Volume: 43 Issue: 3
Year: 2023, Page: 908-914, Doi: https://doi.org/10.51248/.v43i3.2822
Introduction and Aim: Most cases of periodontitis are associated with microorganisms. The Gram-positive bacteria Staphylococcus aureus is considered as one of the important organisms associated with periodontal infections. This study investigated the effect of silver nanoparticles as well as the antiseptic agent chlorhexidine on multi-drug resistant S. aureus isolated from periodontal infections.
Materials and Methods: In this study, with help from dentists, 266 clinical samples were collected from dental patients who had periodontal infection. S. aureus isolated from samples was tested for their antibiotic susceptibility profiles. Silver nanoparticles and chlorhexidine were evaluated for their antibacterial activity against these S. aureus isolates.
Results: S. aureus strains isolated from periodontal infection patients in this study were found to be multidrug resistant. AgNPs obtained using E. coli showed high inhibition of S. aureus growth when used in different concentrations (5, 10, 15, 20, 25mM). Chlorhexidine also exhibited antibacterial activity against S. aureus. Combination of AgNPs with penicillin and ciprofloxacin had an increasing significant effect on the sensitivity of S. aureus. Similarly, chlorhexidine in combination with penicillin and ciprofloxacin also showed an inhibitory effect on the growth of S. aureus.
Conclusion: AgNPs and chlorhexidine combined with antibiotics used in treatment of S. aureus isolated from periodontal disease showed a good antibacterial effect which suggests its use as an antibacterial agent against periodontitis associated bacteria.
Keywords: Periodontal infection; silver nanoparticles; chlorhexidine; Staphylococcus aureus; multiple drug resistance.
1. American Academy of Periodontology. Diagnosis of periodontal disease. J Periodontol 2003;74:1237-1247.
2. Smith, A.J., Jackson, M.A., Bagg, J. The ecology of Staphylococcus species in the oral cavity. J Med Microbiol 2001;50:940-946
3. Murdoch, F. E., Sammons, R. L., Chapple, I.L.C. Isolation and characterization of subgingival Staphylococci from periodontitis patients and controls. Oral Dis 2004;10:155-162.
4. Francis, A.W. Staphylococcus aureus (including toxic shock syndrome). In: Mandell GL, Bennett JE, Dolin R, editors. Principles and practices of infectious diseases. 4th ed. Philadelphia: Churchill Livingstone Elsevier. 1995;1754-1755.
5. Barkema, H. W., Schukken, Y. H., Zadoks, R.N. Invited Review: The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis. J. Dairy Sci. 2006, 89, 1877-1895.
6. Yah, C.S., Simate, G.S. Nanoparticles as potential new generation broad spectrum antimicrobial agents. Daru,2015, 23, 43.
7. Das S., Gupta V., Kalyani M.I., Kalita M.C.,
16. Chomczynski, P., Sacchi, N. The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on. Nature Protocols. 2006; 1(2): 581-585.
17. Kim, Y. Multiple antimicrobial resistance patterns of Staphylococcus aureus are isolated from periodontitis patients in Seoul, Korea. Korean J. Oral Maxillofac. Pathol. 2012; 36: 317-322.
18. Birla, S.S., Tiwari, V.V., Gade, A.K., Ingle, A.P., Yadav, A.P., Rai, M.K. Fabrication of silver nanoparticles by Phoma glomerata and its combined effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Lett Appl Microbiol. 2009;48(2):173-179.
19. Ibrahim, A.I.O., Moodley, D.S., Petrik, L., Patel, N. Use of antibacterial nanoparticles in Endodontics. Clin Rev. 2017; 72(3): 105-112.
20. Ahamed, M., Al Salhi, M.S., Siddiqui, M.K. Silver nanoparticle applications and human health. Clinica Chimica Acta. 2010; 411(23): 1841-1848.
21. Verma, R., Sharma, D. S., Pathak, A. K. Antibacterial efficacy of pastes against E. faecalis in primary root dentin: A confocal microscope study. Journal of Clinical Pediatric Dentistry. 2015; 39(3): 247-254.
22. Jamal, M.A., Rosenblatt, J.S., Hachem, R.Y., Ying, J., Pravinkumar, E., Nates, J. L., et al., Prevention of biofilm colonization by Gram-negative bacteria on minocycline-rifampin-impregnated catheters sequentially coated with chlorhexidine. J. Antimicrob Agents Chemother. 2014; 58(2): 1179-1182.
23. Lin, F., Yu, B., Wang, Q., Yuan, M., Ling, B. Combination inhibition activity of chlorhexidine and antibiotics on multidrug-resistant Acinetobacter baumannii in vitro home microorganisms. Antimicrobials Antiseptics Biological Science Microbiology Chlorhexidine. 2020 DOI:10.21203/rs.3.rs-130283/v1.
24. Jalil, I.S., Mohammad, S.Q., Mohsen, A.K. and Al-Rubaii, B.A.L. Inhibitory activity of Mentha spicata oils on biofilms of Proteus mirabilis isolated from burns. Biomedicine, 2023; 43(2):748-752.
25. Mohsin, M.R., AL-Rubaii, B.A.L., 2023. Bacterial growth and antibiotic sensitivity of Proteus mirabilis treated with anti-inflammatory and painkiller drugs. Biomedicine, 2023; 43(2):728-734.
26. Ali, M.A., Al-Rubaii, B.A. Study of the Effects of Audible Sounds and Magnetic Fields on Staphylococcus aureus Methicillin Resistance and mecA Gene Expression. Tropical Journal of Natural Product Research, 2021; 5(5): 825-830.
27. Chang, Y.N., Zhang, M., Xia, L., Zhang, J., Xing, G. The toxic effects and mechanisms of CuO and ZnO nanoparticles. Materials. 2012;5(12).
Kalita M.C., Shukla S. Sunlight driven biosynthesis of silver nanoparticles using aqueous stem extract of Tinospora sinensis (Lour.) Merr. and evaluation of its catalytic and antibacterial activity. Biomedicine. 2020 Nov 9;40(3):301-308.
9. Pratap G.K., Shantaram M. Green synthesis of silver nanoparticles (Ag-NPs) from Olea dioica Roxb., leaf extracts and its biological activity. Biomedicine. 2019;39(4):544-549.
10. Gurunathan, S., Han, J.W., Kwon, D.N., Kim, J.H. Enhanced antibacterial and anti-biofilm activities of silver nanoparticles against Gram-negative and Gram-positive bacteria. Nanoscale Res. Lett. 2014, 9, 373.
11. Al-Saidi, M.H., Al-Bana, R.J.A., Hassan, E., Al-Rubaii, B.A.L. Extraction and characterization of nickel oxide nanoparticles from Hibiscus plant using green technology and study of its antibacterial activity. Biomedicine (India). 2022; 42(6):1290–1295.
12. Saleh, T.H., Hashim, S.T., Malik, S.N., AL-Rubaii, B.A.L.Down-regulation of flil gene expression by Ag nanoparticles and TiO2 nanoparticles in pragmatic clinical isolates of Proteus mirabilis and Proteus vulgaris from urinary tract infection. Nano Biomedicine and Engineering. 2019; 11(4):321-332.
13. Clinical and Laboratory Standards Institute, Wayne. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. CLSI Document 2014; M100-S24.
14. Ratan, A., Gupta, E., Ragunathan, R. Synthesis of silver nanoparticles using Klebsiella pneumonia and its biomedical applications. International Journal of Enhanced Research in Science Technology & Engineering. 2013; 2(3): 1-7.
15. Charannya, S., Duraivel, D., Padminee, K., Poorni, S., Nishanthine, C., Srinivasan, M.R. Comparative evaluation of antimicrobial efficacy of silver nanoparticles and 2% chlorhexidine gluconate when used alone and in combination assessed using agar diffusion method: An in vitro study. Contemp Clin Dent. 2018; S2: 204-209.
Shaimaa Noori Mahal, Ahmed Mohammed Turki, Elham Hazeim Abdulkareem. Effects of silver nanoparticles on multiple drug-resistant strains of Staphylococcus aureus from periodontal infection: An alternative approach for antimicrobial therapy. Biomedicine: 2023; 43(3): 908-914