Biomedicine

Abstract

Introduction and Aim: The prospect of a successful root canal therapy relies on three main factors which includes proper instrumentation, disinfection and obturation of root canal. The goal of the present study is to analyse the anti-microbial efficacy of henna (Lawsonia inermis) against the major endodontic pathogen, Enterococcus faecalis.
Materials and Methods: In this study, the antibacterial efficacy of Lawsonia inermis is investigated against Enterococcs feacalis using the method of agar disc diffusion. Extracts of L. inermis were prepared by using methanol as an extraction solvent, whereas DMSO (Dimethyl sulfoxide) and water were used as dissolution solvents. The diluted henna sample was used as the test sample, while positive control used was chlorhexidine gluconate solution and the negative control used was saline. The bacteria were cultured, and the samples were placed to measure the zone of inhibition.
Results: Extracts of L. inermis displayed noteworthy antimicrobial activity against E. fecalis. The zone of inhibition of henna against E. fecalis was found to be 11mm whereas the positive control had 14mm.
Conclusion: In conclusion, the henna extracts had shown acceptable antimicrobial efficacy and thus this study provides the scientific reason for the use of L. inermis in dentistry.