Volume: 43 Issue: 2
Year: 2023, Page: 615-620, Doi: https://doi.org/10.51248/.v43i02.2534
Introduction and Aim: Idiopathic infertility accounts for at least 30% of all cases of infertility, and oxidative stress has been identified as a novel developing factor in idiopathic male infertility. Oxidative stress occurs when antioxidant defense mechanisms are outmatched by the creation of reactive oxygen species (ROS). The purpose of this research was to examine the impact of enzymatic antioxidants (catalase and superoxide dismutase; CAT and SOD) on sperm DNA fragmentation and associated sperm alteration in Iraqi males with idiopathic infertility.
Materials and Methods: One hundred infertile guys (50 oligozoospermia and 50 asthenozoospermia) and 50 normospermia) had their superoxide dismutase, catalase, and malondialdehyde levels tested.
Results: Our study showed that the sperm DNA fragmentation index (DFI) of patients who suffered from oligozoospermia and asthenozoospermia was higher than that of individuals who suffered from normozoospermia. Seminal plasma SOD and MDA levels were higher in infertile participants compared to controls (P < 0.001) .The concentration of CAT in the seminal plasma of oligozoospermic and asthenozoospermic men was not significantly different from that of normospermic men. DFI correlated negatively with seminal antioxidant status (SOD, CAT) and MDA, while CAT correlated positively with SOD and MDA.
Conclusion: The impacts of oxidative stress include the breakage of DNA in sperm, with lipid peroxidation being the primary cause of idiopathic male infertility. This is associated with less successful fertility outcomes in couples. According to the findings that we obtained, the sperm DNA fragmentation index as well as malondialdehyde are substantially associated with the quality of the sperm.
Keywords: Idiopathic infertility; CAT; SOD; MDA; Sperm DNA fragmentation
1. Abd-Alwahab, M. T., Abdul Hassan, I. A., Jassim A. B. Microdeletions in Y chromosome azoospermia factor (AZF) region as affected by bacterial infection in seminal fluid of Iraqi infertile men. Biochem. Cell. Arch. 2020; 20 (2): 4381-4387.
2. Hade, I. M., Abdul Hassan, I. A. Gene expression profile of enos gene in a sample of Iraqi asthenozoo -spermic patients. Iraqi Journal of Biotechnology .2019; 1 8(3): 90-96.
3. Sharlip, I. D., Jarow, J. P., Belker, A. M., Lipshultz, L. I., Sigman, M., Thomas, A. J., et al., Best practice policies for male infertility. Fertil. Sterility. 2002;77 (5): 873-882.
4. Kadhim, Z.A. and Abdul-Hassan, I.A.The Association of mismatch repair (MLH1) gene polymorphism with the risk of oligozoospermia in Iraqi patients. Iraqi journal of biotechnology.2020; 19(1):56-68.
5. Villaverde, A.I.S.B.,Netherton, J.,Baker, M.A. From Past to Present: The link between reactive oxygen species in sperm and male infertility. Antioxidants 2019; 8: 616.
6. Ko, E. Y., Sabanegh Jr, E. S., Agarwal, A. Male infertility testing: reactive oxygen species and antioxidant capacity. Fertility and sterility, 2014; 102(6):1518-1527.
7. Smejkal, G. B., Kakumanu, S. Enzymes and their turnover numbers. Expert Review of Proteomics, 2019; 16(7): 543-544.
8. Simon, L., Murphy, K., Shamsi, M. B., Liu, L., Emery, B., Aston, K.I., et al., Paternal influence of sperm DNA integrity on early embryonic development. Hum Reprod. 2014;29(11):2402-2412.
9. Darzynkiewicz, Z. Differential staining of DNA and RNA in intact cells and isolated cell nuclei with acridine orange. Methods in Cell Biology.1990; 33:285-98.
10. Kaplan, L. A., Pesce, A. J. Operator's manual IL 943 Flame Photometer. Instrumentation Laboratory. Clinical Chemistry: Theory, analysis, and correlation,1989;2: 2-3
11. Esteves, S.C. Interventions to prevent sperm DNA damage effects on reproduction. Adv. Exp. Med. Biol. 2019; 1166:119-148.
12. Amorini, A.M., Listorti, I., Bilotta, G., Pallisco, R., Saab, M.W., Mangione, R., et al., Antioxidant-based therapies in male infertility: Do we have sufficient evidence supporting their effectiveness? Antioxidants, 2021; 10: 220-231.
13. Rehman, R., Amjad, S., Tariq, H., Zahid, N., Akhter, M., Ashraf, M. Oxidative stress and male infertility: A cross sectional study. The Journal of the Pakistan Medical Association, 2020; 70(3):461-466
14. Wagner, H., Cheng, J.W., Ko, E.Y. Role of reactive oxygen species in male infertility: An updated review of literature. Arab J Urol. 2017; 16:35-43.
15. Alahmar AT. Role of oxidative stress in male infertility: An updated review. J Hum Reprod Sci. 2019; 12(1):4-18.
16. Ayad, B.M., Oyeyipo, I.P., Van der Horst, G., Du Plessis, S.S. Cementing the relationship between conventional and advanced semen parameters. Middle East Fertility Society Journal. 2021; 26(1):1-10.
17. Riquelme, N., R., Retamal, N., H., Torres M. J., Espinosa, R.M. Catalase as a molecular target for male infertility diagnosis and monitoring: An overview. Antioxidants (Basel). 2020; 9(1): 78.
18. AL-Fleafil S. J., Al FaisalA. M., Mahood R. A. Association between GSTM1, GSTT1 Genes variants and some physiological parameters in infertility patients. Iraqi Journal of Biotechnology. 2021; 20(1):83-45.
19. Baszynski, J., Kami´nski, P., Bogdzi´nska, M., Mroczkowski, S., Szymanski, M., Wasilow, K., et al., Enzymatic antioxidant defense and polymorphic changes in male infertility. Antioxidants, 2022; 11(5):817.
20. Naif, A., Maddirevula, S., Coskun, S., Alali, H., Assoum, M., Morris, T., et al., A genomics approach to male infertility. Genetics in Medicine, 22(12):1967-1975.
21. Gill, K., Machalowski, T., Harasny, P., Kups, M., Grabowska, M., Duchnik, E., et al., Male infertility coexists with decreased sperm genomic, integrity and oxidative stress in semen
irrespective of leukocytospermia. Antioxidants, 2022; 11 (10): 1987.
22. Fadhil, H. A., AL-Ali, I. A., Abdul Wahid, H. H. Human sperm DNA fragmentation and its correlation with antioxidant markers and sperm characteristics in Iraqi males. EurAsian Journal of BioSciences . 2020; 14: 4961-4968.
23. Zribi, N., Chakroun, N.F., Elleuch, H., Abdallah, F.B., Hamida, A.S.B., Gargouri, J., et al., Sperm DNA fragmentation and oxidation are independent of malondialdheyde. Reproductive Biology and Endocrinology.2011; 9(1):1-8.
24. Aitken, R. J., Baker, M. A., Sawyer, D. Oxidative stress in the male germ line and its role in the aetiology of male infertility and genetic disease. Reproductive Biomedicine online. 2003; 7(1), 65-70.
25. Hamoode, R. H., Alkubaisy, S. A., Sattar, D. A., Hamzah, S. S., Hadi Saleh, T. H., Al-Rubaii, B.A.L. Detection of anti-testicular antibodies among infertile males using indirect immunofluorescent technique. Biomedicine. 2022; 42(5):978-982.
26. Martin-Hidalgo, D., Bragado, M.J., Batista, A.R., Oliveira, P.F., Alves, M.G. Antioxidants and male fertility: From molecular studies to clinical evidence. Antioxidants 2019; 8(4):89.
Mayasah Muthanna Khalid, Mohammed Ibrahim Nader, Rasha Abdulla Hussein Mahood. Evaluation of oxidative stress in idiopathic male infertility in the Iraqi population. Biomedicine: 2023; 43(2): 615-620