Biomedicine

Abstract

Introduction and aim: Actinomycetes are widely recognized for their remarkable ability to produce a wide array of secondary metabolites, including antibiotics, antitumor agents, and other biologically active compounds. The document emphasizes the ubiquity of these filamentous microorganisms in soil and highlights the screening of actinomycetes for antimicrobial activity, involving primary and secondary screening methods. It details the steps involved in primary screening, such as the cross-streak assay method, and the subsequent secondary screening for antibiotic production. The selection of actinomycete strains for mass cultivation and the extraction of antimicrobial metabolites is also outlined. The aim of the study is to shed light on the multifaceted role of actinomycetes, encompassing their antimicrobial properties, through a comprehensive exploration of the isolation, purification, and characterization of actinomycetes, the document aims to provide valuable insights into their potential as sources of bioactive compounds and their significance in addressing challenges such as antimicrobial resistance and the need for new therapeutic agents. Materials and Methods: The study addresses the antagonistic activity of actinomycetes against various pathogenic bacteria, emphasizing their potential in combating drug-resistant pathogens and also provides a detailed account of the methods used to assess antagonistic activity, including agar diffusion methods. Results: The results highlights the significant findings of the study, including the isolation, purification, and characterization of actinomycetes from soil samples, emphasizing their antimicrobial activity against pathogenic strains. Conclusion: The study concludes that actinomycetes exhibit significant biodiversity and possess strong antibacterial properties, particularly against drug-resistant bacteria. The isolation and characterization of these microorganisms highlight their potential as sources of new antibacterial compounds and their role in addressing antibacterial resistance.