Due to transition in the journal platform, the previously submitted articles, which are under process can be re-submitted here for quick process, kindly co-operate

Biomedicine

Volume: 39 Issue: 4

  • Open Access
  • Original Article

Synthesis, anticancer and antimitotic activity of analogues of podophyllotoxin on B16F10 melanoma cell lines and Allium cepa L.

Sathisha A. D.1, 2, Hemakumar K. H.2, Sophiya P.4, Giresha A. S.4, Lohith N. S.4, Basavaraju Y. B2. Dharmappa K. K.3, 4

1Department of Medical Biochemistry and Molecular Biology, School of Medicine, College of Health Sciences, Mekelle University, Mekelle, Ethiopia
2Department of Chemistry, 3Department of Studies and Research in Biochemistry, Manasagangotri, University of Mysore, Mysore, India 4Department of Studies and Research in Biochemistry, Mangalore University Jnana Kaveri Post Graduate Centre, Chikka Aluvara, Kodagu, Karnataka, India

Corresponding author: Dharmappa K. K. email: [email protected], [email protected]

Year: 2019, Page: 571-579, Doi: https://doi.org/10.51248/v39i4.12

Abstract

Introduction and Aim: As a timely need for potent anticancer drugs, we attempted to synthesize analogues of podophyllotoxin which are related to etoposide and tenoposide presently in the market.
Materials and Methods: Compounds 8-13 were synthesized at standard condition by modifying the ring C structure of the parent podophyllotoxin and characterized by IR, NMR, Mass spectra and elemental analysis. Anticancer (MTT assay) activity for synthetic compounds was carried out on B16F10 mouse melanoma cell lines and antimitotic activity (cytotoxic) assay was on mitotic cells of root tips of Allium cepa L.
Results: Analogues 8 and 9 exhibited greater anticancer activity with the IC50 values of 1.6 and 1.75mM respectively, and strong inhibition of mitosis with the ID50 values of 1.85mM and 2.10mM respectively, whereas the analogues 10, 11, 12 and 13 showed moderate anticancer activity.
Conclusion: Analogues 8 and 9 would become the novel anticancer drugs in future for cancer chemotherapy after further investigations.

Keywords: Synthesis; podophyllotoxin; anticancer; antimitotic.

Cite this article

Sathisha A. D., Hemakumar K. H., Sophiya P., Giresha A. S., Lohith N. S., Basavaraju Y. B. Dharmappa K. K. Synthesis, anticancer and antimitotic activity of analogues of podophyllotoxin on B16F10 melanoma cell lines and Allium cepa L. Biomedicine: 2019; 39(4): 571- 579

Views
119
Downloads
267
Citations