Biomedicine

Abstract

Introduction and Aim: The detection of pathogenic strains of Pseudomonas aeruginosa is usually carried out by antimicrobial susceptibility testing, molecular characterisation, selective isolation, 16S rRNA sequencing, immunological assays and through other methods. This study aimed to use mushroom as a host model for detecting pathogenic strains of P. aeruginosa in wound infection.
Materials and Methods: The strains of P. aeruginosa from wound infection were isolated. The pathogenic, non-pathogenic and ATCC strains of P. aeruginosa as well as Escherichia coli were injected respectively into the cap region of the mushroom and incubated for the production of lesion. The collagen was extracted from mushroom by salt precipitation method and pure collagen was purchased commercially. The collagen degradation by all the above organisms was detected by performing UV spectrophotometric analysis. The organisms were allowed to grow on medium containing extracted collagen and pure collagen.
Results: The lesions were found at the site of the injected region on mushroom cap when injected with pathogenic strain of P. aeruginosa while the rest of the strains failed to produce lesion. The collagen was degraded when infected with the pathogenic strain and the rest of the strains failed to degrade collagen. This is in accordance with the OD value. The medium containing collagen also showed growth only for pathogenic strain of P. aeruginosa.
Conclusion: Thus, the study proved that mushroom can be used as a host model to differentiate the pathogenic and non-pathogenic strains of P. aeruginosa from wound infections.