Biosynthesis and characterization of silver nanoparticles using Cinnamomum zeylanicum extract anda study of antibacterial effect against multi-drug resistance Gram-negativebacteria

Authors

  • Saleh Reyam F.
  • Gaidan Ayad M

DOI:

https://doi.org/10.51248/.v41i2.791

Keywords:

Silver nanoparticles, biosynthesis, bacteria

Abstract

Introduction and Aim:Nanoparticles for some metals can be used in the treatment of diseases caused by different pathogenic bacteria that are resistant to antibiotics due to the antibacterial properties of thesenanoparticles.In the current study, thesynthesis of silver nanoparticles (Ag) from Cinnamon zeylanicum bark extractwas investigated.

 

Materials and Methods:One mL of cinnamon bark extract was added to 50mL of 1mM of the silver nitrate (AgNO3). After incubation time of bark extract with AgNO3at room temperature for 1-18hours, the silver nanoparticles synthesis through changed colour of the mixture to dark brown.The UV, TEM and FT-IR analysis were carried out to characterize the biosynthesized Ag-NPs, UV-Vis scan showed absorption around 435 nm while TEM showedAg-NPs spherical shape and the sizes of the and FT-IR spectrum detected the presence of different functional groups responsible for reduction and stability of Ag-NPs in an aqueous solution.

 

Results:Biosynthesized Ag-NPs showed inhibitory effect against someGram-negative bacterial species that have complete resistance to the antibiotics P, NA, VA, E, TE. The inhibition zone of Ag-NPs wasdetermined as follows (Citrobacter freundii19mm, Klebsiella pneumonae23mm, E.coli 23mm, Enterobacter spp 20 mm, Acinetobacter baumannii20mm.Therefore, Ag-NPs can be used as alternative treatment for some antibiotics.

 

Conclusion: The Ag-NPs can be successfully prepared form Cinnamomum bark plant. Besides their low cost, these NPs had an antibacterial effect especially against Gram negative bacteria which had a complete resistance to the most common antibiotics

Author Biographies

Saleh Reyam F.

Department of Microbiology, College of Science, Tikrit University, Tikrit, Iraq

Gaidan Ayad M

Department of Microbiology, College of Science, Tikrit University, Tikrit, Iraq

References

Fair, R. J., Tor, Y. Antibiotics and bacterial resistance in the 21st century. Perspectives in medicinal chemistry. 2014; 6: PMC-S14459.

Meštrovi?, T., Ljubin-Sternak, S. Molecular mechanisms of Chlamydia trachomatis resistance to antimicrobial drugs. Frontiers in Bioscience. 2018; 23: 656-670.

El-Argawy, E., Rahhal, M. M. H., El-Korany, A., Elshabrawy, E. M., Eltahan, R. M. Efficacy of some nanoparticles to control damping-off and root rot of sugar beet in El-Behiera Governorate. Asian J Plant Pathol. 2017; 11: 35-47.

Kumar, P. V., Pammi, S. V. N., Kollu, P., Satyanarayana, K. V. V., Shameem, U.Green synthesis and characterization of silver nanoparticles using Boerhaaviadiffusa plant extract and their antibacterial activity. Industrial Crops and Products. 2014; 52: 562-566.

Yang, Y., Jin, P., Zhang, X., Ravichandran, N., Ying, H., Yu, C., et al., New epigallocatechin gallate (EGCG) nanocomplexes co-assembled with 3-mercapto-1-hexanol and ?-lactoglobulin for improvement of antitumor activity. Journal of Biomedical Nanotechnology.2017; 13(7): 805-814.

Devi, M., Devi, S., Sharma, V., Rana, N., Bhatia, R. K., Bhatt, A. K., et al., Green synthesis of silver nanoparticles using methanolic fruit extract of Aegle marmelos and their antimicrobial potential against human bacterial pathogens. Journal of traditional and complementary medicine. 2020; 10(2): 158-165.

Elshawy, O.E., Helmy, E.A., Rashed, L.A.Preparation, characterization and in vitro evaluation of the antitumor activity of the biologically synthesized silver nanoparticles. Adv. Nanopart. 2016; 5: 149-166.

Tanase, C., Berta, L., Coman, N. A., Ro?ca, I., Man, A., Toma, F., et al., Investigation of in vitro antioxidant and antibacterial potential of silver nanoparticles obtained by biosynthesis using beech bark extract. Antioxidants. 2019; 8(10): 459.

Ferreres, F., Gomes, N. G., Valentão, P., Pereira, D. M., Gil-Izquierdo, A., Araújo, L. et al., Leaves and stem bark from Allophylus africanus P. Beauv. An approach to anti-inflammatory properties and characterization of their flavonoid profile. Food and Chemical Toxicology. 2018; 118: 430-438.

Chanda, N., Shukla, R., Zambre, A., Mekapothula, S., Kulkarni, R. R., Katti, K., et al., An effective strategy for the synthesis of biocompatible gold nanoparticles using cinnamon phytochemicals for phantom CT imaging and photoacoustic detection of cancerous cells. Pharmaceutical research. 2011; 28(2): 279-291.

Shankar, S.S.; Ahmad A., Sastry, M.Geranium leaf assisted biosynthesis of silver nanoparticles, Biotechnol. Prog. 19 1627–1631.nanoparticles, Langmuir. 2003; 19: 1357-1361.

Vandepitte, J.; Engbaek, k.; Rohner, P.; Piot, P., Heuck, C. C.Basic Laboratory Procedures in Clinical Bacteriology. World Health organization. 2003; Geneva.

Sathishkumar, M.; Sneha, K.; Won, S. W.; Cho, C. W.; Kim, S., Yun, Y. S.Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surf Biointerfaces. 2009; 73(2): 332-338.

Gauthami, M., Srinivasan, N., M Goud, N., Boopalan, K., Thirumurugan, K. Synthesis of silver nanoparticles using Cinnamomum zeylanicum bark extract and its antioxidant activity. Nanoscience & Nanotechnology-Asia. 2015; 5(1): 2-7.

Saleh, R. F. Gaidan, A. M. Al-Mayah, Q. S. Green Synthesis of silver nanoparticles using aqueous leaf extract of Ocimumbasilicumand investigation of their potential antibacterial activity. Trop J Nat Prod Res. 2021; 5(1):94-99.

Hamzah, H. M. Saleh, R. F.,Maroof, M. N.Fusarium mangiferae as New Cell Factories for Producing Silver Nanoparticles. J. Microbiol. Biotechnol. 2018; 28(10): 1654-1663.

Namasivayam,S.J.K.R.Jayakumar,D.,Kumar,R.,Bharani,R.S.A. Antibacterial and anticancerous biocompatible silver nanoparticles synthesized from the cold-tolerant strain of Spirulina platensis. Journal of Coastal Life Medicine. 2015; 3(4):265-262.

Saliem, A. H., Ibrahim, O. M., Salih, S. I. Biosynthesis of silver nanoparticles using Cinnamon zeylanicum plants bark extract. kufa journal for Veterinary Medical Sciences. 2016; 7(1): 51-63.

S.& K. Srinivasan, Antioxidant and antimicrobial activity of silver nanoparticles synthesized using Cinnamomum zeylanicum. IAJPS. 2017; 4(01): 8-12.

Kumararaja, G., Sundaraganapathy, R., Constantine, I., Vijayalakshmi, V., Rahim, S. F. A.Comparative studies on synthesized silver nanoparticles using Artemisia vulgaris Linn., and Cinnamomum zeylanicumNees., for their antifungal activity. Journal of Pharmaceutical Sciences and Research. 2019; 11(7): 2558-2565.

Almalah, H. I., Alzahrani, H. A.,Abdelkader, H. S. Green Synthesis of Silver Nanoparticles using Cinnamomum Zylinicumand their Synergistic Effect against Multi-Drug Resistance Bacteria. Journal of Nanotechnology Research. 2019; 1(3): 95-107.

Adebayo-Tayo,A.,B., Salaam,A., Ajibade,A.Green synthesis of silver nanoparticle using Oscillatoria sp. extract, its antibacterial, antibiofilm potential and cytotoxicity activity.Heliyon. 2019; 5(2): 1-8

Wypij, M.; Czarnecka, J.; ?wiecimska, M.; Dahm, H.; Rai, M.; Golinska, P., et al., Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensisOF1 strain. World J. Microbial. Biotechnol. 2018; 34:23.

Ibrahim, O.M.S., Saliem, A.H., Salih, S.I. Antibacterial activity of silver nanoparticles synthesized by Cinnamon zeylanicumbark extract against Staphylococcus aureus. Al-Anbar J. Vet. Sci. 2016; 9(1): 22-36.

Saleh, T.H., Hashim, S.T., Malik, S.N., AL-Rubaii, B.A.L. Down-Regulation of flilgene expression by Ag nanoparticles andTiO2 nanoparticles in pragmatic clinical isolates of proteus mirabilis and proteus vulgaris from urinary tract infection. Nano Biomed Eng. 2019; 11(4): 321-332.

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Published

2021-07-07

How to Cite

1.
Reyam F. S, Ayad M G. Biosynthesis and characterization of silver nanoparticles using Cinnamomum zeylanicum extract anda study of antibacterial effect against multi-drug resistance Gram-negativebacteria. Biomedicine [Internet]. 2021Jul.7 [cited 2021Jul.24];41(2):249-55. Available from: https://biomedicineonline.org/index.php/home/article/view/791

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Original Research Articles