Optimization and green synthesis of zinc oxide nanoparticle using Garcinia cambogia leaf and evaluation of their antioxidant and anticancer property in kidney cancer (A498) cell lines

Authors

  • Varsha Jayakar
  • Vinayak Lokapur
  • B. R. Nityasree
  • Raju Krishna Chalannavar
  • Lyned D. Lasrado
  • Manjula Shantaram

DOI:

https://doi.org/10.51248/.v41i2.785

Keywords:

Garcinia cambogia, green synthesis, zinc oxide nanoparticles, optimization, A498, HEK 293, renal cancer

Abstract

Introduction and Aim: In 21stcentury, nanomedicine has turned out to be an emergent modulus operation for the diagnosis and treatment of cancer. The current study includes the optimization and green synthesis of zinc oxide nanoparticles from the leaves of Garcinia cambogia and interpretation of its antioxidant and anticancer activity.

 

Materials and Methods: Synthesized ZnO NPs were investigated by UV–vis, FTIR, XRD, SEM, EDX, HR-TEM, SAED, particle size distribution and DLS analysis. Furthermore, different antioxidant assays were conducted for evaluating the antioxidant activity of (ZnO NPs) and its anticancer activity using (A498) cell lines along with non-cancerous (HEK-293) cell line using MTT assay and XTT assay.

 

Results: The UV–visible spectrum showed an absorption peak at 277 nm that reflects surface plasmon resonance (SPR) ZnONPs. The presence of various functional groups was confirmed by FTIR analysis. XRD patterns indicated that the ZnO NPs had a single-phase nature with the wurtzite structure. SEM, TEM pictures confirmed the size and shape of synthesized Nanoparticles ranges of 11–32 nm and mostly hexagonal forms. The Zeta potential value of 57.9 mV revealed the surface charge of green synthesized ZnONPs. The cytotoxicity of the ZnO NPs with various concentrations (6.25 to 200 µg/mL) was measured in (A498) cell lines along with non-cancerous (HEK-293) cell line. After the exposure, to MTT and XTT assay, cellular morphology studies showed that the cancer cell viability decreased with increasing ZnO NP concentration.

 

Conclusion: The synthesized ZnONPs via a green synthetic route could be developed as anti-cancer agent against cancer cells.

Author Biographies

Varsha Jayakar

Department of Studies and Research in Biochemistry, Jnana Kaveri PG Centre, ChikkaAluvara,Kodagu571 232, Karnataka, India

Vinayak Lokapur

Department of Studies and Research in Biochemistry, Jnana Kaveri PG Centre, ChikkaAluvara,Kodagu571 232, Karnataka, India

B. R. Nityasree

Department of Applied Botany, Mangalore University, Mangalagangothri, 574199 KarnatakaIndia

Raju Krishna Chalannavar

Department of Applied Botany, Mangalore University, Mangalagangothri, 574199 KarnatakaIndia

Lyned D. Lasrado

Department of Post Graduate Studies and Research in Biochemistry, St. Aloysius College (Autonomous), Mangalore, Karnataka, India

Manjula Shantaram

Department of Studies and Research in Biochemistry, Jnana Kaveri PG Centre, ChikkaAluvara,Kodagu571 232, Karnataka, India

References

Liu, R., Pei, Q., Shou, T., Zhang, W., Hu, J., Li, W. Apoptotic effect of green synthesized gold nanoparticles from Curcuma wenyujin extract against human renal cell carcinoma A498 cells. International Journal of Nanomedicine. 2019;14:4091.

Rutz, J., Maxeiner, S., Juengel, E., Bernd, A., Kippenberger, S., Zöller, N., et al. Growth and proliferation of renal cell carcinoma cells is blocked by low curcumin concentrations combined with visible light irradiation. International Journal of Molecular Sciences. 2019 Jan;20(6):1464.

Xu, M., Wang, Y., Zhou, L. N., Xu, L. J., Jin, Z. C., Yang, D. R., et al. The therapeutic value of SC66 in human renal cell carcinoma cells. Cell Death & Disease. 2020 May 11;11(5):1-1.

Swiatek, M., Jancewicz, I., Kluebsoongnoen, J., Zub, R., Maassen, A., Kubala, S.,et al. Various forms of HIF?1? protein characterize the clear cell renal cell carcinoma cell lines. IUBMB life. 2020 Jun;72(6):1220-1232.

Wang, X., Liu, J., Xie, Z., Rao, J., Xu, G., Huang, K.,et al. Chlorogenic acid inhibits proliferation and induces apoptosis in A498 human kidney cancer cells via inactivating PI 3K/Akt/mTOR signalling pathway. Journal of Pharmacy and Pharmacology. 2019;71(7):1100-1109.

Al-Ajmi, M. F., Hussain, A., Alsharaeh, E., Ahmed, F., Amir, S., Anwar, M. S., et al.Green synthesis of zinc oxide nanoparticles using Alstonia macrophylla leaf extract and their in-vitro anticancer activity. Science of Advanced Materials. 2018 Mar 1;10(3):349-355.

Selim, Y. A., Azb, M. A., Ragab, I., Abd El-Azim, M. H. Green synthesis of zinc oxide nanoparticles using aqueous extract of Deverratortuosa and their cytotoxic activities. Scientific Reports. 2020 Feb 26;10(1):1-9.

Seethapathy, G. S., Tadesse, M., Urumarudappa, S. K., Gunaga, S. V., Vasudeva, R., Malterud, K. E., et al. Authentication of Garcinia fruits and food supplements using DNA barcoding and NMR spectroscopy. Scientific Reports. 2018 Jul 12;8(1):1-2.

Jayakar, V., Lokapur, V., Shantaram, M. Identification of the volatile bioactive compounds by GC-MS analysis from the leaf extracts of Garcinia cambogia and Garcinia indica. Medicinal Plants-International Journal of Phytomedicines and Related Industries. 2020;12(4):580-590.

Banu, S., Ramakrishnaiah, T. N. Screening of Garcinia cambogia for in-vitroanti-cancerous activity against colon adenocarcinoma (Caco-2) cell line. Res. J. Pharmacognosy and Phytochem. 2018 Oct 31;10(4):272-276.

Hart, C., Cock, I. E. An examination of the antimicrobial and anticancer properties of Garcinia cambogia fruit pericarp extracts. Biology, Engineering, Medicine and Science Reports. 2016 Jul 1;2(2):55-63.

Espirito Santo, B. L., Santana, L. F., Kato Junior, W. H., de Araújo, F. D., Bogo, D., Freitas, K. D., et al. Medicinal potential of garcinia species and their compounds. Molecules. 2020 Jan;25(19):4513.

Sonia, S., Ruckmani, K., Sivakumar, M. Antimicrobial and antioxidant potentials of biosynthesized colloidal zinc oxide nanoparticles for a fortified cold cream formulation: a potent nanocosmeceutical application. Materials Science and Engineering: C. 2017 Oct 1;79:581-589.

Zare, E., Pourseyedi, S., Khatami, M., Darezereshki, E. Simple biosynthesis of zinc oxide nanoparticles using nature's source, and it's in vitro bio-activity. Journal of Molecular Structure. 2017 Oct 15;1146:96-103.

Singh, D., Rathod, V., Ninganagouda, S., Hiremath, J., Singh, A. K., Mathew, J. Optimization and characterization of silver nanoparticle by endophytic fungi Penicillium sp. isolated from Curcuma longa (turmeric) and application studies against MDR E. coli and S. aureus. Bioinorganic Chemistry and Applications. 2014 Jan 1;2014.

Lokapur, V., Jayakar, V., Shantaram, M. Preliminary phytochemical screening, physicochemical analysis and in-vitro antioxidant activity of selected Holigarnaspecies-Endemic plant species of Western Ghats. Biomedicine. 2020;40(4):460-466.

Sudha, K. G., Ali, S., Karunakaran, G., Kowsalya, M., Kolesnikov, E., Rajeshkumar, M. P. Eco?friendly synthesis of ZnO nanorods using Cycas pschannae plant extract with excellent photocatalytic, antioxidant, and anticancer nanomedicine for lung cancer treatment. Applied Organometallic Chemistry. 2020 Apr;34(4):e5511.

Rashidi, M., Seghatoleslam, A., Namavari, M., Amiri, A., Fahmidehkar, M. A., Ramezani, A., et al. Selective cytotoxicity and apoptosis-induction of Cyrtopodionscabrum extract against digestive cancer cell lines. International Journal of Cancer Management. 2017; 10(5): 7.

Malaikozhundan, B., Vaseeharan, B., Vijayakumar, S., Pandiselvi, K., Kalanjiam, M. A., Murugan, K.,et al. Biological therapeutics of Pongamia pinnata coated zinc oxide nanoparticles against clinically important pathogenic bacteria, fungi and MCF-7 breast cancer cells. Microbial Pathogenesis. 2017 Mar 1;104:268-277.

Revathi, M., Catharinsara, S., Manonmani, R., Claudio Medoona Rigley. Fourier transform infrared spectroscopic analysis of ethanolic leaf extract of Alternanthera sessilis (L.) and Trigonella foenum-graecum (L.). Indian Journal of Applied Research. 2019; 9(2): 45-46.

Iqbal, M. R., Asri, I. T., Rakhmah, S., Diantoro, M. Dielectric properties of TiO2 nanoparticles doped flavonoid extract of Pterocarpus indicuswilld (PIW) latex. InJournal of Physics: Conference Series 2018 Sep 1 (Vol. 1093, No. 1, p. 012042). IOP Publishing.

Hassen, H. H., Salim, E. T., Taha, J. M., Mahdi, R. O., Numan, N. H., Khalid, F. G.,et al. Fourier transform infrared spectroscopy and photo luminance results for Zno NPs prepared at different preparation condition using LP-PLA technique. International Journal of Nanoelectronics and Materials. 2018;11:65-72.

Chaudhary, R. G., Sonkusare, V. N., Bhusari, G. S., Mondal, A., Shaik, D. P., Juneja, H. D. Microwave-mediated synthesis of spinel CuAl 2 O 4 nanocomposites for enhanced electrochemical and catalytic performance. Research on Chemical Intermediates. 2018 Mar;44(3):2039-2060.

Gomathi, A. C., Rajarathinam, S. X., Sadiq, A. M., Rajeshkumar, S. Anticancer activity of silver nanoparticles synthesized using aqueous fruit shell extract of Tamarindus indica on MCF-7 human breast cancer cell line. Journal of Drug Delivery Science and Technology. 2020 Feb 1;55:101376.

Sethi, S., Joshi, A., Arora, B., Bhowmik, A., Sharma, R. R., Kumar, P. Significance of FRAP, DPPH, and CUPRAC assays for antioxidant activity determination in apple fruit extracts. European Food Research and Technology. 2020 Mar;246(3):591-598.

Prieto, P., Pineda, M., Aguilar, M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry. 1999 May 1;269(2):337-41.

Salari, S., Bahabadi, S. E., Samzadeh-Kermani, A., Yosefzaei, F. In-vitro evaluation of antioxidant and antibacterial potential of greensynthesized silver nanoparticles using Prosopisfarcta fruit extract. Iranian Journal of Pharmaceutical Research: IJPR. 2019;18(1):430.

Izuegbuna, O., Otunola, G., Bradley, G. Chemical composition, antioxidant, anti-inflammatory, and cytotoxic activities of Opuntiastricta cladodes. Plos one. 2019 Jan 29;14(1):e0209682.

Downloads

Published

2021-07-07

How to Cite

1.
Jayakar V, Lokapur V, Nityasree BR, Chalannavar RK, Lasrado LD, Shantaram M. Optimization and green synthesis of zinc oxide nanoparticle using Garcinia cambogia leaf and evaluation of their antioxidant and anticancer property in kidney cancer (A498) cell lines. Biomedicine [Internet]. 2021Jul.7 [cited 2021Jul.24];41(2):206-22. Available from: https://biomedicineonline.org/index.php/home/article/view/785

Issue

Section

Original Research Articles