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Biomedicine

Volume: 44 Issue: 1

  • Open Access
  • Original Article

The effect of ginger extract on expression of Gli1 and Patched-1 genes involved in breast cancer signaling pathway under in vitro condition

Seyedeh Mahsa Torabi1, Mahdi Rahaie2, Asa Ebrahimi1

1Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran

Corresponding author: Mahdi Rahaie. Email: [email protected]

Year: 2024, Page: 107-113, Doi: https://doi.org/10.51248/.v44i1.4152

Received: Dec. 5, 2023 Accepted: Feb. 1, 2024 Published: May 1, 2024

Abstract

Introduction and Aim: Breast cancer ranks among the leading causes of death in women worldwide. Ginger has shown potential efficacy against certain cancer types, surpassing conventional therapies such as chemotherapy and radiation. However, its molecular mechanisms remain less understood.
Materials and Methods: In this study, MCF-7 cancer cells were cultured and treated with various concentrations of aqueous ginger extract (20, 30, 45, 65 μg/mL) for 12, 48, and 72 hours. The effects were assessed through gene expression analysis and cell vitality assays (MTT).
Results: The cell vitality test revealed a direct correlation between cytotoxicity and extract concentration. Concentrations exceeding 30 μg/mL exhibited significant cell death (IC50 of 104.03 μg/mL). Gene expression analysis demonstrated an increase in Patched-1 and a decrease in Gli1 expression with rising extract concentrations. The maximum Patched-1 expression occurred at 65 μg/mL after 72 hours. Patched-1 (oncogene) and Gli1 (tumor suppressor) are pivotal genes in the hedgehog (Hh) signalling pathway associated with breast cancer.
Conclusion: It appears that ginger compounds play a substantial role in regulating cancer progression by influencing key components of the hedgehog signalling pathway.

Keywords: Ginger extract; breast cancer; gene expression analysis; Patched-1; Gli1.

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Cite this article

Seyedeh Mahsa Torabi, Mahdi Rahaie, Asa Ebrahimi. The effect of ginger extract on expression of Gli1 and Patched-1 genes involved in breast cancer signaling pathway under in vitro condition. Biomedicine: 2024; 44(1): 107-113

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