Volume: 43 Issue: Supplementary 1
Year: 2023, Page: 108-113, Doi: https://doi.org/10.51248/.v43i1.2574
Introduction and Aim: Mangiferin is accompanied by the therapeutic potential for various human ailments. The present study is aimed to develop a validated HPLC method for the quantification of mangiferin in coffee (Coffea arabica) leaves.
Materials and Methods: The high-performance liquid chromatography (HPLC) was carried out on Agilent Technologies 1260 LC Infinity Series liquid chromatography system (Agilent, USA) using an isocratic mobile phase of a mixture of methanol and 1% orthophosphoric acid v/v (70:30) at a flow rate 1mL min-1 for 14 min with the controlled temperature at 25C. The mangiferin content was detected by a UV diode array detector at 258 nm.
Results: The linearity of the HPLC system was established in the range of 10-100 ppm mL-1 with a regression coefficient (R2) of 0.99670. The accuracy of the method was assessed by a study performed using 3 different levels, with a 99% average recovery. The limit of detection(LOD) and limit of quantification (LOQ) were 1.3 and 3.9 ppm mL-1. Intraday and interday precision analysis showed the relative standard deviation ≤0.012342 separately. The content of mangiferin in the coffee leaves was 94 mg/gram (w/w).
Conclusion: All necessary factors, such as linearity, accuracy, LOD, LOQ, precision, and % relative standard deviation(RSD), were inside the appropriate limits. The HPLC was validated using all necessary variables with a successful estimation of mangiferin
Keywords: HPLC; Mangiferin; coffee leaves; method validation; LOD; LOQ.
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Raghavendra L.S. Hallur, Faiyaz K. Shaikh, V.N. Magare, Prashith Kekuda T.R, Chetan D. M., Anil Kumar H.S., Manjula Shantaram. High-performance liquid chromatography method development and validation for the quantification of mangiferin in Coffea arabica leaves. Biomedicine: 2023; 43(1) Supplementary issue: 108-113