Biomedicine

Abstract

A well-known soil-borne bacterium called Ralstonia solanacearum can cause catastrophic bacterial wilt in hundreds of plant species, including vital crops like pepper, tomato, potato, banana, and eggplant. Owing to the increase in antibacterial resistance, nanotechnologists around the globe are looking for novel and eco-friendly methods of synthesis, wherein they can control these plant pathogens. Keeping in view of this statement, we aimed to green-synthesise and study the efficacy of the copper nanoparticles against the R. solanacearum. We extracted the polyphenols from fermented millets and synthesized copper nanoparticles using these extracted polyphenols. These NPs were characterized by FTIR, UV spectra and scanning electron microscopy tests. The synthesized NPs were evaluated for their antibacterial nature by well diffusion, MIC method and biofilm inhibition assay. From the broth dilution assay, we found that the green-synthesized NPs showed a greater reduction in the optical density value compared to the control. OD was found to reduce to 0.16 ±0.09 from 1.56 ±0.12 at 400μg/ml. We found the percentage of biofilm inhibition for NPs and E to be 84 and 41% respectively, at 15hr of incubation. This confirms that NPs are highly significant when compared to the positive control. These findings confirm the potential role of the NPs as antibacterial agents against plant pathogens.