Volume: 43 Issue: Supplementary 1
Year: 2023, Page: 59-65, Doi: https://doi.org/10.51248/.v43i1.2571
Introduction and aim: E-selectin, a cell adhesion molecule depicting nexus with oxidative stress in hyperglycemia has been documented in several other clinical conditions. The present study centers around the putative role of E-selectin as a biomarker of diabetic nephropathy linked to oxidative stress.
Materials and Methods: 150 type 2 diabetics in the age group of 35 - 50 years were classified into three groups, comprising 50 each, based on albuminuria. Fifty healthy age and gender matched individuals constituted the control. Extracellular E-selectin and Insulin were quantitated appropriately. Measurement of HbA1c and microalbumin were also enabled. Routine biochemistry analyses were undertaken, based on established automated procedures. The study was initiated only following approval accorded by the Institutional Ethics Committee.
Results: E-selectin levels in serum were significantly associated with Urine Albumin-to-Creatinine Ratio (UACR) and Insulin resistance in the light of estimated Glomerular Filtration Rate.
Conclusion: E-selectin levels could thus act as a putative biomarker of diabetic nephropathy linked to oxidative stress, as observed in insulin resistant type 2 diabetes
Keywords: E-selectin; type 2 diabetes mellitus (T2DM); glycemic control; insulin resistance (IR); HOMA-IR; nephropathy.
1. Garber, A. J., Handelsman, Y., Einhorn, D. Diagnosis and management of prediabetes in the continuum of hyperglycemia: when do the risks of diabetes begin? A consensus statement from The American College of Endocrinology and the American Association of Clinical Endocrinologists. EndocrPract. 2008; 14: 933-946.
2. Levey, A.S., de Jong, P.E., Coresh, J., Nahas, M., Astor, B.C. The definition, classification, and prognosis of chronic kidney disease: a KDIGO Controversies Conference report. Kidney Int. 2011; 80: 17-28.
3. Eboh, C., Tahseen, A., Chowdhury. Management of diabetic renal disease. Ann Transl Med. 2015; 3(11): 154:1-8.
4. Perkins, B.A., Ficociello,L.H., Ostrander,B.E., Silva, K.H., Weinberg, J.Microalbuminuria, and the risk for early progressive renal function decline in type 1 diabetes. J Am SocNephrol. 2007;18: 1353-1361.
5. Ceriello,A., Esposito, K., Piconi, L., Ihnat, M., Thorpe,J., Testa,R., et al., Glucose ‘peak’ and glucose ‘spike’: impact on endothelial function and oxidative stress. Diabetes Res ClinPract. 2008;82:262-267.
6. Esposito, K., Nappo, F., Marfella, R., Giugliano, G., Giugliano, F., Ciotola, M., et al., Inflammatory cytokine concentrations are acutely increased by hyperglycemia in humans: role of oxidative stress. Circulation. 2002 Oct 15; 106(16): 2067-2072.
7. Roldán,V., Marín, F., Lip, G.Y., Blann, A.D. Soluble E-selectin in cardiovascular disease and its risk factors. A review of the literature. ThrombHaemost. 2003;90(6):1007-1020.
8. Houstis,N., Rosen, E.D., Lander, E.S. Reactive oxygen species have a causal role in multiple forms of insulin resistance. Nature. 2006;440:944-948.
9. Henríquez, S., Jara, N., Bunout,D., Hirsch,S., de la Maza, M.P., Leiva, L., et al., Variability of formulas to assess insulin sensitivity and their association with the Matsuda index. Nutr Hosp. 2013;28(5):1594-1598.
10. Matthews, D.R., Hosker,J.P., Rudenski, A.S., Naylor, B.A., Treacher,D.F., Turner,R.C. Homeostasis model assessment: IR and beta-cell function from fasting plasma glucose and insulin concentration in man. Diabetologia. 1985; 28: 412-419.
11. Wallace,T.M., Levy ,J.C., Matthews ,D.R. Use and abuse of HOMA modeling. Diabetes Care. 2004;27(6):1487-1495.
12. Matthews, D. R., Hosker, J.P., Rudenski, A.S., Naylor, B.A., Treacher,D.F., Turner, R.C.Homeostasis model assessment: Insulin resistance and β-cell function from fasting plasma glucose
and insulin concentrations in man. Diabetologia 1985; 28: 412-419.
13. Hrebícek, J., Janout, V., Malincíková, J., Horáková, D., Cízek, L. Detection of insulin resistance by simple quantitative insulin sensitivity check index QUICKI for epidemiological assessment and prevention. J ClinEndocrinolMetab. 2002 Jan; 87(1):144-147.
14. Levey, A.S., Stevens, L.A., Schmid, C.H., Zhang, Y. L., Castro, A.F., Feldman, H.I.,et al., A new equation to estimate glomerular filtration rate. Annals of Internal Medicine 2009; 150 (9): 604-612.
15. Das, S.K., Roy, D.K., Chowdhury, A.A., Roy, A.S., Ahammed, S.U., Asadujjaman, M., et al., Correlation of eGFR By MDRD and CKD-EPI Formula with Creatinine Clearance Estimation in CKD Patients and Healthy Subjects. Mymensingh Med J. 2021; 30(1):35-42.
16. Laakso, M., Kuusisto, J. Insulin resistance and hyperglycaemia in cardiovascular disease development. Nat Rev Endocrinol. 2014;10(5):293-302.
17. Drew, B.G., Rye, K. A., Duffy, S. J., Barter, P., Kingwell, B.A. The emerging role of HDL in glucose metabolism.Nat Rev Endocrinol. 2012; 8(4): 237-245.
18. Wheatcroft, S.B., Williams, I.L., Shah, A.M., Kearney, M.T. Pathophysiological implications of insulin resistance on vascular endothelial function. Diabet Med. 2003; 20:255-268.
19. Surmi, B.K., Hasty, A.H. The role of chemokines in recruitment of immune cells to the artery wall and adipose tissue. Vascul. Pharmacol. 2010; 52:27-36.
20. Asaba, K., Tojo, A., Onozato, M.L., Goto, A., Quinn, M.T., Fujita, T., et al., Effects of NADPH oxidase inhibitor in diabetic nephropathy. Kidney Int. 2005; 67:1890-1898.
21. Motawi, T., Shaker, O., Taha, N., Abdel Raheem, M. Genetic variations in E-selectin and ICAM-1: relation to atherosclerosis. Med SciMonit. 2012;18(6):CR381-CR 389.
22. Bavbek, N., Kargili, A., Kaftan, O., Karakurt, F., Kosar, A., Akcay, A. Elevated concentrations of soluble adhesion molecules and large platelets in diabetic patients: are they markers of vascular disease and diabetic nephropathy? ClinApplThrombHemost. 2007; 13(4):391-397.
23. Shestakova, M.V., Kochemasova, T.V., Gorelysheva,V. A., Osipova,T.V., Polosukhina, E.P., Dedov, B.A.I.I. The role of adhesion molecules (ICAM-1 and E-selectin) in development of diabetic microangiopathies]. TerArkh. 2002;74(6):24-27.
24. Jude, E.B., Douglas, J.T., Anderson, S.G., Young, M.J., Boulton, A.J. Circulating cellular adhesion molecules ICAM-1, VCAM-1, P- and E-selectin in the prediction of cardiovascular disease in diabetes mellitus. Eur J Intern Med. 2002;13(3):185-18925. Hirata, K., Shikata, K., Matsuda, M., Akiyama, K., Sugimoto, H., Kushiro, M., et al., Increased expression of selectins in kidneys of patients with diabetic nephropathy. Diabetologia. 1998;41(2):185-192.
Meka Farid Babu, Srinivasan A. R., Benerji G. V., Ravikumar M. S. Extracellular E-selectin levels and its association with insulin resistance: Putative role of E-selectin as a biomarker of oxidative stress linked vascular manifestations in diabetic nephropathy. Biomedicine: 2023; 43(1) Supplementary issue: 59-65