Biomedicine https://biomedicineonline.org/index.php/home <p>Biomedicine (Print ISSN: 0970 2067), official publication of Indian Association of Biomedical Scientists (IABMS), published quarterly in March, June, September and December. It is an International Journal for Biomedical Sciences. The journal publishes research papers, reviews, special article, case report, book review and letter to the editor related to Anatomy, Physiology, Biochemistry, Microbiology, Toxicology, Endocrinology, Reproductive Biology, Pharmacology and Alternative Systems of Medicines like Siddha, Unani, Ayurveda, Homeopathy and Naturopathy. Preference will be given to papers of immediate importance to other investigators, either by their experimental data, new ideas or new methodology. Scientific correspondence to the Editor-in-Chief related to the published papers may also be accepted provided that they are short and scientifically relevant to the papers mentioned, in order to provide a continuing forum for discussion.</p> <p> </p> <p><strong>Abstracting and Indexing</strong></p> <p>Abstract and Articles are indexed in various databases, such as,</p> <p><a href="https://www.scopus.com/sourceid/28618#tabs=2"><strong>Scopus</strong></a></p> <p><strong>Excerpta Medica</strong></p> <p><strong>Google Scholar</strong></p> <p><strong>Elsevier Indian Citation Index</strong></p> <p><strong>Index Medicus,</strong></p> <p><strong>UGC- CARE</strong></p> <p><strong>NLM Unique ID: 8405231</strong>.</p> <p>Meta Data deposited in DOI and citation is tracked by<strong> crossref.</strong></p> en-US editor-in-chief@biomedicineonline.org (Prof.Manjula Shantaram) support@biomedicineonline.org (Journal Manager) Sun, 01 May 2022 00:00:00 +0800 OJS 3.2.1.1 http://blogs.law.harvard.edu/tech/rss 60 Biomarkers of secondary inflammation following intracerebral hemorrhage studied using adult Zebrafish model https://biomedicineonline.org/index.php/home/article/view/1923 <p>Intracerebral hemorrhage is the condition of bleeding inside the brain, either spontaneous or traumatic. Though this form of stroke accounts to only about 15% of the cases, the mortality rate is the highest. People with stroke associated with ICH very rarely regain functional independence. The post-stroke complications are due to the extending loss of vascular integrity leading to prolonged secondary inflammation. Early detection of biomarkers associated with the condition and the efficacy of these biomarkers to depict the prognostic rate become essential tools for the clinicians to handle patients with ICH. Development of <em>in vivo</em> models that can simulate the intricate pathophysiology of the condition can add up new biomarkers that serve diagnostic and prognostic purposes.</p> Lalitha Vaidyanathan, Madhan Kumar Srinivasan Copyright (c) https://biomedicineonline.org/index.php/home/article/view/1923 Serum Fatty Acid Transport Protein 1 in Women with and without Breast Cancer https://biomedicineonline.org/index.php/home/article/view/1920 <p><strong>Introduction and Aim:</strong>Obesity influences the serum lipid profiles leading to dysregulation of lipid metabolism in which fatty acid transporters like fatty acid transport protein (FATP1) are known to play a crucial role in the progression of breast cancer (BC). The study aims to compare FATP1 levels in women with and without &nbsp;BC.</p> <p><strong>Materials and Methods</strong>:In this case-control study, 50 BC and 50 control subjects were recruited based on the inclusion and exclusion criteria. Baseline characteristics such as height, weight, age and BMI were noted for all the subjects. Lipid profile was analysed using a commercially available kit. The serum level of FATP1 was estimated using a commercially available kit. SPSS version 22 was used to analyze the data, P&lt;0.05 was considered statistically significant.</p> <p><strong>Results:</strong>The serum FATP1 levels in subjects with BC differed significantly between the case and control. The total cholesterol (TC), triglycerides (TG), HDL-C, LDL-C and VLDL-C differed between the two groups and were statistically significant. Further, a comparison of serum FATP1 levels in subjects with BC based on receptor status showed a statistically significant difference in TG, VLDL-C and serum FATP1. Correlating the serum FATP1 levels with baseline characteristics and lipid profiles in subjects with BC, age showed a positive correlation with serum FATP1 levels. Correlation of FATP1 with different receptor status in subjects with BC showed a positive correlation between FATP1 and VLDL in ER+/PR-/HER2+ receptor status, and FATP1 and HDL-C showed a positive correlation in triple-negative breast cancer.</p> <p><strong>Conclusion:</strong>Though extensive research on BC is underway, study reports on serum FATP1 levels in BC are scarce. This study demonstrates the importance of serum levels of FATP1 in BC. Further study on the molecular regulation of FATP1 in BC will add to the knowledge, and findings of the study implicates for utilizing FATP1 as a biomarker in BC.</p> RANJITHA Acharya Copyright (c) https://biomedicineonline.org/index.php/home/article/view/1920 Serum Fatty Acid Transport Protein 1 in Women with and without Breast Cancer https://biomedicineonline.org/index.php/home/article/view/1916 <p><strong>Introduction and Aim:</strong>Obesity influences the serum lipid profiles leading to dysregulation of lipid metabolism in which fatty acid transporters like fatty acid transport protein (FATP1) are known to play a crucial role in the progression of breast cancer (BC).The study aims to compare FATP1 levels in women with and without &nbsp;BC.</p> <p><strong>Materials and Methods</strong>: In this case-control study, 50 BC and 50 control subjects were recruited based on the inclusion and exclusion criteria. Baseline characteristics such as height, weight, age and BMI were noted for all the subjects. Lipid profile was analysed using commercially available kit. The serum level of FATP1 was estimated using a commercially available kit. SPSS version 22 was used to analyze the data, P&lt;0.05 was considered statistically significant.</p> <p><strong>Results:</strong> The serum FATP1 levels in subjects with BC differed significantly between the case and control. The total cholesterol (TC), triglycerides (TG), HDL-C, LDL-C and VLDL-C differed between the two groups and were statistically significant. Further, a comparison of serum FATP1 levels in subjects with BC based on receptor status showed a statistically significant difference in TG, VLDL-C and serum FATP1.Correlating the serum FATP1 levels with baseline characteristics and lipid profiles in subjects with BC, age showed a positive correlation with serum FATP1 levels. Correlation of FATP1 with different receptor status in subjects with BC showed a positive correlation between FATP1 and VLDL in ER+/PR-/HER2+ receptor status, FATP1 and HDL-C showed a positive correlation in triple-negative breast cancer.</p> <p><strong>Conclusion: </strong>Though, extensive research on BC is underway, study reports on serum FATP1 levels in BC are scarce. This study demonstrates the importance of serum levels of FATP1 in BC. Further study on the molecular regulation of FATP1 in BC will add to the knowledge, and findings of the study implicates for utilizing FATP1 as a biomarker in BC.</p> RANJITHA Acharya Copyright (c) https://biomedicineonline.org/index.php/home/article/view/1916 “EFFECT OF CENTRIFUGATION FORCE AND TIME ON THE ANALYSIS OF POTASSIUM AND LACTATE DEHYDROGENASE IN SERUM – A ONE YEAR CROSS SECTIONAL STUDY” https://biomedicineonline.org/index.php/home/article/view/1915 <p><u>Introduction and aim</u>: Any imperfection which occurs at any stage during the process of testing is called laboratory error. Increasing requirements of many biochemical tests, numerous samples and automation of laboratory work are being forced to carry out at a faster speed. During centrifugation, mechanical stress caused by higher gravitational-force can induce haemolysis, which may raise the levels of analytes. Only few studies are shown to investigate the influence of settings of centrifugation of less than 10 minutes on the results of laboratory analytes. Thus, our study aimed to see the effect of centrifugation force and time on the analysis of potassium and lactate dehydrogenase. <u>Materials and methods</u>: The study was done using blood samples from 61 healthy volunteers during September 2020 – March 2021.5ml of blood sample was collected by venepuncture which was put into two separate serum tubes. Tube 1 was centrifuged for 2000g for 10 minutes, tube 2 was centrifuged for 5 minutes 3000g, and analysis was done for LDH and K+. <u>Results</u>: There was a significant difference between 5 min (3000g) and 10 min (2000g) with Lactate dehydrogenase (LDH) levels. The mean Lactate dehydrogenase (LDH) levels are significantly higher in 5 min (U/L) (3000g) as compared to 10 min (U/L) (2000g).&nbsp; The difference observed was significant, between 5 min (mmol/L) (3000g) and 10 min (mmol/L) (2000g) with Potassium (K) levels. Thus, the mean Potassium levels are significantly higher in 5 min (mmol/L) (3000g) as compared to 10 min (mmol/L) (2000g). <u>Conclusion</u>: The levels of potassium &amp; lactate dehydrogenase were found to be raised by increasing the centrifugal force to 3000g. Hence, the standard centrifugation protocol of 10 min at 2000 or 2500 rpm is to be followed in order to get the accurate results of the analytes.</p> Dr Vanishree Bubanale Copyright (c) https://biomedicineonline.org/index.php/home/article/view/1915 Prevalence and antibiogram of non-urinary isolates among patients attending a tertiary care hospital https://biomedicineonline.org/index.php/home/article/view/1914 <p><strong>Abstract</strong></p> <p>Gram-negative bacterial infections have emerged as a global problem in clinical care. The present study aimed to investigate the prevalence and antibiotic susceptibility pattern of Gram-negative bacteria (GNB) isolated from various non-urinary samples<strong>.</strong> The study was accomplished at a tertiary care hospital in Haryana. A total of 228 isolates were obtained from various clinical samples which were identified by standard bacteriological techniques. Antibiotic susceptibility testing was done using the disc diffusion technique by the&nbsp; Kirby Bauer method. Adult patients showed higher isolation of GNB (31%) as compared to pediatric patients (16.3%). The most common GNB isolated from blood&nbsp; was <em>Pseudomonas sp</em>(62%)&nbsp; and <em>Klebsiella sp</em>(66.7%) from respiratory samples.GNB isolated in our study showed resistance to common antibiotics like gentamicin and ceftriaxone. Polymyxin B and Tigecycline were found to be sensitive. Strict compliance with antimicrobial stewardship policy should be followed to reduce the menace of antibiotic resistance.</p> Sameena khan Copyright (c) https://biomedicineonline.org/index.php/home/article/view/1914