Biomedicine

Abstract

Introduction and Aim: The hypolipidemic activity, antioxidant activity and tissue fatty acid composition of high purity phytosterols (PS) obtained from rice bran oil deodorizer distillate (RBO DOD) was evaluated in free and esterified form. The phytosterol esters of ALA (PSE) were prepared by esterifying PS with alpha linolenic acid (ALA) obtained from linseed oil. Materials and Methods: Male wistar rats weighing 40-50g was used for the study. The study had six groups and each group had six animals (n=6). The serum lipid profile such as total cholesterol (TC), triacylglycerol (TAG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) was analyzed for control and treated groups. The anti-oxidant activity such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and tissue fatty acid compositions (liver, adipose, serum, brain, eye and heart) were analyzed for control and treated groups. Results: The TC, TAG and LDL-C contents in the treated groups GNO+PS, GNO+ALA, GNO+PSE-LD (lower dose), and GNO+PSE-HD (higher dose) were decreased. The HDL-C content was increased and the antioxidant enzymes (SOD and CAT) showed increased activity in the treated groups GNO+PS, GNO+ALA, GNO+PSE-LD and GNO+PSE-HD. The omega 6 to omega 3 fatty acid ratio were decreased in the treated groups such as GNO+ALA, GNO+PSE-LD and GNO+PSE-HD. Conclusions: This is probably the first study which shows hypolipidemic, antioxidant and tissue fatty acid composition of PS (isolated from RBO DOD) and ALA in individual form and also in esterified form in dose dependent study.