Antibacterial efficacy of Lawsonia inermis (Henna) against the predominant endodontic pathogen (Enterococcus faecalis)-An in vitro antimicrobial assay

Authors

  • J.A. Amilia Elizabeth
  • R. Hemalatha
  • P. Shankar
  • Trophimus Gnanabagyan Jayakaran
  • M. Rajkumar

DOI:

https://doi.org/10.51248/.v42i2.997

Keywords:

Lawsonia inermis, irrigants, antimicrobial activity, zone of inhibition, Enterococcus faecalis

Abstract

Introduction and Aim: The prospect of a successful root canal therapy relies on three main factors which includes proper instrumentation, disinfection and obturation of root canal. The goal of the present study is to analyse the anti-microbial efficacy of henna (Lawsonia inermis) against the major endodontic pathogen, Enterococcus faecalis.

 

Materials and Methods: In this study, the antibacterial efficacy of Lawsonia inermis is investigated against Enterococcs feacalis using the method of agar disc diffusion. Extracts of L. inermis were prepared by using methanol as an extraction solvent, whereas DMSO (Dimethyl sulfoxide) and water were used as dissolution solvents. The diluted henna sample was used as the test sample, while positive control used was chlorhexidine gluconate solution and the negative control used was saline. The bacteria were cultured, and the samples were placed to measure the zone of inhibition.

 

Results: Extracts of L. inermis displayed noteworthy antimicrobial activity against E. fecalis. The zone of inhibition of henna against E. fecalis was found to be 11mm whereas the positive control had 14mm.

 

Conclusion: In conclusion, the henna extracts had shown acceptable antimicrobial efficacy and thus this study provides the scientific reason for the use of L. inermis in dentistry.

Author Biographies

J.A. Amilia Elizabeth

Department of Pediatric and Preventive Dentistry, SRM Dental College and Hospital, Ramapuram, Chennai 600089, Tamil Nadu, India

R. Hemalatha

Department of Pediatric and Preventive Dentistry, SRM Dental College and Hospital, Ramapuram, Chennai 600089, Tamil Nadu, India

P. Shankar

Department of Pediatric and Preventive Dentistry, SRM Dental College and Hospital, Ramapuram, Chennai 600089, Tamil Nadu, India

Trophimus Gnanabagyan Jayakaran

Department of Pediatric and Preventive Dentistry, SRM Dental College and Hospital, Ramapuram, Chennai 600089, Tamil Nadu, India

M. Rajkumar

Department of Pediatric and Preventive Dentistry, SRM Dental College and Hospital, Ramapuram, Chennai 600089, Tamil Nadu, India

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Published

2022-05-01

How to Cite

1.
Elizabeth JA, Hemalatha R, Shankar P, Jayakaran TG, Rajkumar M. Antibacterial efficacy of Lawsonia inermis (Henna) against the predominant endodontic pathogen (Enterococcus faecalis)-An in vitro antimicrobial assay. Biomedicine [Internet]. 2022 May 1 [cited 2022 Nov. 27];42(2):401-4. Available from: https://biomedicineonline.org/home/article/view/997

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