Volume: 43 Issue: 3
Year: 2023, Page: 897-907, Doi: https://doi.org/10.51248/.v43i3.2912
Introduction and Aim: Trichosanthes lobata is one of the species which belongs to Chinese traditional medicine for the therapeutic purpose of antioxidant properties. Free radicals’ production by the body has numerous beneficial roles including in immune systems, cellular signaling pathways, mitogenic response, and synthesis of cellular structures. This study aimed to evaluate the therapeutic efficacy of the T. lobata secondary metabolites toward COX proteins and validate their antioxidant potential.
Materials and Methods: We extracted the plant using Soxhlet and subjected it to various assays like DPPH and TEAC, followed by in silico analysis. The molecular docking and dynamic simulation have been analyzed with respect to the protein of interest against selected phytochemicals from T. lobata.
Results: We observed the significant outcome from DPPH and TEAC assays like reducing the capability in contrast to T. lobata followed by docking and dynamic stability.
Conclusion: The current findings have unveiled that the investigated flora, namely T. lobata, is a bastion of secondary phytochemicals. The plant's exceptional antioxidant capacity is attributable to the occurrence of one or more of these secondary metabolites, which exert their respective or synergistic effects
Keywords: Trichosanthes lobata; antioxidants; DPPH; molecular docking; free radicals.
1. Ray, P.D., Huang, B.W., Tsuji, Y. Reactive Oxygen Species (ROS) homeostasis and redox regulation in cellular Signaling. Cellular Signalling. 2012;24(5):981-990.
2. Das, K., Roychoudhury, A. Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Environmental Science. 2014; 2:53.
3. Sharma, P., Jha, A.B., Dubey, R.S., Pessarakli, M. Reactive Oxygen Species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany. 2012 ;2012:1-26.
4. Trachootham, D., Alexandre, J., Huang, P. Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach? Nature Reviews Drug Discovery. 2009; 8(7):579-591.
5. Rhoads, D.M. Mitochondrial Reactive Oxygen Species. Contribution to oxidative stress and interorganellar signaling. Plant Physiology. 2006;141(2): 357-366.
6. Rajasekaran, A., Periyasamy, M. Hepatoprotective effect of ethanolic extract of Trichosanthes lobata on paracetamol-induced liver toxicity in rats. Chinese Medicine. 2012; 7(1):12.
7. Bhattacharya, S., Kanti, Halder. P. Antibacterial activity of Trichosanthes diocia root. Global Journal of Pharmacology. 2010; 4(3):122-126.
8. Blois, MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181:1199-1200.
9. Lokapur, V., Jayakar, V., Divakar, M.S., Chalannavar, R.K., Lasrado, L., Shantaram, M. ZnO nanoparticles with spectroscopically controlled morphology, bioinspired from Holigarna grahamii (Wight) Kurz and delving its antioxidant and anticancer potential on A498 cell line. Materials Today Communications. 2022 Jun 1;31:103338.
10. Beauchamp, C. and Fridovich, I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry. 1971;44:276-287.
11. Manian, R., Anusuya, N., Siddhuraju, P., Manian, S. The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficus bengalensis L. and Ficus racemosa L. Food Chemistry. 2008;107(3):1000-1007.
12. Sreejayan, and Rao, M.N. Nitric oxide scavenging by curcuminoids. Journal of Pharmacy and Pharmacology. 1997;49:105-107.
13. Jose, M., Varghese, V.I., Jayakar, V., Lokapur, V., Srinivasa, K., Shantaram, M. Evaluation of antioxidant activities and free radical scavenging properties in mango leaves, husks of areca and coconut. Research Journal of Pharmaceutical Biological and Chemical Sciences. 2018 Jul 1;9(4):1607-1619.
14. Klein, S.M., Cohen, G., Cederbaum, A.I. Production of formaldehyde during metabolism of dimethyl sulfoxide by hydroxyl radical-generating systems. Biochemistry. 1981; 20(21): 6006-6012.
15. Ohkawa, H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2):351-8
16. Prieto, P., Pineda, M. and Aguilar, M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry. 1999; 269: 337-341.
17. Lokapur, V., Jayakar, V., Shantaram, M. Preliminary phytochemical screening, physicochemical analysis and in-vitro antioxidant activity of selected Holigarna species-
Endemic plant species of Western Ghats. Biomedicine. 2020;40(4):460-466.
18. Dinis T.C.P., Madeira V.M.C., Almeida L.M. Action of phenolic derivatives (acetaminophen, salicylate and 5-amino salicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics. 1994;315:161-169.
19. Wong, F.C., Yong, A.L., Ting, E.P., Khoo, S.C., Ong, H.C., Chai, T.T. Antioxidant, metal chelating, anti-glucosidase activities and phytochemical analysis of selected tropical medicinal plants. Iranian Journal of Pharmaceutical Research. 2014. 13(4):1409-1415.
20. Boonchum, W., Peerapornpisal, Y., Kanjanapothi, D., Pekkoh, J., Pumas, C., Jamjia, U., et al., Antioxidant activity of some seaweed from the Gulf of Thailand. International Journal of Agriculture and Biology. 2011;13(1):95-99.
21. Ebrahimzadeh, M.A., Pourmorad, F., Hafezi, S. Antioxidant activities of Iranian corn silk. Turkish Journal of Biology. 2008;32:43-49.
22. Khan, R.A., Khan, M.R., Sahreen, S., Ahmed, M. Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens. Chemistry Central Journal. 2012 May 22;6(1).
23. Desai, S.J., Prickril, B., Rasooly, A. Mechanisms of phytonutrient modulation of cyclooxygenase-2 (COX-2) and inflammation related to cancer. Nutrition and Cancer. 2018 ; 70(3):350-375.
24. Ashok, A., Andrabi, S.S., Mansoor, S., Kuang, Y., Kwon, B.K., Labhasetwar, V. Antioxidant therapy in oxidative stress-induced neurodegenerative diseases: Role of nanoparticle-based drug delivery systems in clinical translation. Antioxidants. 2022; 11(2):408.
Aghil Soorya Aravindakshan, Raghavendra L.S. Hallur, Sameer Sharma. In silico analysis of Trichosanthes lobata extracts: A promising source of antioxidants for therapeutic applications. Biomedicine: 2023; 43(3): 897-907