement in cell culture. Our study aimed to investigate the use of human PRP in mesenchymal stem cells (MSC) culture and its related effect on stem cell biology. We searched in vitro studies that used human PRP as a supplementing factor on human MSCs culture. From the initial 172 studies, 14 studies fulfilled the selection criteria and were analysed. The results showed that the sources of MSCs were varied, including adipose tissue, bone marrow, and dental tissue. The PRP concentration showing the best effects was ranged from 10 to 20%. In addition, most of the studies demonstrated the superiority of PRP to FBS in promoting the proliferation and differentiation of MSCs in vitro. Therefore, PRP could be an alternative to FBS in supporting a xeno-free culture system. Studies are needed to reveal the mechanism of PRP in maintaining the physiology of MSCs.

Author Biographies

Dewi Sukmawati

1) Department of Histology, 2) Doctoral Program in Biomedical Sciences, Faculty of Medicine, Unversitas Indonesia, Jln Salemba Raya No.4, Jakarta 10430

Helsy Junaidi

3) Postgraduate student of Doctoral Program in Biomedical Sciences, Faculty of Medicine, Unversitas Indonesia, Jln Salemba Raya No.4, Jakarta 10430.

Rahimi Syaidah

1) Department of Histology, 2) Doctoral Program in Biomedical Sciences, Faculty of Medicine, Unversitas Indonesia, Jln Salemba Raya No.4, Jakarta 10430.

References

Srijaya, T. C., Ramasamy, T. S., Kasim, N. H. A. Advancing stem cell therapy from bench to bedside: lessons from drug therapies. J Transl Med [Internet]. 2014;12(243):1-17. Available from: http://translational-medicine.biomedcentral.com/articles/10.1186/s12967-014-0243-9

Dai, R., Wang, Z., Samanipour, R., Koo, K., Kim, K. Adipose-Derived Stem Cells for Tissue Engineering and Regenerative Medicine Applications. Stem Cells Int [Internet]. 2016; 1-19.

Brown, C., McKee, C., Bakshi, S., Walker, K., Hakman, E., Halassy, S., et al., Mesenchymal stem cells: Cell therapy and regeneration potential. J Tissue Eng Regen Med. 2019; 13(9): 1738-1755.

Dominici, M., Blanc, K. L., Mueller, I., Slaper-Cortenbach, I., Marini, F., Krause, D. S., et al., Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy [Internet]. 2006 Jan 1 [cited 2019 Dec 2]; 8(4): 315-317.

da Silva, M. L., Chagastelles, P. C., Nardi, N. B. Mesenchymal stem cells reside in virtually all post-natal organs and tissues. J Cell Sci [Internet]. 2006; 119(11): 2204-2213.

Kwon, A., Kim, Y., Kim, M., Kim, J. M. J., Choi, H., Jekarl, D. W., et al.,Tissue-specific Differentiation Potency of Mesenchymal Stromal Cells from Perinatal Tissues. Sci Rep [Internet]. 2016; 6(23544): 1-11.

Silva, G. V., Litovsky, S., Assad, J. A. R., Sousa, A. L. S., Martin, B. J., Vela, D., et al., Mesenchymal stem cells differentiate into an endothelial phenotype, enhance vascular density, and improve heart function in a canine chronic ischemia model. Circulation. 2005; 111(2): 150-156.

Cho, J., D’Antuono, M., Glicksman, M., Wang, J., Jonklaas, J. A review of clinical trials: mesenchymal stem cell transplant therapy in type 1 and type 2 diabetes mellitus. Am J Stem Cells [Internet]. 2018; 7(4): 82-93.

Ramezanifard, R., Kabiri, M., Ahvaz, H. H. Effects of platelet rich plasma and chondrocyte co-culture on msc chondrogenesis, hypertrophy and pathological responses. J Exp Clin Sci. 2017; 16: 1031-1045.

Sarfraz, A., Qureshi, A. S., Fakhar-I-Adil, Usman, M. Use of autologous platelet-rich plasma versus fetal bovine serum in mesenchymal stem cells culture media. EC Vet Sci. 2019; 2: 122-128.

Agostini, F., Polesel, J., Battiston, M., Lombardi, E., Zanolin, S., Da Ponte, A., et al., Standardization of platelet releasate products for clinical applications in cell therapy: a mathematical approach. J Transl Med [Internet]. 2017 Dec 19 [cited 2020 Nov 25]; 15(107): 1-10.

Van, P. P., Vu, N. B., Pham, V. M., Truong, N. H., Pham, T. L. B., Dang, L. T. T., et al., Good manufacturing practice-compliant isolation and culture of human umbilical cord blood-derived mesenchymal stem cells. J Transl Med [Internet]. 2014; 12(56): 1-10.

Raeissadat, S. A., Babaee, M., Rayegani, S. M., Hashemi, Z., Hamidieh, A. A., Mojgani, P., et al., An overview of platelet products in the Iranian studies. Futur Sci OA. 2017; 3(4): 1-15.

Kieb, M., Sander, F., Prinz, C., Adam, S., Mau-Möller, A., Bader, R., et al., Platelet-rich plasma powder: a new preparation method for the standardization of growth factor concentrations. Am J Sports Med. 2017; 45(4): 954-960.

Schär, M. O., Diaz-Romero, J., Kohl, S., Zumstein, M.A., Nesic, D. Platelet-rich Concentrates Differentially Release Growth Factors and Induce Cell Migration In Vitro. Clin Orthop Relat Res [Internet]. 2015 May 1 [cited 2020 Nov 26]; 473(5): 1635-1643.

Kleplová, T. S., Soukup, T., ?ehá?ek, V., Suchánek, J.

Human Plasma and Human Platelet-Rich Plasma As a Substitute for Fetal Calf Serum During Long-Term Cultivation of Mesenchymal Dental Pulp Stem Cells. ACTA MEDICA (Hradec Králové) [Internet]. 2014 Jan 27 [cited 2020 Nov 25]; 57(3): 119-126.

Vokurka, J., Gopfert, E., Blahutkova, M., Buchalova, E., Faldyna, M. Concentrations of growth factors in platelet-rich plasma and platelet-rich fibrin in a rabbit model. Vet Med (Praha). 2016; 61(10): 567-570.

Lubkowska, A., Dolegowska, B., Banfi, G. Growth factor content in PRP and their applicability in medicine. J Biol Regul Homeost Agents. 2012; 26(2 Suppl): 3S-22S.

Shiraishi, K., Kamei, N., Takeuchi, S., Yanada, S., Mera, H., Wakitani, S., et al., Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair. Stem Cells Int [Internet]. 2017; 2017: 1-9.

Lu, H., Wang, F., Mei, H., Wang, S., Cheng, L. Human adipose mesenchymal stem cells show more efficient angiogenesis promotion on endothelial colony-forming cells than umbilical cord and endometrium. Stem Cells Int [Internet]. 2018 [cited 2019 Mar 25]; 2018: 1-15.

Krafts, K. P. Tissue repair: The hidden drama [Internet]. Vol. 6, Organogenesis. Taylor & Francis; 2010 [cited 2020 Oct 29]. p. 225-233.

King, R. S., Newmark, P. A. The cell biology of regeneration. Journal of Cell Biology. The Rockefeller University Press; 2012; 196; 553-562.

Kondo, T. Timing of skin wounds. Leg Med. 2007; 9(2): 109-114.

Bretschneider, H., Quade, M., Lode, A., Gelinsky, M., Rammelt, S., Zwingenberger, S., et al., Characterization of naturally occurring bioactive factor mixtures for bone regeneration. Int J Mol Sci. 2020; 21(4): 1-15.

Conde-Montero, E., de la Cueva Dobao, P., González, M.J.M. Platelet-rich plasma for the treatment of chronic wounds: evidence to date. Chronic Wound Care Manag Res [Internet]. 2017; 4: 107-120.

Rosadi, I., Karina, K., Rosliana, I., Sobariah, S., Afini, I., Widyastuti, T., et al.,. In vitro study of cartilage tissue engineering using human adipose-derived stem cells induced by platelet-rich plasma and cultured on silk fibroin scaffold. Stem Cell Res Ther [Internet]. 2019 Dec 4 [cited 2020 Nov 26]; 10(1): 1-15.

de Melo, B. A. G., Luzo, Â. C. M., Lana, J. F. S. D., Santana, M. H. A. Centrifugation conditions in the L-PrP preparation affect soluble factors release and mesenchymal stem cell proliferation in fibrin nanofibers. Molecules [Internet]. 2019 [cited 2020 Nov 26]; 24(15): 1-14.

Aghajanova, L., Houshdaran, S., Balayan, S., Manvelyan, E., Irwin, J.C., Huddleston, H.G., et al., In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration. J Assist Reprod Genet [Internet]. 2018 Feb 5 [cited 2020 Nov 25]; 35(5): 757–70.

Wen, J., Li, H. T., Li, S. H., Li, X., Duan, J. M. Investigation of modified platelet-rich plasma (mPRP) in promoting the proliferation and differentiation of dental pulp stem cells from deciduous teeth. Brazilian J Med Biol Res [Internet]. 2016 [cited 2020 Nov 26]; 49(10): 1-8.

Suchánek, J., Kleplová, T.S., ?ehá?ek, V., Browne, K.Z., Soukup, T. Proliferative capacity and phenotypical alteration of multipotent ecto-mesenchymal stem cells from human exfoliated deciduous teeth cultured in xenogeneic and allogeneic media. Folia Biol (Czech Republic). 2016; 62(1): 1-14.

Ceci, C., Niada, S., Del Fabbro, M., Lolato, A., Taschieri, S., Giannasi, C., et al., Does freeze-thawing influence the effects of platelet concentrates? an in vitro study on human adipose-derived stem cells. J Craniofac Surg. 2016; 27(2): 398-404.

Atashi, F., Jaconi, M. E. E., Pittet-Cuénod, B., Modarressi, A. Autologous platelet-rich plasma: A biological supplement to enhance adipose-derived mesenchymal stem cell expansion. Tissue Eng - Part C Methods [Internet]. 2015 Mar 1 [cited 2020 Nov 25]; 21(3): 253-262.

Lee, J. K., Lee, S., Han, S. A., Seong, S. C., Lee, M. C. The effect of platelet-rich plasma on the differentiation of synovium-derived mesenchymal stem cells. J Orthop Res. 2014; 32(10): 1317-1325.

Amable, P. R., Teixeira, M. V. T., Carias, R. B. V., Granjeiro, J. M., Borojevic, R. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media. PLoS One [Internet]. 2014 Aug 12 [cited 2020 Nov 25];9(8): 1-8.

Tavakolinejad, S., Khosravi, M., Mashkani, B., Bideskan, A. E., Mossavi, N. S., Parizadeh, S. M. R., et al.,The effect of Human platelet-rich plasma on adipose-derived stem cell proliferation and osteogenic differentiation. Iran Biomed J [Internet]. 2014 [cited 2020 Nov 26];18(3): 150-156.

Cho, H. S., Song, I. H., Park, S. Y. Y., Sung, M. C., Ahn, M. W. W., Song, K. E. Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells. Korean J Lab Med [Internet]. 2011 Jul 1 [cited 2020 Nov 25]; 31(3): 212-218.

Sari, P., Luviah, E., Nugraha, Y., Pawitan, A.J.L., Purwoko, R.Y. Various growth factor yields in various platelet rich plasma processing methods. Biotechnology. 2010; 9(11): 475-478.

Pawitan, A. J. Platelet Rich Plasma in Xeno-Free Stem Cell Culture: The Impact of Platelet Count and Processing Method. Curr Stem Cell Res Ther. 2012; 7(5): 329-335.

Alves, R., Grimalt, R. A Review of Platelet-Rich Plasma: History, Biology, Mechanism of Action, and Classification. Ski Appendage Disord [Internet]. 2018; 18-24.

Dinescu, S., Ignat, S., Simion, T.V., Selaru, A., Samoila, I., Jianu, D., et al., Multiple benefits of platelet-rich plasma for regenerative medicine therapies. Rom Biotechnol Lett. 2018; 23(5): 13933-13939.

Lai, F., Kakudo, N., Morimoto, N., Taketani, S., Hara, T., Ogawa, T., et al., Platelet-rich plasma enhances the proliferation of human adipose stem cells through multiple signaling pathways. Stem Cell Res Ther [Internet]. 2018 Apr 16 [cited 2020 Dec 8]; 9(107): 1-10.

Elgamal, A., Asmaa, A. A., Abd-Elmaksoud, A., Kassab, M., Farag, A., Lashen, S., et al., Xeno-free trans-differentiation of adipose tissue-derived mesenchymal stem cells into glial and neuronal cells. Am J Stem Cells [Internet]. 2019; 8(2): 38-51.

Ng, F., Boucher, S., Koh, S., Sastry, K. S. R., Chase, L., Lakshmipathy, U., et al., PDGF, TGF-? And FGF signaling is important for differentiation and growth of mesenchymal stem cells (mscs): Transcriptional profiling can identify markers and signalig pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteo. Blood. 2008; 112(2): 295-307.

Van der Kraan, P. M., Blaney Davidson, E. N., Blom, A., van den Berg, W. B. TGF-beta signaling in chondrocyte terminal differentiation and osteoarthritis. Modulation and integration of signaling pathways through receptor-Smads. Osteoarthr Cartil. 2009 Dec 1; 17(12): 1539-1545.

Downloads

Published

2021-07-07

How to Cite

1.
Sukmawati D, Junaidi H, Syaidah R. Human platelet-rich plasma as a biological stimulant for proliferation and differentiation of mesenchymal stem cells. Biomedicine [Internet]. 2021 Jul. 7 [cited 2024 Mar. 29];41(2):168-73. Available from: https://biomedicineonline.org/index.php/home/article/view/777

Plum Analytics