Optimization and green synthesis of zinc oxide nanoparticle using Garcinia cambogia leaf and evaluation of their antioxidant and anticancer property in kidney cancer (A498) cell lines


  • Varsha Jayakar
  • Vinayak Lokapur
  • B. R. Nityasree
  • Raju Krishna Chalannavar
  • Lyned D. Lasrado
  • Manjula Shantaram




Garcinia cambogia, green synthesis, zinc oxide nanoparticles, optimization, A498, HEK 293, renal cancer


Introduction and Aim: In 21stcentury, nanomedicine has turned out to be an emergent modulus operation for the diagnosis and treatment of cancer. The current study includes the optimization and green synthesis of zinc oxide nanoparticles from the leaves of Garcinia cambogia and interpretation of its antioxidant and anticancer activity.


Materials and Methods: Synthesized ZnO NPs were investigated by UV–vis, FTIR, XRD, SEM, EDX, HR-TEM, SAED, particle size distribution and DLS analysis. Furthermore, different antioxidant assays were conducted for evaluating the antioxidant activity of (ZnO NPs) and its anticancer activity using (A498) cell lines along with non-cancerous (HEK-293) cell line using MTT assay and XTT assay.


Results: The UV–visible spectrum showed an absorption peak at 277 nm that reflects surface plasmon resonance (SPR) ZnONPs. The presence of various functional groups was confirmed by FTIR analysis. XRD patterns indicated that the ZnO NPs had a single-phase nature with the wurtzite structure. SEM, TEM pictures confirmed the size and shape of synthesized Nanoparticles ranges of 11–32 nm and mostly hexagonal forms. The Zeta potential value of 57.9 mV revealed the surface charge of green synthesized ZnONPs. The cytotoxicity of the ZnO NPs with various concentrations (6.25 to 200 µg/mL) was measured in (A498) cell lines along with non-cancerous (HEK-293) cell line. After the exposure, to MTT and XTT assay, cellular morphology studies showed that the cancer cell viability decreased with increasing ZnO NP concentration.


Conclusion: The synthesized ZnONPs via a green synthetic route could be developed as anti-cancer agent against cancer cells.

Author Biographies

Varsha Jayakar

Department of Studies and Research in Biochemistry, Jnana Kaveri PG Centre, ChikkaAluvara,Kodagu571 232, Karnataka, India

Vinayak Lokapur

Department of Studies and Research in Biochemistry, Jnana Kaveri PG Centre, ChikkaAluvara,Kodagu571 232, Karnataka, India

B. R. Nityasree

Department of Applied Botany, Mangalore University, Mangalagangothri, 574199 KarnatakaIndia

Raju Krishna Chalannavar

Department of Applied Botany, Mangalore University, Mangalagangothri, 574199 KarnatakaIndia

Lyned D. Lasrado

Department of Post Graduate Studies and Research in Biochemistry, St. Aloysius College (Autonomous), Mangalore, Karnataka, India

Manjula Shantaram

Department of Studies and Research in Biochemistry, Jnana Kaveri PG Centre, ChikkaAluvara,Kodagu571 232, Karnataka, India


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How to Cite

Jayakar V, Lokapur V, Nityasree BR, Chalannavar RK, Lasrado LD, Shantaram M. Optimization and green synthesis of zinc oxide nanoparticle using Garcinia cambogia leaf and evaluation of their antioxidant and anticancer property in kidney cancer (A498) cell lines. Biomedicine [Internet]. 2021Jul.7 [cited 2021Jul.24];41(2):206-22. Available from: https://biomedicineonline.org/index.php/home/article/view/785



Original Research Articles