Volume: 43 Issue: Supplementary 1
Year: 2023, Page: 140-145, Doi: https://doi.org/10.51248/.v43i1.1676
Introduction and Aim: Andrographolide is one of the main constituents of Andrographis paniculata. An anti-collagenase property of andrographolide is yet to be explored. Thus, the primary goal of study is to assess the anti-collagenase property of andrographolides against standard bacterial collagenase. The secondary objective is to evaluate the molecular docking between andrographolide and proteins of periodontal pathogens [P. gingivalis (Arg and Lys Gingipains), P. intermedia and A. actinomycetecomitans].
Materials and Methods: Anti-collagenase property of an andrographolide was determined using a UV spectrophotometer at four different concentrations (0.5%, 1%, 2.5% and 5%) against bacterial collagenase. Furthermore, an in-silico molecular docking study was performed between andrographolide and the proteins of periodontal pathogens using the Molegro Virtual Docker (MVD) software.
Results: Different concentrations of andrographolide had a collagenase inhibiting property of upto 65% at an IC50 value of 2.4μg/ml at the end of 20mins. Based on molecular docking, the proteins exhibited high binding energy to the bacterial enzymes.
Conclusion: Within the limitations of this study andrographolides can inhibit bacterial collagenase in an in-vitro model. Also, Andrographolide was successfully docked in the protein binding sites of P. gingivalis (Arg and Lys Gingipains), P. intermedia and A. actinomycetecomitans indicating its ability to interact with these proteins.
Keywords: Andrographolide; collagenase; immunomodulation; periodontitis; molecular docking
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Madhumathi D., Ram Sabarish, Srikanth J., S.K. Balaji. In-vitro and in-silico approach to assess the bacterial collagenase inhibiting
property of andrographolide. Biomedicine: 2023; 43(1) Supplementary issue: 140-145