Biomedicine

Abstract

Introduction and Aim: Basil (Ocimum basilicum L.) is a popular culinary herb and its essential oils. It is used
in food products, perfumery, and dental and oral products. Basil essential oils and their principal constituents
were found to exhibit antimicrobial activity against Gram-negative and Gram-positive bacteria, yeast, and
mold. The present study aims to prepare effervescent denture cleansing granules with basil oil and to evaluate
its antibacterial activity against oral pathogens.
Materials and Methods: Citric acid, tartaric acid, and sodium bicarbonate composition were taken in a ratio
of 1: 2: 3 by weight. Different concentrations of basil oil were mixed with sodium bicarbonate initially. Both
the powders were mixed using geometric dilution, further sieved and packed in an airtight glass container.
Different concentrations of the prepared basil effervescent denture cleansing solution were tested against
Streptococcus mutans and Enterococcus faecalis The fresh bacterial suspension was dispersed on the surface
of nutrient agar plates. Different concentration of basil effervescent denture cleansing solution (50, 100 and
150 μL) was incorporated into the wells, and the plates were incubated at 37°C for 24 h. Zone of inhibition
was recorded on each plate.
Results: Basil mediated effervescent granules showed a dose-dependent antibacterial activity against the tested
microorganism with a maximum effect on Streptococcus mutans and followed by Enterococcus faecalis.
Conclusion: The prepared basil oil mediated effervescent denture cleansing granules were found to be effective
against S.mutans, E. faecalis, and Candida albicans. Hence it may be used for routine cleansing of the denture
to protect the denture users from infections such as denture stomatitis.