Biomedicine

Abstract

Aim:We used the Indian white perch tiger, one of the common fish found in the fish waste, to extract acid soluble collagen (ASC), to increase the utilization of fish waste from the fish industries; and to focus on the natural raw materials in cosmetics, as they are less harmful to the skin, and investigated some biochemical properties. Result: The yield of isolated ASC was found to be 0.521%. The sodium dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) study showed that the isolated collagen was Type-I and consisted of two α subunits1 and 2 respectively, with a molecular weight of approximately 148 kDa. The ultraviolet (UV) absorption spectrum of collagen showed absorption at 226 nm. The Fourier transform infrared spectroscopy (FT-IR) spectrum of ASC showed the peaks for Amide-I, II, and III corresponding to functional groups of the protein. The high absorption peak was observed at 226 nm, corresponding to C=O, COOH, and CONH2 from the Ultraviolet spectrometer. Through all these confirmatory studies, it was concluded that the extracted collagen was found to be Type-1 and it was found to be linear with the similar studies carried out for different marine sources. Collagen's ability to absorb and hold moisture was examined in this study, and it was discovered to be superior to the control substance, glycerol. Because of this, the collagen from Indian white perch tigers may be used as a moisturizer and may have applications in the cosmetic industry.