Novel urinary markers: taurine, dopamine and L-fucose levels in predicting neonatal seizures

Authors

  • Varashree B. S. Department of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104
  • Sravya Poduri Department of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104
  • Leslie Edward Lewis Department of Paediatrics, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104
  • Vijetha Shenoy Belle Department of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104

DOI:

https://doi.org/10.51248/.v41i4.877

Keywords:

Gamma amino butyric acid, neuromodulator, neonatal seizure, fucose, doapmine, taurine

Abstract

Introduction and Aim: Neonatal seizure is an age specific neurological emergency. Their unique pathophysiological mechanism has become subject of interest for many research studies. The recurrence risk for seizures is high during neonatal period and currently used treatment strategies have limited efficacy in preventing it. From past decades although the treatment has not changed, there is a gradual progress in various mechanisms that are involved in generation of seizures and their response to anti-epileptics. With the emergence of new biochemical parameters for risk assessment in patients with seizures, there is a strong need for their comparative evaluation in order to evaluate their potential clinical application. So, this study was carried out to compare the urine levels of taurine, dopamine and fucose in assessing their role in mechanism of seizure.

 

Materials and Methods: After obtaining ethical approval and consent from parents total 43 neonates, urine taurine, dopamine and fucose were measured in 24 cases of seizures and 19 apparently healthy normal controls. Dopamine and Taurine were measured using ELISA and L-fucose by Dische and Shettles method.

 

Results: The median level of urine fucose was significantly higher in male neonates, taurine was significantly decreased in cases compared to that of controls. Males had higher preponderance to develop seizures. The median levels of urine dopamine were high in cases compared to controls but has not showed any significance.

 

Conclusion: Amino acid like taurine, carbohydrate moiety like fucose and a neuromodulator like dopamine may have a mechanistic role in development of seizures in neonatal period.

References

Marques, A. H., O'Connor, T. G., Roth, C., Susser, E., Bjørke-Monsen, A. L. The influence of maternal prenatal and early childhood nutrition and maternal prenatal stress on offspring immune system development and neurodevelopmental disorders. Front Neurosci. 2013; 31(7): 120.

Basavarajappa, B. S. Neuropharmacology of the Endocannabinoid Signaling System-Molecular Mechanisms, Biological Actions and Synaptic Plasticity. Curr Neuropharmacol. 2007; 5(2): 81-97.

Desforges, M., Ditchfield, A., Hirst, C. R., Pegorie, C., Martyn-Smith, K., Sibley, C. P., et al., Reduced placental taurine transporter (TauT) activity in pregnancies complicated by pre-eclampsia and maternal obesity. Adv Exp Med Biol. 2013; 776: 81-91.

Desforges, M., Parsons, L., Westwood, M., Sibley, C. P., Greenwood, S. L. Taurine transport in human placental trophoblast is important for regulation of cell differentiation and survival. Cell Death and Disease. 2013; 4: e559.

Mosley, R. L., Benner, E. J., Kadiu, I., Thomas, M., Boska, M. D., Hasan, K., et al., Neuroinflammation, oxidative stress and the pathogenesis of parkinson's disease. Clin Neurosci Res. 2006; 6(5): 261-281.

Hagberg, H., Mallard, C., Ferriero, D. M., Vannucci, S. J., Levison, S. W., Vexler, Z. S., et al., The role of inflammation in perinatal brain injury. Nat Rev Neurol. 2015; 11(4): 192-208.

Johnston, M. V. Excitotoxicity in perinatal brain injury. Brain Pathol. 2005; 15(3): 234-240.

Okazaki, K., Nishida, A., Kimura, H. Inflammatory Mediators in Neonatal Asphyxia and Infection. In: Buonocore G., Bracci R., Weindling M. (eds) Neonatology. Springer, Cham. 2016; https://doi.org/10.10 07/978-3-319-18159-2_248-1

Alvarez-Díaz, A., Hilario, E., de Cerio, F. G., Valls-i-Soler, A., Alvarez-Díaz, F. J. Hypoxic-ischemic injury in the immature brain--key vascular and cellular players. Neonatology. 2007; 92(4): 227-235.

Hablitz, J. J. Regulation of circuits and excitability: implications for epileptogenesis. Epilepsy Curr. 2004; 4(4): 151-153.

Scharfman, H. E. The neurobiology of epilepsy. Curr Neurol Neurosci Rep. 2007; 7(4): 348-354.

Maverakis, E., Kim, K., Shimoda, M., Gershwin, M. E., Patel, F., Wilken, R., et al., Glycans in the immune system and The Altered Glycan Theory of Autoimmunity: a critical review. J Autoimmun. 2015; 57: 1-13.

Kumar, S., Saxena, M., Srinivas, K., Singh, V. K. Fucose: A biomarker in grading of oral cancer. Natl J Maxillofac Surg. 2015; 6(2): 176-179.

Yager, J. Y., Armstrong, E. A., Miyashita, H., Wirrell, E. C. Prolonged neonatal seizures exacerbate hypoxic-ischemic brain damage: correlation with cerebral energy metabolism and excitatory amino acid release. Dev Neurosci. 2002; 24(5): 367-381.

Lucassen, P. J., Pruessner, J., Sousa, N., Almeida, O. F., Van Dam, A. M., Rajkowska, G., et al., Neuropathology of stress. Acta Neuropathol. 2014; 127(1): 109-135.

Shimoda, L. A., Polak, J. Hypoxia. 4. Hypoxia and ion channel function. Am J Physiol Cell Physiol. 2011; 300(5): C951-C967.

Buckler, K. J., Vaughan-Jones, R. D., Peers, C., Nye, P. C. Intracellular pH and its regulation in isolated type I carotid body cells of the neonatal rat. J Physiol. 1991; 436: 107-129.

Gu, X. Q., Haddad, G. G. Decreased neuronal excitability in hippocampal neurons of mice exposed to cyclic hypoxia. J Appl Physiol (1985). 2001; 91(3): 1245-1250.

Banasiak, K. J., Burenkova, O., Haddad, G. G. Activation of voltage-sensitive sodium channels during oxygen deprivation leads to apoptotic neuronal death. Neuroscience. 2004; 126(1): 31-44.

Bernhardt, W. M., Warnecke, C., Willam, C., Tanaka, T., Wiesener, M. S., Eckardt, K. U. Organ protection by hypoxia and hypoxia-inducible factors. Methods Enzymol. 2007; 435: 221-245.

Le Magueresse, C., Monyer, H. GABAergic interneurons shape the functional maturation of the cortex. Neuron. 2013; 77(3): 388-405.

Chepkova, A. N., Sergeeva, O. A., Haas, H. L. Long-lasting enhancement of corticostriatal transmission by taurine: role of dopamine and acetylcholine. Cell Mol Neurobiol 2005; 25: 767-776.

Bozzi, Y., Borrelli, E. The role of dopamine signaling in epileptogenesis. Front Cell Neurosci. 2013; 7:157.

Lubrich, B., van Calker, D. Inhibition of the high affinity myo-inositol transport system: a common mechanism of action of antibipolar drugs? Neuropsychopharmacol. 1999; 21: 519-529.

Einat, H., Kofman, O., Itkin, O., Lewitan, R., Belmaker, R. H. Augmentation of lithium's behavioral effect by inositol uptake inhibitors. J Neural Transm 1998; 105: 31-38.

Wolfson, M., Bersudsky, Y., Hertz, E., Berkin, V., Zinger, E., Hertz, L. A Model of Inositol Compartmentation in Astrocytes Based Upon Efflux Kinetics and Slow Inositol Depletion after Uptake Inhibition Neurochemical Research 2000; 25(7): 977-998.

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Published

2021-12-31

How to Cite

1.
B. S. V, Poduri S, Lewis LE, Shenoy Belle V. Novel urinary markers: taurine, dopamine and L-fucose levels in predicting neonatal seizures. Biomedicine [Internet]. 2021Dec.31 [cited 2022Jan.20];41(4):732-6. Available from: https://biomedicineonline.org/index.php/home/article/view/877

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Original Research Articles